Proteome analysis of the Atlantic salmon (Salmo salar) cell line SHK-1 following recombinant IFN-¿ stimulation

Samuel Allen Moore Martin, Bimal P. Mohanty, Phillip Cash, Dominic Francis Joseph Houlihan, Christopher John Secombes

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46 Citations (Scopus)

Abstract

Type II IFN exists as a single molecule (IFN-gamma) in contrast to type I IFN, of which there are a number of different forms. IFN-gamma is involved both directly and indirectly in antiviral activity, stimulation of bactericidal activity, antigen presentation and activation of macrophages. Recently IFN-gamma was cloned from a salmonid fish, the rainbow trout and a functional recombinant protein produced exhibited IFN-gamma activity. This recombinant IFN-gamma was used to stimulate an Atlantic salmon cell line, SHK-1, to monitor the changes in protein expression by proteomic analysis 24 h after stimulation compared to unstimulated control cells. An SHK-1 cell proteome map was developed and proteins altered in abundance by the IFN-gamma stimulation were identified. Under the analytical conditions used, 22 proteins were found to be altered in abundance, 15 increased and 7 decreased. Several proteins were excised from the gel and identified, following trypsin digestion and MALDI-MS/MS/LC-MS and database interrogation. Transcriptional analysis of five mRNAs encoding proteins increased in abundance by IFN-gamma in the proteome analysis was determined by real-time PCR. We assessed the correlation between gene expression and change in abundance of proteins for these genes.

Original languageEnglish
Pages (from-to)2275-2286
Number of pages12
JournalProteomics
Volume7
Issue number13
DOIs
Publication statusPublished - Jun 2007

Keywords

  • Atlantic salmon
  • gene expression
  • interferon-gamma
  • Mx
  • salmo salar
  • trout oncorhynchus-mykiss
  • rainbow-trout
  • expression analysis
  • channel catfish
  • gene-expression
  • messenger-RNA
  • head kidney
  • identification
  • cloning

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