TY - JOUR
T1 - Proteomics
T2 - A Tool to Study Platelet Function
AU - Shevchuk, Olga
AU - Begonja, Antonija Jurak
AU - Gambaryan, Stepan
AU - Totzeck, Matthias
AU - Rassaf, Tienush
AU - Huber, Tobias B
AU - Greinacher, Andreas
AU - Renne, Thomas
AU - Sickmann, Albert
N1 - Acknowledgments: Authors acknowledge Laxmikanth Kollipara for the critical review and Julia Lill for support with figures preparation. The Figure 2 was created in Biorender.
PY - 2021/4/30
Y1 - 2021/4/30
N2 - Platelets are components of the blood that are highly reactive, and they quickly respond to multiple physiological and pathophysiological processes. In the last decade, it became clear that platelets are the key components of circulation, linking hemostasis, innate, and acquired immunity. Protein composition, localization, and activity are crucial for platelet function and regulation. The current state of mass spectrometry-based proteomics has tremendous potential to identify and quantify thousands of proteins from a minimal amount of material, unravel multiple post-translational modifications, and monitor platelet activity during drug treatments. This review focuses on the role of proteomics in understanding the molecular basics of the classical and newly emerging functions of platelets. including the recently described role of platelets in immunology and the development of COVID-19.The state-of-the-art proteomic technologies and their application in studying platelet biogenesis, signaling, and storage are described, and the potential of newly appeared trapped ion mobility spectrometry (TIMS) is highlighted. Additionally, implementing proteomic methods in platelet transfusion medicine, and as a diagnostic and prognostic tool, is discussed.
AB - Platelets are components of the blood that are highly reactive, and they quickly respond to multiple physiological and pathophysiological processes. In the last decade, it became clear that platelets are the key components of circulation, linking hemostasis, innate, and acquired immunity. Protein composition, localization, and activity are crucial for platelet function and regulation. The current state of mass spectrometry-based proteomics has tremendous potential to identify and quantify thousands of proteins from a minimal amount of material, unravel multiple post-translational modifications, and monitor platelet activity during drug treatments. This review focuses on the role of proteomics in understanding the molecular basics of the classical and newly emerging functions of platelets. including the recently described role of platelets in immunology and the development of COVID-19.The state-of-the-art proteomic technologies and their application in studying platelet biogenesis, signaling, and storage are described, and the potential of newly appeared trapped ion mobility spectrometry (TIMS) is highlighted. Additionally, implementing proteomic methods in platelet transfusion medicine, and as a diagnostic and prognostic tool, is discussed.
KW - LC-MS/MS
KW - PTMs
KW - mass spectrometry
KW - phosphoproteomics
KW - platelet transfusion proteomics
KW - platelets
KW - precision medicine
KW - signaling
KW - targeted proteomics
U2 - 10.3390/ijms22094776
DO - 10.3390/ijms22094776
M3 - Review article
C2 - 33946341
VL - 22
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 9
M1 - 4776
ER -