Proposal of Roseburia faecis sp nov., Roseburia hominis sp nov and Roseburia inulinivorans sp nov., based on isolates from human faeces

Sylvia Helen Duncan, Rustam Irekovitch Aminov, Karen Patricia Scott, Petra Louis, Thaddeus B. Stanton, Harry James Flint

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102 Citations (Scopus)

Abstract

Seven recently cultured bacterial isolates, although similar in their 16S rRNA gene sequences to Roseburia intestinalis L1-82(T) (DSM 14610(T)), Were not sufficiently related for inclusion within existing species, forming three separate clusters in a 16S rRNA gene phylogenetic tree. The isolates, which were obtained from human stools, were Gram-variable or Gram-negative, strictly anaerobic, slightly curved rods; cells from all strains measured approximately 0.5 x 1.5-5.0 mu m and were motile. Two strains belonging to one cluster (A2-181 and A2-183(T)) were the only strains that were able to grow on glycerol and that failed to grow on any of the complex substrates tested (inulin, xylan and amylopectin). Strains belonging to a second cluster (represented by M6/1 and M72/1(T)) differed from the other isolates in their ability to grow on sorbitol. Isolates belonging to a third cluster (L1-83 and A2-194(T)) were the only strains that failed to grow on xylose and that gave good growth on inulin (strains M6/1 and M72/1(T) gave weak growth). All strains were net acetate utilizers. The DNA G + C contents of representative Roseburia strains A2-183(T), A2-194(T), M72/1(T) and R. intestinalis L1-82(T) were 47.4, 41.4, 42.0 and 42.6 mol%, respectively. Based on 16S rRNA gene sequence similarity, three novel Roseburia species are proposed, with the names Roseburia hominis sp. nov. (type strain A2-183(T) = DSM 16839(T) = NCIMB 14029(T)), Roseburia inulinivorans sp. nov. (type strain A2-194(T) = DSM 16841(T) = NCIMB 14030(T)) and Roseburia faecis sp. nov. (type strain M72/1(T) = DSM 16840(T) = NCIMB 14031(T)).

Original languageEnglish
Pages (from-to)2437-2441
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume56
Issue number10
DOIs
Publication statusPublished - Oct 2006

Keywords

  • butyrate-producing bacteria
  • performance liquid-chromatography
  • gut

Cite this

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title = "Proposal of Roseburia faecis sp nov., Roseburia hominis sp nov and Roseburia inulinivorans sp nov., based on isolates from human faeces",
abstract = "Seven recently cultured bacterial isolates, although similar in their 16S rRNA gene sequences to Roseburia intestinalis L1-82(T) (DSM 14610(T)), Were not sufficiently related for inclusion within existing species, forming three separate clusters in a 16S rRNA gene phylogenetic tree. The isolates, which were obtained from human stools, were Gram-variable or Gram-negative, strictly anaerobic, slightly curved rods; cells from all strains measured approximately 0.5 x 1.5-5.0 mu m and were motile. Two strains belonging to one cluster (A2-181 and A2-183(T)) were the only strains that were able to grow on glycerol and that failed to grow on any of the complex substrates tested (inulin, xylan and amylopectin). Strains belonging to a second cluster (represented by M6/1 and M72/1(T)) differed from the other isolates in their ability to grow on sorbitol. Isolates belonging to a third cluster (L1-83 and A2-194(T)) were the only strains that failed to grow on xylose and that gave good growth on inulin (strains M6/1 and M72/1(T) gave weak growth). All strains were net acetate utilizers. The DNA G + C contents of representative Roseburia strains A2-183(T), A2-194(T), M72/1(T) and R. intestinalis L1-82(T) were 47.4, 41.4, 42.0 and 42.6 mol{\%}, respectively. Based on 16S rRNA gene sequence similarity, three novel Roseburia species are proposed, with the names Roseburia hominis sp. nov. (type strain A2-183(T) = DSM 16839(T) = NCIMB 14029(T)), Roseburia inulinivorans sp. nov. (type strain A2-194(T) = DSM 16841(T) = NCIMB 14030(T)) and Roseburia faecis sp. nov. (type strain M72/1(T) = DSM 16840(T) = NCIMB 14031(T)).",
keywords = "butyrate-producing bacteria, performance liquid-chromatography, gut",
author = "Duncan, {Sylvia Helen} and Aminov, {Rustam Irekovitch} and Scott, {Karen Patricia} and Petra Louis and Stanton, {Thaddeus B.} and Flint, {Harry James}",
year = "2006",
month = "10",
doi = "10.1099/ijs.0.64098-0",
language = "English",
volume = "56",
pages = "2437--2441",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "10",

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T1 - Proposal of Roseburia faecis sp nov., Roseburia hominis sp nov and Roseburia inulinivorans sp nov., based on isolates from human faeces

AU - Duncan, Sylvia Helen

AU - Aminov, Rustam Irekovitch

AU - Scott, Karen Patricia

AU - Louis, Petra

AU - Stanton, Thaddeus B.

AU - Flint, Harry James

PY - 2006/10

Y1 - 2006/10

N2 - Seven recently cultured bacterial isolates, although similar in their 16S rRNA gene sequences to Roseburia intestinalis L1-82(T) (DSM 14610(T)), Were not sufficiently related for inclusion within existing species, forming three separate clusters in a 16S rRNA gene phylogenetic tree. The isolates, which were obtained from human stools, were Gram-variable or Gram-negative, strictly anaerobic, slightly curved rods; cells from all strains measured approximately 0.5 x 1.5-5.0 mu m and were motile. Two strains belonging to one cluster (A2-181 and A2-183(T)) were the only strains that were able to grow on glycerol and that failed to grow on any of the complex substrates tested (inulin, xylan and amylopectin). Strains belonging to a second cluster (represented by M6/1 and M72/1(T)) differed from the other isolates in their ability to grow on sorbitol. Isolates belonging to a third cluster (L1-83 and A2-194(T)) were the only strains that failed to grow on xylose and that gave good growth on inulin (strains M6/1 and M72/1(T) gave weak growth). All strains were net acetate utilizers. The DNA G + C contents of representative Roseburia strains A2-183(T), A2-194(T), M72/1(T) and R. intestinalis L1-82(T) were 47.4, 41.4, 42.0 and 42.6 mol%, respectively. Based on 16S rRNA gene sequence similarity, three novel Roseburia species are proposed, with the names Roseburia hominis sp. nov. (type strain A2-183(T) = DSM 16839(T) = NCIMB 14029(T)), Roseburia inulinivorans sp. nov. (type strain A2-194(T) = DSM 16841(T) = NCIMB 14030(T)) and Roseburia faecis sp. nov. (type strain M72/1(T) = DSM 16840(T) = NCIMB 14031(T)).

AB - Seven recently cultured bacterial isolates, although similar in their 16S rRNA gene sequences to Roseburia intestinalis L1-82(T) (DSM 14610(T)), Were not sufficiently related for inclusion within existing species, forming three separate clusters in a 16S rRNA gene phylogenetic tree. The isolates, which were obtained from human stools, were Gram-variable or Gram-negative, strictly anaerobic, slightly curved rods; cells from all strains measured approximately 0.5 x 1.5-5.0 mu m and were motile. Two strains belonging to one cluster (A2-181 and A2-183(T)) were the only strains that were able to grow on glycerol and that failed to grow on any of the complex substrates tested (inulin, xylan and amylopectin). Strains belonging to a second cluster (represented by M6/1 and M72/1(T)) differed from the other isolates in their ability to grow on sorbitol. Isolates belonging to a third cluster (L1-83 and A2-194(T)) were the only strains that failed to grow on xylose and that gave good growth on inulin (strains M6/1 and M72/1(T) gave weak growth). All strains were net acetate utilizers. The DNA G + C contents of representative Roseburia strains A2-183(T), A2-194(T), M72/1(T) and R. intestinalis L1-82(T) were 47.4, 41.4, 42.0 and 42.6 mol%, respectively. Based on 16S rRNA gene sequence similarity, three novel Roseburia species are proposed, with the names Roseburia hominis sp. nov. (type strain A2-183(T) = DSM 16839(T) = NCIMB 14029(T)), Roseburia inulinivorans sp. nov. (type strain A2-194(T) = DSM 16841(T) = NCIMB 14030(T)) and Roseburia faecis sp. nov. (type strain M72/1(T) = DSM 16840(T) = NCIMB 14031(T)).

KW - butyrate-producing bacteria

KW - performance liquid-chromatography

KW - gut

U2 - 10.1099/ijs.0.64098-0

DO - 10.1099/ijs.0.64098-0

M3 - Article

VL - 56

SP - 2437

EP - 2441

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 10

ER -