TY - JOUR
T1 - Proposal of Roseburia faecis sp nov., Roseburia hominis sp nov and Roseburia inulinivorans sp nov., based on isolates from human faeces
AU - Duncan, Sylvia Helen
AU - Aminov, Rustam Irekovitch
AU - Scott, Karen Patricia
AU - Louis, Petra
AU - Stanton, Thaddeus B.
AU - Flint, Harry James
PY - 2006/10
Y1 - 2006/10
N2 - Seven recently cultured bacterial isolates, although similar in their 16S rRNA gene sequences to Roseburia intestinalis L1-82(T) (DSM 14610(T)), Were not sufficiently related for inclusion within existing species, forming three separate clusters in a 16S rRNA gene phylogenetic tree. The isolates, which were obtained from human stools, were Gram-variable or Gram-negative, strictly anaerobic, slightly curved rods; cells from all strains measured approximately 0.5 x 1.5-5.0 mu m and were motile. Two strains belonging to one cluster (A2-181 and A2-183(T)) were the only strains that were able to grow on glycerol and that failed to grow on any of the complex substrates tested (inulin, xylan and amylopectin). Strains belonging to a second cluster (represented by M6/1 and M72/1(T)) differed from the other isolates in their ability to grow on sorbitol. Isolates belonging to a third cluster (L1-83 and A2-194(T)) were the only strains that failed to grow on xylose and that gave good growth on inulin (strains M6/1 and M72/1(T) gave weak growth). All strains were net acetate utilizers. The DNA G + C contents of representative Roseburia strains A2-183(T), A2-194(T), M72/1(T) and R. intestinalis L1-82(T) were 47.4, 41.4, 42.0 and 42.6 mol%, respectively. Based on 16S rRNA gene sequence similarity, three novel Roseburia species are proposed, with the names Roseburia hominis sp. nov. (type strain A2-183(T) = DSM 16839(T) = NCIMB 14029(T)), Roseburia inulinivorans sp. nov. (type strain A2-194(T) = DSM 16841(T) = NCIMB 14030(T)) and Roseburia faecis sp. nov. (type strain M72/1(T) = DSM 16840(T) = NCIMB 14031(T)).
AB - Seven recently cultured bacterial isolates, although similar in their 16S rRNA gene sequences to Roseburia intestinalis L1-82(T) (DSM 14610(T)), Were not sufficiently related for inclusion within existing species, forming three separate clusters in a 16S rRNA gene phylogenetic tree. The isolates, which were obtained from human stools, were Gram-variable or Gram-negative, strictly anaerobic, slightly curved rods; cells from all strains measured approximately 0.5 x 1.5-5.0 mu m and were motile. Two strains belonging to one cluster (A2-181 and A2-183(T)) were the only strains that were able to grow on glycerol and that failed to grow on any of the complex substrates tested (inulin, xylan and amylopectin). Strains belonging to a second cluster (represented by M6/1 and M72/1(T)) differed from the other isolates in their ability to grow on sorbitol. Isolates belonging to a third cluster (L1-83 and A2-194(T)) were the only strains that failed to grow on xylose and that gave good growth on inulin (strains M6/1 and M72/1(T) gave weak growth). All strains were net acetate utilizers. The DNA G + C contents of representative Roseburia strains A2-183(T), A2-194(T), M72/1(T) and R. intestinalis L1-82(T) were 47.4, 41.4, 42.0 and 42.6 mol%, respectively. Based on 16S rRNA gene sequence similarity, three novel Roseburia species are proposed, with the names Roseburia hominis sp. nov. (type strain A2-183(T) = DSM 16839(T) = NCIMB 14029(T)), Roseburia inulinivorans sp. nov. (type strain A2-194(T) = DSM 16841(T) = NCIMB 14030(T)) and Roseburia faecis sp. nov. (type strain M72/1(T) = DSM 16840(T) = NCIMB 14031(T)).
KW - butyrate-producing bacteria
KW - performance liquid-chromatography
KW - gut
U2 - 10.1099/ijs.0.64098-0
DO - 10.1099/ijs.0.64098-0
M3 - Article
SN - 1466-5026
VL - 56
SP - 2437
EP - 2441
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 10
ER -