Profiling functions of ectomycorrhizal diversity and root structuring in seedlings of Norway spruce (Picea abies) with fast- and slow-growing phenotypes

Sannakajsa M. Velmala, Tiina Rajala, Jussi Heinonsalo, Andrew F. S. Taylor, Taina Penannen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We studied the role of taxonomical and functional ectomycorrhizal (ECM) fungal diversityin root formation and nutrient uptake by Norway spruce (Picea abies) seedlings with fast- andslow-growing phenotypes.

Seedlings were grown with an increasing ECM fungal diversity gradient from one to fourspecies and sampled before aboveground growth differences between the two phenotypeswere apparent. ECM fungal colonization patterns were determined and functional diversitywas assayed via measurements of potential enzyme activities of eight exoenzymes probablyinvolved in nutrient mobilization.

Phenotypes did not vary in their receptiveness to different ECM fungal species. However,seedlings of slow-growing phenotypes had higher fine-root density and thus more condensedroot systems than fast-growing seedlings, but the potential enzyme activities of ectomycor-rhizas did not differ qualitatively or quantitatively. ECM species richness increased host nutri-ent acquisition potential by diversifying the exoenzyme palette. Needle nitrogen contentcorrelated positively with high chitinase activity of ectomycorrhizas.

Rather than fast- and slow-growing phenotypes exhibiting differing receptiveness to ECMfungi, our results suggest that distinctions in fine-root structuring and in the belowgroundgrowth strategy already apparent at early stages of seedling development may explain latergrowth differences between fast- and slow-growing families.
Original languageEnglish
Pages (from-to)610-622
Number of pages13
JournalNew Phytologist
Volume201
Issue number2
Early online date14 Oct 2013
DOIs
Publication statusPublished - Jan 2014

Fingerprint

Abies
Picea
Norway
Seedlings
Picea abies
Phenotype
phenotype
seedlings
enzyme activity
Chitinases
ectomycorrhizae
Enzymes
chitinase
nutrient uptake
Needles
Nitrogen
species diversity
Food
nutrients
nitrogen

Keywords

  • ectomycorrhizal diversity
  • extracellular enzyme activities
  • fast- and slow-growing seedlings
  • fine roots
  • Norway spruce (Picea abies (L.) Karst)
  • Piloderma sp.
  • Tylospora asterophora
  • Wilcoxina sp

Cite this

Profiling functions of ectomycorrhizal diversity and root structuring in seedlings of Norway spruce (Picea abies) with fast- and slow-growing phenotypes. / Velmala, Sannakajsa M.; Rajala, Tiina; Heinonsalo, Jussi; Taylor, Andrew F. S.; Penannen, Taina.

In: New Phytologist, Vol. 201, No. 2, 01.2014, p. 610-622.

Research output: Contribution to journalArticle

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abstract = "We studied the role of taxonomical and functional ectomycorrhizal (ECM) fungal diversityin root formation and nutrient uptake by Norway spruce (Picea abies) seedlings with fast- andslow-growing phenotypes.Seedlings were grown with an increasing ECM fungal diversity gradient from one to fourspecies and sampled before aboveground growth differences between the two phenotypeswere apparent. ECM fungal colonization patterns were determined and functional diversitywas assayed via measurements of potential enzyme activities of eight exoenzymes probablyinvolved in nutrient mobilization.Phenotypes did not vary in their receptiveness to different ECM fungal species. However,seedlings of slow-growing phenotypes had higher fine-root density and thus more condensedroot systems than fast-growing seedlings, but the potential enzyme activities of ectomycor-rhizas did not differ qualitatively or quantitatively. ECM species richness increased host nutri-ent acquisition potential by diversifying the exoenzyme palette. Needle nitrogen contentcorrelated positively with high chitinase activity of ectomycorrhizas.Rather than fast- and slow-growing phenotypes exhibiting differing receptiveness to ECMfungi, our results suggest that distinctions in fine-root structuring and in the belowgroundgrowth strategy already apparent at early stages of seedling development may explain latergrowth differences between fast- and slow-growing families.",
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note = "This work was funded by the Academy of Finland project number 128229. We are grateful for the work of Marja-Leena Napola, Satu Peltola, the staff of Haapastensyrj{\"a} nursery, Arja Tervahauta, Minna Oksanen, Matias H{\"a}yrynen, Vesa Hautala, Ritva Vanhanen, Adriana Villaverde Monar, Laura H{\"a}nninen and Tatu Uutela. We thank Dr Audrius Menkis for providing us with the Wilcoxina sp. isolate. We are thankful to three anonymous referees for their suggestions which greatly improved the manuscript.",
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AU - Heinonsalo, Jussi

AU - Taylor, Andrew F. S.

AU - Penannen, Taina

N1 - This work was funded by the Academy of Finland project number 128229. We are grateful for the work of Marja-Leena Napola, Satu Peltola, the staff of Haapastensyrjä nursery, Arja Tervahauta, Minna Oksanen, Matias Häyrynen, Vesa Hautala, Ritva Vanhanen, Adriana Villaverde Monar, Laura Hänninen and Tatu Uutela. We thank Dr Audrius Menkis for providing us with the Wilcoxina sp. isolate. We are thankful to three anonymous referees for their suggestions which greatly improved the manuscript.

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N2 - We studied the role of taxonomical and functional ectomycorrhizal (ECM) fungal diversityin root formation and nutrient uptake by Norway spruce (Picea abies) seedlings with fast- andslow-growing phenotypes.Seedlings were grown with an increasing ECM fungal diversity gradient from one to fourspecies and sampled before aboveground growth differences between the two phenotypeswere apparent. ECM fungal colonization patterns were determined and functional diversitywas assayed via measurements of potential enzyme activities of eight exoenzymes probablyinvolved in nutrient mobilization.Phenotypes did not vary in their receptiveness to different ECM fungal species. However,seedlings of slow-growing phenotypes had higher fine-root density and thus more condensedroot systems than fast-growing seedlings, but the potential enzyme activities of ectomycor-rhizas did not differ qualitatively or quantitatively. ECM species richness increased host nutri-ent acquisition potential by diversifying the exoenzyme palette. Needle nitrogen contentcorrelated positively with high chitinase activity of ectomycorrhizas.Rather than fast- and slow-growing phenotypes exhibiting differing receptiveness to ECMfungi, our results suggest that distinctions in fine-root structuring and in the belowgroundgrowth strategy already apparent at early stages of seedling development may explain latergrowth differences between fast- and slow-growing families.

AB - We studied the role of taxonomical and functional ectomycorrhizal (ECM) fungal diversityin root formation and nutrient uptake by Norway spruce (Picea abies) seedlings with fast- andslow-growing phenotypes.Seedlings were grown with an increasing ECM fungal diversity gradient from one to fourspecies and sampled before aboveground growth differences between the two phenotypeswere apparent. ECM fungal colonization patterns were determined and functional diversitywas assayed via measurements of potential enzyme activities of eight exoenzymes probablyinvolved in nutrient mobilization.Phenotypes did not vary in their receptiveness to different ECM fungal species. However,seedlings of slow-growing phenotypes had higher fine-root density and thus more condensedroot systems than fast-growing seedlings, but the potential enzyme activities of ectomycor-rhizas did not differ qualitatively or quantitatively. ECM species richness increased host nutri-ent acquisition potential by diversifying the exoenzyme palette. Needle nitrogen contentcorrelated positively with high chitinase activity of ectomycorrhizas.Rather than fast- and slow-growing phenotypes exhibiting differing receptiveness to ECMfungi, our results suggest that distinctions in fine-root structuring and in the belowgroundgrowth strategy already apparent at early stages of seedling development may explain latergrowth differences between fast- and slow-growing families.

KW - ectomycorrhizal diversity

KW - extracellular enzyme activities

KW - fast- and slow-growing seedlings

KW - fine roots

KW - Norway spruce (Picea abies (L.) Karst)

KW - Piloderma sp.

KW - Tylospora asterophora

KW - Wilcoxina sp

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SN - 0028-646X

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