Proteomics in medical microbiology

P Cash

Research output: Contribution to journalLiterature review

62 Citations (Scopus)

Abstract

The techniques of proteomics (high resolution two-dimensional electrophoresis and protein characterisation) are widely used for microbiological research to analyse global protein synthesis as an indicator of gene expression. The rapid progress in microbial proteomics has been achieved through the wide availability of whole genome sequences for a number of bacterial groups. Beyond providing a basic understanding of microbial gene expression, proteomics has also played a role in medical areas of microbiology. Progress has been made in the use of the techniques for investigating the epidemiology and taxonomy of human microbial pathogens, the identification of novel pathogenic mechanisms and the analysis of drug resistance. In each of these areas, proteomics has provided new insights that complement genomic-based investigations. This review describes the current progress in these research fields and highlights some of the technical challenges existing for the application of proteomics in medical microbiology. The latter concern the analysis of genetically heterogeneous bacterial populations and the integration of the proteomic and genomic data for these bacteria. The characterisation of the proteomes of bacterial pathogens growing in their natural hosts remains a future challenge.

Original languageEnglish
Pages (from-to)1187-1201
Number of pages15
JournalElectrophoresis
Volume21
Issue number6
DOIs
Publication statusPublished - Apr 2000

Keywords

  • bacteria
  • pathogenesis
  • proteins
  • proteomics
  • medical microbiology
  • review
  • 2-dimensional gel-electrophoresis
  • tuberculosis culture filtrate
  • influenzae type-B
  • respiratory synctial virus
  • coli Swiss-2Dpage database
  • complete genome sequence
  • mycobacterium-bovis BCG
  • human-antibody response
  • amino-acid-composition
  • human epithelial-cells

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