Profiling of mitochondrial associated proteins from rat colon

Sara Padidar, Charles Bestwick, Tim P. King, Garry Jonathan Rucklidge, Gary James Duncan, Martin David Reid, Janice Drew

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mitochondrial dysfunction, damage and mutations of mitochondrial proteins give rise to a range of ill understood patterns of disease. Although there is significant general knowledge of the proteins and the functional processes of the mitochondria, there is little knowledge of difference about how mitochondria respond and how they are regulated in different organs and tissues. Proteomic profiling of mitochondria and associated proteins involved in mitochondrial regulation and trafficking within cells and tissues has the potential to provide insights into mitochondrial dysfunction associated with many human diseases. The rat colon mitoproteome analysis presented here provides a useful tool to assist in identification and interpretation of mitochondrial dysfunction implicated in colon pathogenesis. 2DPAGE followed by LC/MS/MS was used to identify 430 proteins from mitochondrial enriched fractions prepared from rat colon, resulting in 195 different proteins or approximately 50% of the resolved proteins being identified as multiple protein expression forms. Proteins associated with the colon mitoproteome were involved in calcium binding, cell cycle, energy metabolism and electron transport chain, protein folding, protein synthesis and degradation, redox regulation, structural proteins, signalling and transporter and channel proteins. The mitochondrial associated proteins identified in this study of colon tissue complement and are compared with other recently published mitoproteome analyses from other organ tissues, and will assist in revealing potentially organ specific roles of the mitochondria and organ specific disease associated with mitochondrial dysfunction.

Original languageEnglish
Pages (from-to)78-97
Number of pages20
JournalJournal of Cellular Biochemistry
Volume103
Issue number1
Early online date11 May 2007
DOIs
Publication statusPublished - 1 Jan 2008

Keywords

  • organelle proteomics
  • electron transport chain
  • mitochondrial dysfunction
  • flow cytometry
  • transmission electron microscopy
  • modulates oxidative stress
  • mucosal pentraxin MPTX
  • carcinoma-cells
  • liquid-chromatography
  • experimental colitis
  • proteomic analysis
  • apoptosis
  • cancer
  • membrane
  • heart

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