Protein-Synthesis in Splanching Tissues of Sheep Offered 2 Levels of Intake

Gerald Lobley, A CONNELL, Eric Milne, T A EWING

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Protein synthesis rates were measured in Liver and gastrointestinal tract (GIT) sections of fattening sheep offered lucerne (Medicago sativa) pellets at either 1.25 or 2 times energy maintenance. The measurement technique involved a large dose of [1-C-13]valine over 60 min. Animals on the higher intake had a larger mass of liver protein (143 v. 100 g, P = 0.02), similar fractional synthesis rates (k(s); 22.5 v. 22.1%/d, not significant) and greater absolute amounts of protein synthesis (32 v. 23 g/d; P = 0.016) compared with those on the smaller amount of ration. The k(s) values and RNA:protein in the GIT sections also tended to increase with food intake. Estimated total GIT protein synthesis was approximately three-fold that in Liver and probably constituted 25-35% of whole body synthesis. All splanchnic tissues measured hsd lower translational efficiencies (g protein synthesized/d per g total RNA) than reported for milk-fed and newly-weaned lambs and this may relate to the decline in the rate of protein deposition as lambs progress to the fattening condition.

Original languageEnglish
Pages (from-to)3-12
Number of pages10
JournalBritish Journal of Nutrition
Volume71
Issue number1
DOIs
Publication statusPublished - Jan 1994

Keywords

  • liver
  • gastrointestinal
  • translational efficiency
  • sheep
  • whole-body
  • skeletal-muscle
  • intravenous-infusion
  • heat-production
  • growing lambs
  • metabolism
  • growth
  • insulin
  • leucine
  • rat

Cite this

Protein-Synthesis in Splanching Tissues of Sheep Offered 2 Levels of Intake. / Lobley, Gerald; CONNELL, A ; Milne, Eric; EWING, T A .

In: British Journal of Nutrition, Vol. 71, No. 1, 01.1994, p. 3-12.

Research output: Contribution to journalArticle

Lobley, Gerald ; CONNELL, A ; Milne, Eric ; EWING, T A . / Protein-Synthesis in Splanching Tissues of Sheep Offered 2 Levels of Intake. In: British Journal of Nutrition. 1994 ; Vol. 71, No. 1. pp. 3-12.
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