Purification and functional properties of the membrane fissioning protein CtBP3/BARS

Carmen Valente; Stefania Spanò; Alberto Luini; Daniela Corda

doi:10.1016/S0076-6879(05)04027-9

Purification and functional properties of the membrane fissioning protein CtBP3/BARS

Carmen Valente, Stefania Spanò, Alberto Luini, Daniela Corda

Medical Sciences

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The fissioning protein CtBP3/BARS is a member of the CtBP transcription corepressor family of proteins. The characterization of this fissioning activity of CtBP3/BARS in both isolated Golgi membranes and in intact cells has indicated that the CtBP family includes multifunctional proteins that can act both in the nucleus and in the cytoplasm. The fissiogenic activity of CtBP3/BARS has a role in the fragmentation of the Golgi complex during mitosis and during intracellular membrane transport. This was demonstrated using a number of approaches and reagents, which are discussed in the following text, and which include recombinant proteins and mutants, antibodies, protein overexpression, RNA interference, antisense oligonucleotides, cell permeabilization, and electron miscroscopy, together with biochemical assays such as that for ADP-ribosylation.

Original language	English
Pages (from-to)	296-316
Number of pages	21
Journal	Methods in Enzymology
Volume	404
DOIs	https://doi.org/10.1016/S0076-6879(05)04027-9
Publication status	Published - 2005

Keywords

Acyl Coenzyme A
Acyltransferases
Animals
Carrier Proteins
Golgi Apparatus
Humans
Mutation
NAD
RNA, Small Interfering
Rats
Transcription Factors
Transfection

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10.1016/S0076-6879(05)04027-9

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title = "Purification and functional properties of the membrane fissioning protein CtBP3/BARS",

abstract = "The fissioning protein CtBP3/BARS is a member of the CtBP transcription corepressor family of proteins. The characterization of this fissioning activity of CtBP3/BARS in both isolated Golgi membranes and in intact cells has indicated that the CtBP family includes multifunctional proteins that can act both in the nucleus and in the cytoplasm. The fissiogenic activity of CtBP3/BARS has a role in the fragmentation of the Golgi complex during mitosis and during intracellular membrane transport. This was demonstrated using a number of approaches and reagents, which are discussed in the following text, and which include recombinant proteins and mutants, antibodies, protein overexpression, RNA interference, antisense oligonucleotides, cell permeabilization, and electron miscroscopy, together with biochemical assays such as that for ADP-ribosylation.",

keywords = "Acyl Coenzyme A, Acyltransferases, Animals, Carrier Proteins, Golgi Apparatus, Humans, Mutation, NAD, RNA, Small Interfering, Rats, Transcription Factors, Transfection",

author = "Carmen Valente and Stefania Span{\`o} and Alberto Luini and Daniela Corda",

year = "2005",

doi = "10.1016/S0076-6879(05)04027-9",

language = "English",

volume = "404",

pages = "296--316",

journal = "Methods in Enzymology",

issn = "0076-6879",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Purification and functional properties of the membrane fissioning protein CtBP3/BARS

AU - Valente, Carmen

AU - Spanò, Stefania

AU - Luini, Alberto

AU - Corda, Daniela

PY - 2005

Y1 - 2005

N2 - The fissioning protein CtBP3/BARS is a member of the CtBP transcription corepressor family of proteins. The characterization of this fissioning activity of CtBP3/BARS in both isolated Golgi membranes and in intact cells has indicated that the CtBP family includes multifunctional proteins that can act both in the nucleus and in the cytoplasm. The fissiogenic activity of CtBP3/BARS has a role in the fragmentation of the Golgi complex during mitosis and during intracellular membrane transport. This was demonstrated using a number of approaches and reagents, which are discussed in the following text, and which include recombinant proteins and mutants, antibodies, protein overexpression, RNA interference, antisense oligonucleotides, cell permeabilization, and electron miscroscopy, together with biochemical assays such as that for ADP-ribosylation.

AB - The fissioning protein CtBP3/BARS is a member of the CtBP transcription corepressor family of proteins. The characterization of this fissioning activity of CtBP3/BARS in both isolated Golgi membranes and in intact cells has indicated that the CtBP family includes multifunctional proteins that can act both in the nucleus and in the cytoplasm. The fissiogenic activity of CtBP3/BARS has a role in the fragmentation of the Golgi complex during mitosis and during intracellular membrane transport. This was demonstrated using a number of approaches and reagents, which are discussed in the following text, and which include recombinant proteins and mutants, antibodies, protein overexpression, RNA interference, antisense oligonucleotides, cell permeabilization, and electron miscroscopy, together with biochemical assays such as that for ADP-ribosylation.

KW - Acyl Coenzyme A

KW - Acyltransferases

KW - Animals

KW - Carrier Proteins

KW - Golgi Apparatus

KW - Humans

KW - Mutation

KW - NAD

KW - RNA, Small Interfering

KW - Rats

KW - Transcription Factors

KW - Transfection

U2 - 10.1016/S0076-6879(05)04027-9

DO - 10.1016/S0076-6879(05)04027-9

M3 - Article

C2 - 16413278

SN - 0076-6879

VL - 404

SP - 296

EP - 316

JO - Methods in Enzymology

JF - Methods in Enzymology

ER -

Purification and functional properties of the membrane fissioning protein CtBP3/BARS

Abstract

Keywords

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