Protein turnover, amino acid profile and amino acid flux in juvenile shrimp Litopenaeus vannamei: effects of dietary protein source

Elena Mente, P. Coutteau, Dominic Francis Joseph Houlihan, Ian Davidson, P. Sorgeloos

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

The effect of dietary protein on protein synthesis and growth of juvenile shrimps Litopenaeus vannamei was investigated using three different diets with equivalent protein content. Protein synthesis was investigated by a flooding dose of tritiated phenylalanine. Survival, specific growth and protein synthesis rates were higher, and protein degradation was lower, in shrimps fed a fish/squid/shrimp meal diet, or a 50% laboratory diet/50% soybean meal variant diet, than in those fed a casein-based diet. The efficiency of retention of synthesized protein as growth was 94% for shrimps fed the fish meal diet, suggesting a very low protein turnover rate; by contrast, the retention of synthesized protein was only 80 for shrimps fed the casein diet. The amino acid profile of the casein diet was poorly correlated with that of the shrimps. 4 h after a single meal the protein synthesis rates increased following an increase in RNA activity. A model was developed for amino acid flux, suggesting that high growth rates involve a reduction in the turnover of proteins, while amino acid loss appears to be high.

Original languageEnglish
Pages (from-to)3107-3122
Number of pages15
JournalJournal of Experimental Biology
Volume205
Issue number20
Publication statusPublished - Oct 2002

Keywords

  • shrimp
  • Litopenaeus vannamei
  • growth
  • protein synthesis
  • diet
  • amino acid flux
  • casein
  • protein turnover
  • YOLK-SAC LARVAE
  • SKELETAL-MUSCLE
  • GADUS-MORHUA
  • ATLANTIC COD
  • OXYGEN-CONSUMPTION
  • FOOD-CONSUMPTION
  • SYNTHESIS RATES
  • RAINBOW-TROUT
  • TIGER PRAWN
  • WHOLE-BODY

Cite this

Protein turnover, amino acid profile and amino acid flux in juvenile shrimp Litopenaeus vannamei: effects of dietary protein source. / Mente, Elena; Coutteau, P.; Houlihan, Dominic Francis Joseph; Davidson, Ian; Sorgeloos, P.

In: Journal of Experimental Biology, Vol. 205, No. 20, 10.2002, p. 3107-3122.

Research output: Contribution to journalArticle

Mente, Elena ; Coutteau, P. ; Houlihan, Dominic Francis Joseph ; Davidson, Ian ; Sorgeloos, P. / Protein turnover, amino acid profile and amino acid flux in juvenile shrimp Litopenaeus vannamei: effects of dietary protein source. In: Journal of Experimental Biology. 2002 ; Vol. 205, No. 20. pp. 3107-3122.
@article{1e6cca5643b24068a8ec9ce307998e29,
title = "Protein turnover, amino acid profile and amino acid flux in juvenile shrimp Litopenaeus vannamei: effects of dietary protein source",
abstract = "The effect of dietary protein on protein synthesis and growth of juvenile shrimps Litopenaeus vannamei was investigated using three different diets with equivalent protein content. Protein synthesis was investigated by a flooding dose of tritiated phenylalanine. Survival, specific growth and protein synthesis rates were higher, and protein degradation was lower, in shrimps fed a fish/squid/shrimp meal diet, or a 50{\%} laboratory diet/50{\%} soybean meal variant diet, than in those fed a casein-based diet. The efficiency of retention of synthesized protein as growth was 94{\%} for shrimps fed the fish meal diet, suggesting a very low protein turnover rate; by contrast, the retention of synthesized protein was only 80 for shrimps fed the casein diet. The amino acid profile of the casein diet was poorly correlated with that of the shrimps. 4 h after a single meal the protein synthesis rates increased following an increase in RNA activity. A model was developed for amino acid flux, suggesting that high growth rates involve a reduction in the turnover of proteins, while amino acid loss appears to be high.",
keywords = "shrimp, Litopenaeus vannamei, growth, protein synthesis, diet, amino acid flux, casein, protein turnover, YOLK-SAC LARVAE, SKELETAL-MUSCLE, GADUS-MORHUA, ATLANTIC COD, OXYGEN-CONSUMPTION, FOOD-CONSUMPTION, SYNTHESIS RATES, RAINBOW-TROUT, TIGER PRAWN, WHOLE-BODY",
author = "Elena Mente and P. Coutteau and Houlihan, {Dominic Francis Joseph} and Ian Davidson and P. Sorgeloos",
year = "2002",
month = "10",
language = "English",
volume = "205",
pages = "3107--3122",
journal = "Journal of Experimental Biology",
issn = "0022-0949",
publisher = "Company of Biologists Ltd",
number = "20",

}

TY - JOUR

T1 - Protein turnover, amino acid profile and amino acid flux in juvenile shrimp Litopenaeus vannamei: effects of dietary protein source

AU - Mente, Elena

AU - Coutteau, P.

AU - Houlihan, Dominic Francis Joseph

AU - Davidson, Ian

AU - Sorgeloos, P.

PY - 2002/10

Y1 - 2002/10

N2 - The effect of dietary protein on protein synthesis and growth of juvenile shrimps Litopenaeus vannamei was investigated using three different diets with equivalent protein content. Protein synthesis was investigated by a flooding dose of tritiated phenylalanine. Survival, specific growth and protein synthesis rates were higher, and protein degradation was lower, in shrimps fed a fish/squid/shrimp meal diet, or a 50% laboratory diet/50% soybean meal variant diet, than in those fed a casein-based diet. The efficiency of retention of synthesized protein as growth was 94% for shrimps fed the fish meal diet, suggesting a very low protein turnover rate; by contrast, the retention of synthesized protein was only 80 for shrimps fed the casein diet. The amino acid profile of the casein diet was poorly correlated with that of the shrimps. 4 h after a single meal the protein synthesis rates increased following an increase in RNA activity. A model was developed for amino acid flux, suggesting that high growth rates involve a reduction in the turnover of proteins, while amino acid loss appears to be high.

AB - The effect of dietary protein on protein synthesis and growth of juvenile shrimps Litopenaeus vannamei was investigated using three different diets with equivalent protein content. Protein synthesis was investigated by a flooding dose of tritiated phenylalanine. Survival, specific growth and protein synthesis rates were higher, and protein degradation was lower, in shrimps fed a fish/squid/shrimp meal diet, or a 50% laboratory diet/50% soybean meal variant diet, than in those fed a casein-based diet. The efficiency of retention of synthesized protein as growth was 94% for shrimps fed the fish meal diet, suggesting a very low protein turnover rate; by contrast, the retention of synthesized protein was only 80 for shrimps fed the casein diet. The amino acid profile of the casein diet was poorly correlated with that of the shrimps. 4 h after a single meal the protein synthesis rates increased following an increase in RNA activity. A model was developed for amino acid flux, suggesting that high growth rates involve a reduction in the turnover of proteins, while amino acid loss appears to be high.

KW - shrimp

KW - Litopenaeus vannamei

KW - growth

KW - protein synthesis

KW - diet

KW - amino acid flux

KW - casein

KW - protein turnover

KW - YOLK-SAC LARVAE

KW - SKELETAL-MUSCLE

KW - GADUS-MORHUA

KW - ATLANTIC COD

KW - OXYGEN-CONSUMPTION

KW - FOOD-CONSUMPTION

KW - SYNTHESIS RATES

KW - RAINBOW-TROUT

KW - TIGER PRAWN

KW - WHOLE-BODY

M3 - Article

VL - 205

SP - 3107

EP - 3122

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

IS - 20

ER -