Proteome-determined type-specific proteins of Mycobacterium avium subspecies paratuberculosis

Valerie Hughes, Alfredo Garcia-Sanchez, Stuart Smith, Kevin Mclean, Alex Lainson, Mintu Nath, Karen Stevenson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mycobacterium avium subspecies paratuberculosis (M. a. paratuberculosis) is a pathogen of ruminants, causing paratuberculosis (characterized by severe emaciation). The disease is endemic in many countries including the UK and places a severe economic burden on the global livestock industry. Two types of M. a. paratuberculosis can be classified by pulsed-field electrophoresis (I/III and II), which are phenotypically distinct and appear to have different host preferences. Proteomes of Type I and Type II M. a. paratuberculosis were analyzed by 2-D gel electrophoresis to determine if any significant differences existed between the subtypes. Seven different strains of Type I and 18 strains of Type II were analyzed and compared to detect type-specific differences. These 'type-specific' differences existed regardless of growth phase and were also exhibited in cells isolated directly from pathogenic lesions. Twenty-three spots predominated on the Type I profile, from which 17 proteins were identified. Twenty-one spots predominated on the Type II profile, from which 16 proteins were identified. None of the proteins identified as differentially represented on the profiles of Type I or Type II corresponded to open reading frames of the defining genomic regions as previously described for the Type I (sheep) and Type II (cattle). Sequence polymorphisms existing in Type I and II strains were identified in some open reading frames or regulatory regions of genes that correspond to proteins expressed in a type-specific fashion. The consequence of these is discussed in relation to protein expression and their impact on the type phenotype is discussed.

Original languageEnglish
Pages (from-to)153-62
Number of pages10
JournalVeterinary Microbiology
Volume158
Issue number1-2
Early online date7 Feb 2012
DOIs
Publication statusPublished - 6 Jul 2012

Fingerprint

Mycobacterium avium subsp. paratuberculosis
Mycobacterium avium
paratuberculosis
Proteome
proteome
Paratuberculosis
open reading frames
Proteins
proteins
Open Reading Frames
emaciation
Emaciation
livestock and meat industry
host preferences
pulsed-field gel electrophoresis
Endemic Diseases
lesions (animal)
gel electrophoresis
Nucleic Acid Regulatory Sequences
Electrophoresis, Gel, Two-Dimensional

Keywords

  • Animals
  • Bacterial Proteins/analysis
  • Cattle
  • Cattle Diseases/microbiology
  • Electrophoresis, Gel, Two-Dimensional
  • Mycobacterium avium subsp. paratuberculosis/classification
  • Paratuberculosis/microbiology
  • Proteome/analysis
  • Proteomics
  • Sheep
  • Sheep Diseases/microbiology
  • Transcriptome

Cite this

Hughes, V., Garcia-Sanchez, A., Smith, S., Mclean, K., Lainson, A., Nath, M., & Stevenson, K. (2012). Proteome-determined type-specific proteins of Mycobacterium avium subspecies paratuberculosis. Veterinary Microbiology, 158(1-2), 153-62. https://doi.org/10.1016/j.vetmic.2012.01.032

Proteome-determined type-specific proteins of Mycobacterium avium subspecies paratuberculosis. / Hughes, Valerie; Garcia-Sanchez, Alfredo; Smith, Stuart; Mclean, Kevin; Lainson, Alex; Nath, Mintu; Stevenson, Karen.

In: Veterinary Microbiology, Vol. 158, No. 1-2, 06.07.2012, p. 153-62.

Research output: Contribution to journalArticle

Hughes, V, Garcia-Sanchez, A, Smith, S, Mclean, K, Lainson, A, Nath, M & Stevenson, K 2012, 'Proteome-determined type-specific proteins of Mycobacterium avium subspecies paratuberculosis', Veterinary Microbiology, vol. 158, no. 1-2, pp. 153-62. https://doi.org/10.1016/j.vetmic.2012.01.032
Hughes, Valerie ; Garcia-Sanchez, Alfredo ; Smith, Stuart ; Mclean, Kevin ; Lainson, Alex ; Nath, Mintu ; Stevenson, Karen. / Proteome-determined type-specific proteins of Mycobacterium avium subspecies paratuberculosis. In: Veterinary Microbiology. 2012 ; Vol. 158, No. 1-2. pp. 153-62.
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