Prospective purification of a subpopulation of human synovial mesenchymal stem cells with enhanced chondro-osteogenic potency.

Francesca Gullo*, Cosimo De Bari

*Corresponding author for this work

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We previously reported the coexistence, within cultured mesenchymal stem cells (MSCs) from human synovial membrane, of single-cell-derived clonal cell populations with distinct differentiation potency. The aim of this study was to investigate marker sets for prospective purification of functionally distinct MSC subsets. Cells were enzymatically released from human synovium and culture expanded. Phenotype analysis was performed by flow cytometry using combinations of MSC markers. Sorting was carried out using the FACS DiVA cell sorter. Sorted cell populations were assessed for clonogenicity, kinetics of growth, cell senescence and chondro-osteogenic potency. During culture expansion, the co-localization of CD39 within the CD73(+) cell population identified a small cell subset that was maintained from passage 1 (P1) up to at least P12 in all donors tested. The CD73(+)CD39(+) cell subset displayed higher expression levels of Sox9 and Runx2 and a significantly greater chondro-osteogenic potency than the CD73(+)CD39(-) cell subset. In contrast, it was less clonogenic and proliferative. There was no difference in cell senescence between the sorted MSC subsets and the parental MSCs. Notably, there were no detectable differences in chondro-osteogenic potency between the CD73(+)CD39(-) and CD73(+)CD39(+) cell subsets purified from fresh synovial cell populations. Our findings indicate that the combination of CD73 and CD39 allows the prospective purification from culture-expanded heterogeneous synovial MSC populations of a distinct MSC subset with greater chondro-osteogenic potency. We anticipate that such an approach will enhance the consistency of cell-based therapeutic protocols for the repair of osteochondral defects.

Original languageEnglish
Pages (from-to)1758-1768
Number of pages11
JournalRheumatology
Volume52
Issue number10
Early online date26 Jun 2013
DOIs
Publication statusPublished - Oct 2013

Fingerprint

Mesenchymal Stromal Cells
Synovial Membrane
Population
Cell Aging
Flow Cytometry
Phenotype

Keywords

  • mesenchymal stem cells,
  • chondrogenesis
  • osteogenesis
  • osteoarthritis
  • synovium

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Prospective purification of a subpopulation of human synovial mesenchymal stem cells with enhanced chondro-osteogenic potency. / Gullo, Francesca; De Bari, Cosimo.

In: Rheumatology, Vol. 52, No. 10, 10.2013, p. 1758-1768.

Research output: Contribution to journalArticle

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abstract = "We previously reported the coexistence, within cultured mesenchymal stem cells (MSCs) from human synovial membrane, of single-cell-derived clonal cell populations with distinct differentiation potency. The aim of this study was to investigate marker sets for prospective purification of functionally distinct MSC subsets. Cells were enzymatically released from human synovium and culture expanded. Phenotype analysis was performed by flow cytometry using combinations of MSC markers. Sorting was carried out using the FACS DiVA cell sorter. Sorted cell populations were assessed for clonogenicity, kinetics of growth, cell senescence and chondro-osteogenic potency. During culture expansion, the co-localization of CD39 within the CD73(+) cell population identified a small cell subset that was maintained from passage 1 (P1) up to at least P12 in all donors tested. The CD73(+)CD39(+) cell subset displayed higher expression levels of Sox9 and Runx2 and a significantly greater chondro-osteogenic potency than the CD73(+)CD39(-) cell subset. In contrast, it was less clonogenic and proliferative. There was no difference in cell senescence between the sorted MSC subsets and the parental MSCs. Notably, there were no detectable differences in chondro-osteogenic potency between the CD73(+)CD39(-) and CD73(+)CD39(+) cell subsets purified from fresh synovial cell populations. Our findings indicate that the combination of CD73 and CD39 allows the prospective purification from culture-expanded heterogeneous synovial MSC populations of a distinct MSC subset with greater chondro-osteogenic potency. We anticipate that such an approach will enhance the consistency of cell-based therapeutic protocols for the repair of osteochondral defects.",
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note = "Acknowledgements F.G. was supported by an Oliver Bird Studentship Programme from the Nuffield Foundation. We thank Professor Jimmy Hutchison and the Orthopaedic Department at the Aberdeen Royal Infirmary for the provision of human synovial tissue samples. We thank Dr Raif Yucel and the FACS facility of the University of Aberdeen for their advice and support. We are also grateful to the Musculoskeletal Research Programme for technical and infrastructural support and to the entire Regenerative Medicine Group for critical reviewing of the data. Funding: Supported by the Nuffield Foundation (grant RHE/00092/G 24105) and the Arthritis Research UK Tissue Engineering Centre (grant 19429). Disclosure statement: The authors have declared no conflicts of interest.",
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