Protein growth performance, amino acid utilisation and somatotropic axis responsiveness to fish meal replacement by plant protein sources in gilthead sea bream (Sparus aurata)

P. Gomez-Requeni, M. Mingarro, J. A. Calduch-Giner, F. Medale, Samuel Allen Moore Martin, Dominic Francis Joseph Houlihan, S. J. Kaushik, J. Perez-Sanchez

Research output: Contribution to journalArticle

262 Citations (Scopus)

Abstract

Partial or total replacement of fish meal by a mixture of plant protein sources (corn gluten meal, wheat gluten, extruded peas, rapeseed meal) balanced with indispensable amino acids (IAA) was examined in juvenile gilthead sea bream (Sparus aurata) over the course of a 12-week growth trial. A diet with fish meal (FM) as the sole protein source was compared to diets with 50%, 75% and 100% of replacement (PP50, PP75, PP100). Protein retention was improved with more plant protein supply, and just a slight decrease in the final weight gain was found in fish fed PP50 and PP75 diets. However, in the PP100 group, weight gain was depressed up to 30% mainly as the result of a marked reduction of feed intake. These fish also showed a lower fat gain along with a marked hypocholesterolemic effect. Dietary treatment did not alter the hepatic activity of amino acid catabolising enzymes (alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), glutamate dehydrogenase (GDH)), although the size of the total muscle free amino acid (FAA) pool was increased by more plant protein supply. The activity of the somatotropic axis also varied among experimental groups, and the up-regulation of circulating growth hormone (GH) levels with a high plant protein supply followed the decrease in growth rates, plasma levels of insulin-like growth factor-I (IGF-I), and liver mRNA transcripts of lGF-I and GH receptors. This catabolic feature evidenced a liver desensitisation to the anabolic action of GH in the PP100 group, and to a lesser extent in the PP75 group. Taken together all these findings, up to 50-75% of fish meal replacement seems to be feasible with IAA supplementation, but further research is needed to fully identify the responsible factors for the depressed feed intake in order to achieve a full replacement in a fish species having high dietary protein requirements. (C) 2004 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)493-510
Number of pages18
JournalAquaculture
Volume232
Issue number1-4
Early online date25 Sep 2003
DOIs
Publication statusPublished - 5 Apr 2004

Keywords

  • gilthead sea bream
  • plant protein
  • nutrient utilisation
  • free amino acids
  • growth hormone
  • growth hormone receptor
  • insulin-like growth factor-I
  • Trout oncorhynchus mykiss
  • Bass dicentrarchus labrax
  • Turbot psetta maxima
  • pea seed meal
  • Rainbow trout
  • factor-I
  • dietary protein
  • lysine supplementation
  • nutrient utilization
  • marine teleost

Cite this

Protein growth performance, amino acid utilisation and somatotropic axis responsiveness to fish meal replacement by plant protein sources in gilthead sea bream (Sparus aurata). / Gomez-Requeni, P.; Mingarro, M.; Calduch-Giner, J. A.; Medale, F.; Martin, Samuel Allen Moore; Houlihan, Dominic Francis Joseph; Kaushik, S. J.; Perez-Sanchez, J.

In: Aquaculture, Vol. 232, No. 1-4, 05.04.2004, p. 493-510.

Research output: Contribution to journalArticle

Gomez-Requeni, P. ; Mingarro, M. ; Calduch-Giner, J. A. ; Medale, F. ; Martin, Samuel Allen Moore ; Houlihan, Dominic Francis Joseph ; Kaushik, S. J. ; Perez-Sanchez, J. / Protein growth performance, amino acid utilisation and somatotropic axis responsiveness to fish meal replacement by plant protein sources in gilthead sea bream (Sparus aurata). In: Aquaculture. 2004 ; Vol. 232, No. 1-4. pp. 493-510.
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AU - Calduch-Giner, J. A.

AU - Medale, F.

AU - Martin, Samuel Allen Moore

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AB - Partial or total replacement of fish meal by a mixture of plant protein sources (corn gluten meal, wheat gluten, extruded peas, rapeseed meal) balanced with indispensable amino acids (IAA) was examined in juvenile gilthead sea bream (Sparus aurata) over the course of a 12-week growth trial. A diet with fish meal (FM) as the sole protein source was compared to diets with 50%, 75% and 100% of replacement (PP50, PP75, PP100). Protein retention was improved with more plant protein supply, and just a slight decrease in the final weight gain was found in fish fed PP50 and PP75 diets. However, in the PP100 group, weight gain was depressed up to 30% mainly as the result of a marked reduction of feed intake. These fish also showed a lower fat gain along with a marked hypocholesterolemic effect. Dietary treatment did not alter the hepatic activity of amino acid catabolising enzymes (alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), glutamate dehydrogenase (GDH)), although the size of the total muscle free amino acid (FAA) pool was increased by more plant protein supply. The activity of the somatotropic axis also varied among experimental groups, and the up-regulation of circulating growth hormone (GH) levels with a high plant protein supply followed the decrease in growth rates, plasma levels of insulin-like growth factor-I (IGF-I), and liver mRNA transcripts of lGF-I and GH receptors. This catabolic feature evidenced a liver desensitisation to the anabolic action of GH in the PP100 group, and to a lesser extent in the PP75 group. Taken together all these findings, up to 50-75% of fish meal replacement seems to be feasible with IAA supplementation, but further research is needed to fully identify the responsible factors for the depressed feed intake in order to achieve a full replacement in a fish species having high dietary protein requirements. (C) 2004 Elsevier B.V. All rights reserved.

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KW - nutrient utilisation

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KW - growth hormone receptor

KW - insulin-like growth factor-I

KW - Trout oncorhynchus mykiss

KW - Bass dicentrarchus labrax

KW - Turbot psetta maxima

KW - pea seed meal

KW - Rainbow trout

KW - factor-I

KW - dietary protein

KW - lysine supplementation

KW - nutrient utilization

KW - marine teleost

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DO - 10.1016/S0044-8486(03)00532-5

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JO - Aquaculture

JF - Aquaculture

SN - 0044-8486

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ER -