Profiling of secondary metabolite gene clusters regulated by LaeA in Aspergillus niger FGSC A1279 based on genome sequencing and transcriptome analysis

Bin Wang; Yangyong Lv; Xuejie Li; Yiying Lin; Hai Deng; Li Pan

doi:10.1016/j.resmic.2017.10.002

Profiling of secondary metabolite gene clusters regulated by LaeA in Aspergillus niger FGSC A1279 based on genome sequencing and transcriptome analysis

Bin Wang, Yangyong Lv, Xuejie Li, Yiying Lin, Hai Deng, Li Pan

Chemistry

South China University of Technology

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

The global regulator LaeA controls the production of many fungal secondary metabolites, possibly via chromatin remodeling. Here we aimed to survey the secondary metabolite profile regulated by LaeA in Aspergillus niger FGSC A1279 by genome sequencing and comparative transcriptomics between the laeA deletion (ΔlaeA) and overexpressing (OE-laeA) mutants. Genome sequencing revealed four putative polyketide synthase genes specific to FGSC A1279, suggesting that the corresponding polyketide compounds might be unique to FGSC A1279. RNA-seq data revealed 281 putative secondary metabolite genes upregulated in the OE-laeA mutants, including 22 secondary metabolite backbone genes. LC-MS chemical profiling illustrated that many secondary metabolites were produced in OE-laeA mutants compared to wild type and ΔlaeA mutants, providing potential resources for drug discovery. KEGG analysis annotated 16 secondary metabolite clusters putatively linked to metabolic pathways. Furthermore, 34 of 61 Zn2Cys6 transcription factors located in secondary metabolite clusters were differentially expressed between ΔlaeA and OE-laeA mutants. Three secondary metabolite clusters (cluster 18, 30 and 33) containing Zn2Cys6 transcription factors that were upregulated in OE-laeA mutants were putatively linked to KEGG pathways, suggesting that Zn2Cys6 transcription factors might play an important role in synthesizing secondary metabolites regulated by LaeA. Taken together, LaeA dramatically influences the secondary metabolite profile in FGSC A1279.

Original language	English
Pages (from-to)	67-77
Number of pages	11
Journal	Research in Microbiology
Volume	169
Issue number	2
Early online date	18 Oct 2017
DOIs	https://doi.org/10.1016/j.resmic.2017.10.002
Publication status	Published - 28 Feb 2018

Bibliographical note

Acknowledgements: This work was supported by the Science and Technology Planning Project of Guangdong Province (grant numbers 2016A050503016 and 2016A010105004); the Natural Science Foundation of Guangdong Province (grant number 2017A030313097); the Science and Technology Planning Project of Guangzhou City (grant number 201510010191); Fundamental Research Funds for the Central Universities (grant number 2015ZP032); the Natural Science Foundation of Guangdong Province (grant number S2012030006235); and the Key Laboratory of Molecular Microbiology and Technology (Ministry of Education, Nankai University, China).

Keywords

Journal Article
Aspergillus niger
secondary metabolites
LaeA
comparative genomics
transcriptomics
Zn2Cys6 transcription factor

Access to Document

10.1016/j.resmic.2017.10.002Licence: Unspecified

Cite this

@article{9782cd2bcb904868b5ee71b76b2a4ca4,

title = "Profiling of secondary metabolite gene clusters regulated by LaeA in Aspergillus niger FGSC A1279 based on genome sequencing and transcriptome analysis",

abstract = "The global regulator LaeA controls the production of many fungal secondary metabolites, possibly via chromatin remodeling. Here we aimed to survey the secondary metabolite profile regulated by LaeA in Aspergillus niger FGSC A1279 by genome sequencing and comparative transcriptomics between the laeA deletion (ΔlaeA) and overexpressing (OE-laeA) mutants. Genome sequencing revealed four putative polyketide synthase genes specific to FGSC A1279, suggesting that the corresponding polyketide compounds might be unique to FGSC A1279. RNA-seq data revealed 281 putative secondary metabolite genes upregulated in the OE-laeA mutants, including 22 secondary metabolite backbone genes. LC-MS chemical profiling illustrated that many secondary metabolites were produced in OE-laeA mutants compared to wild type and ΔlaeA mutants, providing potential resources for drug discovery. KEGG analysis annotated 16 secondary metabolite clusters putatively linked to metabolic pathways. Furthermore, 34 of 61 Zn2Cys6 transcription factors located in secondary metabolite clusters were differentially expressed between ΔlaeA and OE-laeA mutants. Three secondary metabolite clusters (cluster 18, 30 and 33) containing Zn2Cys6 transcription factors that were upregulated in OE-laeA mutants were putatively linked to KEGG pathways, suggesting that Zn2Cys6 transcription factors might play an important role in synthesizing secondary metabolites regulated by LaeA. Taken together, LaeA dramatically influences the secondary metabolite profile in FGSC A1279.",

keywords = "Journal Article, Aspergillus niger, secondary metabolites, LaeA, comparative genomics, transcriptomics, Zn2Cys6 transcription factor",

author = "Bin Wang and Yangyong Lv and Xuejie Li and Yiying Lin and Hai Deng and Li Pan",

note = "Acknowledgements: This work was supported by the Science and Technology Planning Project of Guangdong Province (grant numbers 2016A050503016 and 2016A010105004); the Natural Science Foundation of Guangdong Province (grant number 2017A030313097); the Science and Technology Planning Project of Guangzhou City (grant number 201510010191); Fundamental Research Funds for the Central Universities (grant number 2015ZP032); the Natural Science Foundation of Guangdong Province (grant number S2012030006235); and the Key Laboratory of Molecular Microbiology and Technology (Ministry of Education, Nankai University, China).",

year = "2018",

month = feb,

day = "28",

doi = "10.1016/j.resmic.2017.10.002",

language = "English",

volume = "169",

pages = "67--77",

journal = "Research in Microbiology",

issn = "0923-2508",

publisher = "Elsevier Masson SAS",

number = "2",

}

TY - JOUR

T1 - Profiling of secondary metabolite gene clusters regulated by LaeA in Aspergillus niger FGSC A1279 based on genome sequencing and transcriptome analysis

AU - Wang, Bin

AU - Lv, Yangyong

AU - Li, Xuejie

AU - Lin, Yiying

AU - Deng, Hai

AU - Pan, Li

N1 - Acknowledgements: This work was supported by the Science and Technology Planning Project of Guangdong Province (grant numbers 2016A050503016 and 2016A010105004); the Natural Science Foundation of Guangdong Province (grant number 2017A030313097); the Science and Technology Planning Project of Guangzhou City (grant number 201510010191); Fundamental Research Funds for the Central Universities (grant number 2015ZP032); the Natural Science Foundation of Guangdong Province (grant number S2012030006235); and the Key Laboratory of Molecular Microbiology and Technology (Ministry of Education, Nankai University, China).

PY - 2018/2/28

Y1 - 2018/2/28

N2 - The global regulator LaeA controls the production of many fungal secondary metabolites, possibly via chromatin remodeling. Here we aimed to survey the secondary metabolite profile regulated by LaeA in Aspergillus niger FGSC A1279 by genome sequencing and comparative transcriptomics between the laeA deletion (ΔlaeA) and overexpressing (OE-laeA) mutants. Genome sequencing revealed four putative polyketide synthase genes specific to FGSC A1279, suggesting that the corresponding polyketide compounds might be unique to FGSC A1279. RNA-seq data revealed 281 putative secondary metabolite genes upregulated in the OE-laeA mutants, including 22 secondary metabolite backbone genes. LC-MS chemical profiling illustrated that many secondary metabolites were produced in OE-laeA mutants compared to wild type and ΔlaeA mutants, providing potential resources for drug discovery. KEGG analysis annotated 16 secondary metabolite clusters putatively linked to metabolic pathways. Furthermore, 34 of 61 Zn2Cys6 transcription factors located in secondary metabolite clusters were differentially expressed between ΔlaeA and OE-laeA mutants. Three secondary metabolite clusters (cluster 18, 30 and 33) containing Zn2Cys6 transcription factors that were upregulated in OE-laeA mutants were putatively linked to KEGG pathways, suggesting that Zn2Cys6 transcription factors might play an important role in synthesizing secondary metabolites regulated by LaeA. Taken together, LaeA dramatically influences the secondary metabolite profile in FGSC A1279.

AB - The global regulator LaeA controls the production of many fungal secondary metabolites, possibly via chromatin remodeling. Here we aimed to survey the secondary metabolite profile regulated by LaeA in Aspergillus niger FGSC A1279 by genome sequencing and comparative transcriptomics between the laeA deletion (ΔlaeA) and overexpressing (OE-laeA) mutants. Genome sequencing revealed four putative polyketide synthase genes specific to FGSC A1279, suggesting that the corresponding polyketide compounds might be unique to FGSC A1279. RNA-seq data revealed 281 putative secondary metabolite genes upregulated in the OE-laeA mutants, including 22 secondary metabolite backbone genes. LC-MS chemical profiling illustrated that many secondary metabolites were produced in OE-laeA mutants compared to wild type and ΔlaeA mutants, providing potential resources for drug discovery. KEGG analysis annotated 16 secondary metabolite clusters putatively linked to metabolic pathways. Furthermore, 34 of 61 Zn2Cys6 transcription factors located in secondary metabolite clusters were differentially expressed between ΔlaeA and OE-laeA mutants. Three secondary metabolite clusters (cluster 18, 30 and 33) containing Zn2Cys6 transcription factors that were upregulated in OE-laeA mutants were putatively linked to KEGG pathways, suggesting that Zn2Cys6 transcription factors might play an important role in synthesizing secondary metabolites regulated by LaeA. Taken together, LaeA dramatically influences the secondary metabolite profile in FGSC A1279.

KW - Journal Article

KW - Aspergillus niger

KW - secondary metabolites

KW - LaeA

KW - comparative genomics

KW - transcriptomics

KW - Zn2Cys6 transcription factor

U2 - 10.1016/j.resmic.2017.10.002

DO - 10.1016/j.resmic.2017.10.002

M3 - Article

C2 - 29054463

SN - 0923-2508

VL - 169

SP - 67

EP - 77

JO - Research in Microbiology

JF - Research in Microbiology

IS - 2

ER -

Profiling of secondary metabolite gene clusters regulated by LaeA in Aspergillus niger FGSC A1279 based on genome sequencing and transcriptome analysis

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this