Protein growth rate in rainbow trout (Oncorhynchus mykiss) is negatively correlated to liver 20S proteasome activity

A. Dobly, Samuel Allen Moore Martin, Susan Corral Blaney, Dominic Francis Joseph Houlihan

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The efficiency with which fish and other animals add and maintain body proteins is a balance between synthesis of proteins and their degradation. In fish that have similar food consumption and protein synthesis rates, a greater ratio of synthesis to degradation would be expected to produce more efficient conversion of food into growth. In addition, we hypothesised that high activities of the proteasome, a major pathway of protein degradation, would be negatively correlated with growth rate. In order to test this hypothesis we maintained rainbow trout for 62 days, during which repeat measurements of food consumption and growth were made. We selected fish for high and low growth efficiencies. Protein degradation was estimated from the difference between protein synthesis (determined by N flux) and protein growth. We found that protein synthesis rates were significantly higher in the low growth efficiency group, as were estimated protein degradation rates. In another group of fish that also did not differ in food consumption, the activity of the proteasome in the liver, but not in the muscle, was negatively correlated with growth rates. These two experiments showed that high proteasome activity is linked to decreased growth efficiency.
Original languageEnglish
Pages (from-to)75-85
Number of pages11
JournalComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
Volume137
Issue number1
DOIs
Publication statusPublished - 1 Jan 2004

Keywords

  • efficiency of protein conversion
  • growth
  • Oncorhynchus mykiss
  • proteasome
  • protein synthesis
  • protein degradation
  • protein turnover
  • rainbow trout
  • Salvelinus alpinus
  • stable isotope
  • Arctic charr
  • nitrogen excretion
  • food acquisition
  • term starvation
  • Gadus morhua
  • turnover
  • muscle
  • fish

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