Proteomic analysis of the excretory-secretory proteins of the Trichinella spiralis L1 larva, a nematode parasite of skeletal muscle

Mark William Robinson, Bernadette Connolly

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72 Citations (Scopus)

Abstract

Trichinella spiralis is an intracellular nematode parasite of mammalian skeletal muscle. Infection of the muscle cell leads to the formation of a host-parasite complex that results in profound alterations to the host cell and a re-alignment of muscle-specific gene expression. The role of parasite excretory-secretory (ES) proteins in mediating these effects is currently unknown, largely due to the difficulty in identifying and assigning function to individual proteins. In this study, a global proteomics approach was used to analyse the ES proteins from T spiralis muscle larvae. Following 2-DE of ES proteins,MALDI-TOF-MS and LC-MS/MS were used to identify the peptide spots. Specific Trichinella EST databases were assembled and used to analyse the data. Despite the current absence of a Trichinella genome-sequencing project, 43 out of 52 protein spots analysed were identified and included the major secreted glycoproteins. Other novel proteins were identified from matches with sequences in the T spiralis database. Our results demonstrate the value of proteomics as a tool for the identification of Trichinella ES proteins and in the study of the molecular mechanism underpinning the formation of the host-parasite complex during Trichinella infections.

Original languageEnglish
Pages (from-to)4525-4532
Number of pages7
JournalProteomics
Volume5
Issue number17
DOIs
Publication statusPublished - Nov 2005

Keywords

  • MALDI-TOF
  • MS/MS
  • muscle larva
  • nematode
  • Trichinella
  • excretory/secretory products
  • endonuclease activity
  • molecular-cloning
  • fasciola-hepatica
  • nuclear antigens
  • rapid expulsion
  • in-vitro
  • cells
  • immunodominant
  • psuedospiralis

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