Proliferative responses of rainbow trout, Oncorhynchusmykiss, T and B cells to antigens of Aeromonas salmonicida

M. J. Marsden, S. H. Hamdani, C. J. Secombes*

*Corresponding author for this work

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A mouse monoclonal anti-trout Ig (I-14) was used in a panning technique toseparate rainbow trout, Oncorhynchus mykiss, peripheral blood leucocytes (PBL) into sIg- (T) and sIg+ (B) cell populations. The ability of these separated populations and unfractionated PBL to proliferate to various whole cell antigen preparations of A. salmonicida was investigated, using cells from fish injected previously with an A-layer negative strain (MT004) grown under iron-restricted conditions or saline (controls). All primed cell populations demonstrated enhanced responses to A. salmonicida antigens above their respective controls, indicative of both T and B memory cell formation in vaccinated individuals. Interestingly, both T and B cell populations from primed fish were also able to respond to an A-layer positive strain (MT423) of A. salmonicida, suggesting that both forms of bacteria share common immunogenic T and B cell epitopes. However, responses to the A-layer positive strain by cells from both primed and control fish were found to be of a lower magnitude than those to the same dose of the A-layer negative strain, particularly at the lower doses used to stimulate proliferation in vitro. Proliferative responses of these PBL populations to whole A. salmonicida preparations, grown with or without iron restriction, were also generally slightly higher using iron-restricted bacteria but not significantly so.

Original languageEnglish
Pages (from-to)199-210
Number of pages12
JournalFish and Shellfish Immunology
Volume5
Issue number3
DOIs
Publication statusPublished - Apr 1995

Fingerprint

Aeromonas salmonicida
antigen
rainbow
B-lymphocytes
Oncorhynchus mykiss
T-lymphocytes
Cells
Fish
antigens
Antigens
Blood
Iron
blood
leukocytes
iron
Bacteria
fish
cells
B-Lymphocyte Epitopes
bacterium

Keywords

  • Aeromonas salmonicida
  • B (sIg) cells
  • proliferation
  • Rainbow trout
  • T (sIg) cells

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

Cite this

Proliferative responses of rainbow trout, Oncorhynchusmykiss, T and B cells to antigens of Aeromonas salmonicida. / Marsden, M. J.; Hamdani, S. H.; Secombes, C. J.

In: Fish and Shellfish Immunology, Vol. 5, No. 3, 04.1995, p. 199-210.

Research output: Contribution to journalArticle

Marsden, M. J. ; Hamdani, S. H. ; Secombes, C. J. / Proliferative responses of rainbow trout, Oncorhynchusmykiss, T and B cells to antigens of Aeromonas salmonicida. In: Fish and Shellfish Immunology. 1995 ; Vol. 5, No. 3. pp. 199-210.
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