PROTECTION OF NITROSOMONAS-EUROPAEA COLONIZING CLAY-MINERALS FROM INHIBITION BY NITRAPYRIN

Judith Anne Powell, James Ivor Prosser

Research output: Contribution to journalArticle

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Abstract

Nitrite production by Nitrosomonas europaea in inorganic liquid medium containing ammonium was limited by reduction in pH. In the presence of montmorillonite and vermiculite, expanding clays with high cation-exchange-capacity (CEC), nitrite yield was increased, ammonia oxidation continued at pH values below those which inhibited growth in the absence of clays and growth was biphasic. The first phase was similar to that in the absence of clays, while the second was characterized by a lower rate of nitrite production. Illite, a non-expanding clay with low CEC, had no significant effect on ammonia oxidation, while oxidation of ammonia-treated vermiculite (ATV) occurred with no significant change in the pH of the medium. ATV, montmorillonite and vermiculite, but not illite, protected cells from inhibition by nitrapyrin at concentrations inhibitory to cells growing in suspended culture. This protection was maintained in ATV homo-ionic to Al3+, but montmorillonite made homo-ionic to Al3+ did not provide protection from inhibition. Attachment of cells to clays with high CEC is therefore advantageous in providing exchange at the clay surface of NH4+ and H+ produced by ammonia oxidation, in reducing pH toxicity, and in protecting cells from inhibition.

Original languageEnglish
Pages (from-to)1923-1929
Number of pages7
JournalAntonie van Leeuwenhoek
Volume137
Publication statusPublished - Aug 1991

Keywords

  • POTASSIUM ETHYL XANTHATE
  • LIQUID CULTURE
  • NITRIFICATION
  • SOIL
  • BACTERIA
  • GROWTH

Cite this

PROTECTION OF NITROSOMONAS-EUROPAEA COLONIZING CLAY-MINERALS FROM INHIBITION BY NITRAPYRIN. / Powell, Judith Anne; Prosser, James Ivor.

In: Antonie van Leeuwenhoek , Vol. 137, 08.1991, p. 1923-1929.

Research output: Contribution to journalArticle

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abstract = "Nitrite production by Nitrosomonas europaea in inorganic liquid medium containing ammonium was limited by reduction in pH. In the presence of montmorillonite and vermiculite, expanding clays with high cation-exchange-capacity (CEC), nitrite yield was increased, ammonia oxidation continued at pH values below those which inhibited growth in the absence of clays and growth was biphasic. The first phase was similar to that in the absence of clays, while the second was characterized by a lower rate of nitrite production. Illite, a non-expanding clay with low CEC, had no significant effect on ammonia oxidation, while oxidation of ammonia-treated vermiculite (ATV) occurred with no significant change in the pH of the medium. ATV, montmorillonite and vermiculite, but not illite, protected cells from inhibition by nitrapyrin at concentrations inhibitory to cells growing in suspended culture. This protection was maintained in ATV homo-ionic to Al3+, but montmorillonite made homo-ionic to Al3+ did not provide protection from inhibition. Attachment of cells to clays with high CEC is therefore advantageous in providing exchange at the clay surface of NH4+ and H+ produced by ammonia oxidation, in reducing pH toxicity, and in protecting cells from inhibition.",
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T1 - PROTECTION OF NITROSOMONAS-EUROPAEA COLONIZING CLAY-MINERALS FROM INHIBITION BY NITRAPYRIN

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AU - Prosser, James Ivor

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N2 - Nitrite production by Nitrosomonas europaea in inorganic liquid medium containing ammonium was limited by reduction in pH. In the presence of montmorillonite and vermiculite, expanding clays with high cation-exchange-capacity (CEC), nitrite yield was increased, ammonia oxidation continued at pH values below those which inhibited growth in the absence of clays and growth was biphasic. The first phase was similar to that in the absence of clays, while the second was characterized by a lower rate of nitrite production. Illite, a non-expanding clay with low CEC, had no significant effect on ammonia oxidation, while oxidation of ammonia-treated vermiculite (ATV) occurred with no significant change in the pH of the medium. ATV, montmorillonite and vermiculite, but not illite, protected cells from inhibition by nitrapyrin at concentrations inhibitory to cells growing in suspended culture. This protection was maintained in ATV homo-ionic to Al3+, but montmorillonite made homo-ionic to Al3+ did not provide protection from inhibition. Attachment of cells to clays with high CEC is therefore advantageous in providing exchange at the clay surface of NH4+ and H+ produced by ammonia oxidation, in reducing pH toxicity, and in protecting cells from inhibition.

AB - Nitrite production by Nitrosomonas europaea in inorganic liquid medium containing ammonium was limited by reduction in pH. In the presence of montmorillonite and vermiculite, expanding clays with high cation-exchange-capacity (CEC), nitrite yield was increased, ammonia oxidation continued at pH values below those which inhibited growth in the absence of clays and growth was biphasic. The first phase was similar to that in the absence of clays, while the second was characterized by a lower rate of nitrite production. Illite, a non-expanding clay with low CEC, had no significant effect on ammonia oxidation, while oxidation of ammonia-treated vermiculite (ATV) occurred with no significant change in the pH of the medium. ATV, montmorillonite and vermiculite, but not illite, protected cells from inhibition by nitrapyrin at concentrations inhibitory to cells growing in suspended culture. This protection was maintained in ATV homo-ionic to Al3+, but montmorillonite made homo-ionic to Al3+ did not provide protection from inhibition. Attachment of cells to clays with high CEC is therefore advantageous in providing exchange at the clay surface of NH4+ and H+ produced by ammonia oxidation, in reducing pH toxicity, and in protecting cells from inhibition.

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KW - LIQUID CULTURE

KW - NITRIFICATION

KW - SOIL

KW - BACTERIA

KW - GROWTH

M3 - Article

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JO - Antonie van Leeuwenhoek

JF - Antonie van Leeuwenhoek

SN - 0003-6072

ER -