Protection of the DNA during the exposure of Escherichia coli cells to a toxic metabolite: the role of the KefB and KefC potassium channels

G P Ferguson, J R Battista, A T Lee, I R Booth

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The effect of the toxic metabolite methylglyoxal on the DNA of Escherichia coil cells has been investigated. Exposure of E. coli cells to methylglyoxal reduces the transformability of plasmid DNA and results in the degradation of genomic DNA. The activity of the KefB and KefC potassium channels protects E. coil cells against methylglyoxal and limits the amount of DNA damage. In mutants lacking KefB and KefC, methylglyoxal-induced DNA damage was reduced by incubation with a weak acid that lowers the pi-ii to the same extent as through KefB and KefC activation. This provides evidence that acidification of the cytoplasm protects E. coil DNA against methylglyoxal. By the analysis of cells lacking UvrA, we demonstrate that this repair protein is required for the degradation of the DNA upon methylglyoxal exposure. However, protection by KefB and KefC occurred independently of UvrA. Although we present evidence that exposure of E. coli cells to methylglyoxal results in DNA degradation, our results suggest this event is not essential for methylglyoxal-induced death. The implications of these findings will be discussed.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalMolecular Microbiology
Volume35
Publication statusPublished - 2000

Keywords

  • METHYLGLYOXAL PRODUCTION
  • N-ETHYLMALEIMIDE
  • GLUTATHIONE
  • K-12
  • K+
  • DETOXIFICATION
  • ACTIVATION
  • SURVIVAL
  • N-METHYL-N'-NITRO-N-NITROSOGUANIDINE
  • MICROORGANISMS

Cite this

Protection of the DNA during the exposure of Escherichia coli cells to a toxic metabolite: the role of the KefB and KefC potassium channels. / Ferguson, G P ; Battista, J R ; Lee, A T ; Booth, I R .

In: Molecular Microbiology, Vol. 35, 2000, p. 113-122.

Research output: Contribution to journalArticle

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abstract = "The effect of the toxic metabolite methylglyoxal on the DNA of Escherichia coil cells has been investigated. Exposure of E. coli cells to methylglyoxal reduces the transformability of plasmid DNA and results in the degradation of genomic DNA. The activity of the KefB and KefC potassium channels protects E. coil cells against methylglyoxal and limits the amount of DNA damage. In mutants lacking KefB and KefC, methylglyoxal-induced DNA damage was reduced by incubation with a weak acid that lowers the pi-ii to the same extent as through KefB and KefC activation. This provides evidence that acidification of the cytoplasm protects E. coil DNA against methylglyoxal. By the analysis of cells lacking UvrA, we demonstrate that this repair protein is required for the degradation of the DNA upon methylglyoxal exposure. However, protection by KefB and KefC occurred independently of UvrA. Although we present evidence that exposure of E. coli cells to methylglyoxal results in DNA degradation, our results suggest this event is not essential for methylglyoxal-induced death. The implications of these findings will be discussed.",
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T1 - Protection of the DNA during the exposure of Escherichia coli cells to a toxic metabolite: the role of the KefB and KefC potassium channels

AU - Ferguson, G P

AU - Battista, J R

AU - Lee, A T

AU - Booth, I R

PY - 2000

Y1 - 2000

N2 - The effect of the toxic metabolite methylglyoxal on the DNA of Escherichia coil cells has been investigated. Exposure of E. coli cells to methylglyoxal reduces the transformability of plasmid DNA and results in the degradation of genomic DNA. The activity of the KefB and KefC potassium channels protects E. coil cells against methylglyoxal and limits the amount of DNA damage. In mutants lacking KefB and KefC, methylglyoxal-induced DNA damage was reduced by incubation with a weak acid that lowers the pi-ii to the same extent as through KefB and KefC activation. This provides evidence that acidification of the cytoplasm protects E. coil DNA against methylglyoxal. By the analysis of cells lacking UvrA, we demonstrate that this repair protein is required for the degradation of the DNA upon methylglyoxal exposure. However, protection by KefB and KefC occurred independently of UvrA. Although we present evidence that exposure of E. coli cells to methylglyoxal results in DNA degradation, our results suggest this event is not essential for methylglyoxal-induced death. The implications of these findings will be discussed.

AB - The effect of the toxic metabolite methylglyoxal on the DNA of Escherichia coil cells has been investigated. Exposure of E. coli cells to methylglyoxal reduces the transformability of plasmid DNA and results in the degradation of genomic DNA. The activity of the KefB and KefC potassium channels protects E. coil cells against methylglyoxal and limits the amount of DNA damage. In mutants lacking KefB and KefC, methylglyoxal-induced DNA damage was reduced by incubation with a weak acid that lowers the pi-ii to the same extent as through KefB and KefC activation. This provides evidence that acidification of the cytoplasm protects E. coil DNA against methylglyoxal. By the analysis of cells lacking UvrA, we demonstrate that this repair protein is required for the degradation of the DNA upon methylglyoxal exposure. However, protection by KefB and KefC occurred independently of UvrA. Although we present evidence that exposure of E. coli cells to methylglyoxal results in DNA degradation, our results suggest this event is not essential for methylglyoxal-induced death. The implications of these findings will be discussed.

KW - METHYLGLYOXAL PRODUCTION

KW - N-ETHYLMALEIMIDE

KW - GLUTATHIONE

KW - K-12

KW - K+

KW - DETOXIFICATION

KW - ACTIVATION

KW - SURVIVAL

KW - N-METHYL-N'-NITRO-N-NITROSOGUANIDINE

KW - MICROORGANISMS

M3 - Article

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EP - 122

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

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