Proteomics: a new approach to the study of disease

G Chambers, L Lawrie, P Cash, G I Murray

Research output: Contribution to journalLiterature review

177 Citations (Scopus)

Abstract

The global analysis of cellular proteins has recently been termed proteomics and is a key area of research that is developing in the post-genome era. Proteomics uses a combination of sophisticated techniques including two-dimensional (2D) gel electrophoresis, image analysis, mass spectrometry, amino acid sequencing, and bio-informatics to resolve comprehensively, to quantify, and to characterize proteins. The application of proteomics provides major opportunities to elucidate disease mechanisms and to identify new diagnostic markers and therapeutic targets. This review aims to explain briefly the background to proteomics and then to outline proteomic techniques. Applications to the study of human disease conditions ranging from cancer to infectious diseases are reviewed. Finally, possible future advances are briefly considered, especially those which may lead to faster sample throughput and increased sensitivity for the detection of individual proteins. Copyright (C) 2000 John Wiley & Sons, Ltd.

Original languageEnglish
Pages (from-to)280-288
Number of pages9
JournalThe Journal of pathology
Volume192
Publication statusPublished - 2000

Keywords

  • proteomics
  • gel electrophoresis
  • tumour
  • Alzheimer's disease
  • cardiomyopathy
  • 2-DIMENSIONAL GEL-ELECTROPHORESIS
  • PROTEIN DATABASES
  • MASS-SPECTROMETRY
  • HELICOBACTER-PYLORI
  • SEQUENCE DATABASES
  • POLYACRYLAMIDE-GEL
  • GENE-EXPRESSION
  • BLADDER-CANCER
  • BRAIN PROTEINS
  • SYPRO ORANGE

Cite this

Chambers, G., Lawrie, L., Cash, P., & Murray, G. I. (2000). Proteomics: a new approach to the study of disease. The Journal of pathology, 192, 280-288.