Proteomic response to amino acid starvation in Candida albicans and Saccharomyces cerevisiae

Zhikang Yin, David Andrew Stead, Laura Selway, Janet Walker, I. Riba-Garcia, T. McInerney, S. Gaskell, S. G. Oliver, Phillip Cash, Alistair James Petersen Brown

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Saccharomyces cerevisiae activates general amino acid control (GCN) in response to amino acid starvation. Some aspects of this response are known to be conserved in other fungi including Candida albicans, the major systemic fungal pathogen of humans. Here, we describe a proteomic comparison of the GCN responses in S. cerevisiae and C. albicans. We have used high-resolution two-dimensional (2-D) gel electrophoresis and peptide mass fingerprinting to develop a 2-D protein map of C. albicans. A total of 391 protein spots, representing 316 open reading frames, were identified. Fifty-five C. albicans and 65 S. cerevisiae proteins were identified that responded reproducibly to 3-aminotriazole (3AT) in a Gcn4p-dependent fashion. The changes in the S. cerevisiae proteome correlated with the response in the S. cerevisiae transcript profile to 3AT treatment (rank correlation coefficient = 0.59; Natarajan et al, Molec. Cell. Biol. 2001, 21, 4347-4368). Significant aspects of the GCN response were conserved in C. albicans and S. cerevisiae. In both fungi, amino acid biosynthetic enzymes on multiple metabolic pathways were induced by 3AT in a Gcn4p-dependent fashion. Carbon metabolism functions were also induced. However, subtle differences were observed between these fungi. For example, purine biosynthetic enzymes were induced in S. cerevisiae, but were not significantly induced in C. albicans. These differences presumably reflect the contrasting niches of these relatively benign and pathogenic yeasts, respectively.

Original languageEnglish
Pages (from-to)2425-2436
Number of pages11
JournalProteomics
Volume4
Issue number8
DOIs
Publication statusPublished - 2004

Keywords

  • amino acid starvation
  • Candida albicans
  • GCN4
  • Saccharomyces cerevisiae
  • PROTEIN-KINASE GCN2
  • GENE-EXPRESSION
  • MESSENGER-RNA
  • TRANSLATIONAL CONTROL
  • NEUROSPORA-CRASSA
  • YEAST
  • BIOSYNTHESIS
  • MORPHOGENESIS
  • VIRULENCE
  • SEQUENCE

Cite this

Yin, Z., Stead, D. A., Selway, L., Walker, J., Riba-Garcia, I., McInerney, T., ... Brown, A. J. P. (2004). Proteomic response to amino acid starvation in Candida albicans and Saccharomyces cerevisiae. Proteomics, 4(8), 2425-2436. https://doi.org/10.1002/pmic.200300760

Proteomic response to amino acid starvation in Candida albicans and Saccharomyces cerevisiae. / Yin, Zhikang; Stead, David Andrew; Selway, Laura; Walker, Janet; Riba-Garcia, I.; McInerney, T.; Gaskell, S.; Oliver, S. G.; Cash, Phillip; Brown, Alistair James Petersen.

In: Proteomics, Vol. 4, No. 8, 2004, p. 2425-2436.

Research output: Contribution to journalArticle

Yin, Z, Stead, DA, Selway, L, Walker, J, Riba-Garcia, I, McInerney, T, Gaskell, S, Oliver, SG, Cash, P & Brown, AJP 2004, 'Proteomic response to amino acid starvation in Candida albicans and Saccharomyces cerevisiae', Proteomics, vol. 4, no. 8, pp. 2425-2436. https://doi.org/10.1002/pmic.200300760
Yin, Zhikang ; Stead, David Andrew ; Selway, Laura ; Walker, Janet ; Riba-Garcia, I. ; McInerney, T. ; Gaskell, S. ; Oliver, S. G. ; Cash, Phillip ; Brown, Alistair James Petersen. / Proteomic response to amino acid starvation in Candida albicans and Saccharomyces cerevisiae. In: Proteomics. 2004 ; Vol. 4, No. 8. pp. 2425-2436.
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AU - Yin, Zhikang

AU - Stead, David Andrew

AU - Selway, Laura

AU - Walker, Janet

AU - Riba-Garcia, I.

AU - McInerney, T.

AU - Gaskell, S.

AU - Oliver, S. G.

AU - Cash, Phillip

AU - Brown, Alistair James Petersen

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N2 - Saccharomyces cerevisiae activates general amino acid control (GCN) in response to amino acid starvation. Some aspects of this response are known to be conserved in other fungi including Candida albicans, the major systemic fungal pathogen of humans. Here, we describe a proteomic comparison of the GCN responses in S. cerevisiae and C. albicans. We have used high-resolution two-dimensional (2-D) gel electrophoresis and peptide mass fingerprinting to develop a 2-D protein map of C. albicans. A total of 391 protein spots, representing 316 open reading frames, were identified. Fifty-five C. albicans and 65 S. cerevisiae proteins were identified that responded reproducibly to 3-aminotriazole (3AT) in a Gcn4p-dependent fashion. The changes in the S. cerevisiae proteome correlated with the response in the S. cerevisiae transcript profile to 3AT treatment (rank correlation coefficient = 0.59; Natarajan et al, Molec. Cell. Biol. 2001, 21, 4347-4368). Significant aspects of the GCN response were conserved in C. albicans and S. cerevisiae. In both fungi, amino acid biosynthetic enzymes on multiple metabolic pathways were induced by 3AT in a Gcn4p-dependent fashion. Carbon metabolism functions were also induced. However, subtle differences were observed between these fungi. For example, purine biosynthetic enzymes were induced in S. cerevisiae, but were not significantly induced in C. albicans. These differences presumably reflect the contrasting niches of these relatively benign and pathogenic yeasts, respectively.

AB - Saccharomyces cerevisiae activates general amino acid control (GCN) in response to amino acid starvation. Some aspects of this response are known to be conserved in other fungi including Candida albicans, the major systemic fungal pathogen of humans. Here, we describe a proteomic comparison of the GCN responses in S. cerevisiae and C. albicans. We have used high-resolution two-dimensional (2-D) gel electrophoresis and peptide mass fingerprinting to develop a 2-D protein map of C. albicans. A total of 391 protein spots, representing 316 open reading frames, were identified. Fifty-five C. albicans and 65 S. cerevisiae proteins were identified that responded reproducibly to 3-aminotriazole (3AT) in a Gcn4p-dependent fashion. The changes in the S. cerevisiae proteome correlated with the response in the S. cerevisiae transcript profile to 3AT treatment (rank correlation coefficient = 0.59; Natarajan et al, Molec. Cell. Biol. 2001, 21, 4347-4368). Significant aspects of the GCN response were conserved in C. albicans and S. cerevisiae. In both fungi, amino acid biosynthetic enzymes on multiple metabolic pathways were induced by 3AT in a Gcn4p-dependent fashion. Carbon metabolism functions were also induced. However, subtle differences were observed between these fungi. For example, purine biosynthetic enzymes were induced in S. cerevisiae, but were not significantly induced in C. albicans. These differences presumably reflect the contrasting niches of these relatively benign and pathogenic yeasts, respectively.

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KW - PROTEIN-KINASE GCN2

KW - GENE-EXPRESSION

KW - MESSENGER-RNA

KW - TRANSLATIONAL CONTROL

KW - NEUROSPORA-CRASSA

KW - YEAST

KW - BIOSYNTHESIS

KW - MORPHOGENESIS

KW - VIRULENCE

KW - SEQUENCE

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DO - 10.1002/pmic.200300760

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JO - Proteomics

JF - Proteomics

SN - 1615-9853

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ER -