PURIFICATION AND INITIAL CHARACTERIZATION OF AHRC - THE REGULATOR OF ARGININE METABOLISM GENES IN BACILLUS-SUBTILIS

L G CZAPLEWSKI, A K NORTH, Margaret Caroline MacHin Smith, S BAUMBERG, P G STOCKLEY

Research output: Contribution to journalArticle

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Abstract

The arginine-dependent repressor-activator from Bacillus subtilis, AhrC, has been overexpressed in Escherichia coli and purified to homogeneity. AhrC, expressed in E. coli, is able to repress a Bacillus promoter (argC(p)), which lies upstream of the argC gene. The purified protein is a hexamer with a subunit molecular mass of 16.7 kDa. Its ability to recognize DNA has been examined in vitro using argC(p) in both DNase I and hydroxyl radical protection assays. AhrC binds at two distinct sites within the argC(p) fragment. One site, argC(O1), with the highest affinity for protein, is located within the 5' promoter sequences, whilst the other, argC(O2), is within the coding region of argC. The data are consistent with the binding of a single hexamer of AhrC to argC(O1) via four of its subunits, possibly allowing the remaining two subunits to bind at argC(O2) in vivo forming a repression loop similar to those observed for the E. coli Lac repressor.

Original languageEnglish
Pages (from-to)267-275
Number of pages9
JournalMolecular Microbiology
Volume6
Issue number2
Publication statusPublished - Jan 1992

Keywords

  • ESCHERICHIA-COLI K-12
  • NUCLEOTIDE-SEQUENCE
  • SELF-ASSOCIATION
  • HIGH-RESOLUTION
  • REPRESSOR
  • DNA
  • OPERATOR
  • CLONING
  • PROTEIN
  • BIOSYNTHESIS

Cite this

CZAPLEWSKI, L. G., NORTH, A. K., Smith, M. C. M., BAUMBERG, S., & STOCKLEY, P. G. (1992). PURIFICATION AND INITIAL CHARACTERIZATION OF AHRC - THE REGULATOR OF ARGININE METABOLISM GENES IN BACILLUS-SUBTILIS. Molecular Microbiology, 6(2), 267-275.

PURIFICATION AND INITIAL CHARACTERIZATION OF AHRC - THE REGULATOR OF ARGININE METABOLISM GENES IN BACILLUS-SUBTILIS. / CZAPLEWSKI, L G ; NORTH, A K ; Smith, Margaret Caroline MacHin; BAUMBERG, S ; STOCKLEY, P G .

In: Molecular Microbiology, Vol. 6, No. 2, 01.1992, p. 267-275.

Research output: Contribution to journalArticle

CZAPLEWSKI, LG, NORTH, AK, Smith, MCM, BAUMBERG, S & STOCKLEY, PG 1992, 'PURIFICATION AND INITIAL CHARACTERIZATION OF AHRC - THE REGULATOR OF ARGININE METABOLISM GENES IN BACILLUS-SUBTILIS', Molecular Microbiology, vol. 6, no. 2, pp. 267-275.
CZAPLEWSKI, L G ; NORTH, A K ; Smith, Margaret Caroline MacHin ; BAUMBERG, S ; STOCKLEY, P G . / PURIFICATION AND INITIAL CHARACTERIZATION OF AHRC - THE REGULATOR OF ARGININE METABOLISM GENES IN BACILLUS-SUBTILIS. In: Molecular Microbiology. 1992 ; Vol. 6, No. 2. pp. 267-275.
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abstract = "The arginine-dependent repressor-activator from Bacillus subtilis, AhrC, has been overexpressed in Escherichia coli and purified to homogeneity. AhrC, expressed in E. coli, is able to repress a Bacillus promoter (argC(p)), which lies upstream of the argC gene. The purified protein is a hexamer with a subunit molecular mass of 16.7 kDa. Its ability to recognize DNA has been examined in vitro using argC(p) in both DNase I and hydroxyl radical protection assays. AhrC binds at two distinct sites within the argC(p) fragment. One site, argC(O1), with the highest affinity for protein, is located within the 5' promoter sequences, whilst the other, argC(O2), is within the coding region of argC. The data are consistent with the binding of a single hexamer of AhrC to argC(O1) via four of its subunits, possibly allowing the remaining two subunits to bind at argC(O2) in vivo forming a repression loop similar to those observed for the E. coli Lac repressor.",
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AB - The arginine-dependent repressor-activator from Bacillus subtilis, AhrC, has been overexpressed in Escherichia coli and purified to homogeneity. AhrC, expressed in E. coli, is able to repress a Bacillus promoter (argC(p)), which lies upstream of the argC gene. The purified protein is a hexamer with a subunit molecular mass of 16.7 kDa. Its ability to recognize DNA has been examined in vitro using argC(p) in both DNase I and hydroxyl radical protection assays. AhrC binds at two distinct sites within the argC(p) fragment. One site, argC(O1), with the highest affinity for protein, is located within the 5' promoter sequences, whilst the other, argC(O2), is within the coding region of argC. The data are consistent with the binding of a single hexamer of AhrC to argC(O1) via four of its subunits, possibly allowing the remaining two subunits to bind at argC(O2) in vivo forming a repression loop similar to those observed for the E. coli Lac repressor.

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