Purification and properties of glutamate-phenylpyruvate aminotransferase from the ruminal protozoan Entodinium caudatum

M R Amin, R Onodera, R I Khan, R J Wallace, C J Newbold

Research output: Contribution to journalArticle

Abstract

Entodinium species are important in catabolic protein metabolism by the mixed ruminal microbial population. This study was conducted to purify, and investigate properties of one of the enzymes involved in amino acid metabolism by Entodinium caudatum, glutamate-phenylpyruvate aminotransferase (GPA; EC 2.6.1.64). GPA was purified 74-fold from a cell-free extract by ammonium sulfate precipitation and column chromatography with phenyl-superose, DEAE-Toyopearl 650M, Sephacryl S-100 HR, and Sephadex G-100. The molecular mass of GPA was estimated by SDS-PAGE to be 65.0 kDa. The optimum pH was 6.0 and it was found to be reactive over a wide range of pH from 5.0 to 10.5. Maximum activity of GPA occurred at 45degreesC and the activity declined at temperatures over 55degreesC. GPA was stable below 60degreesC. Aminooxyacetate and phenylhydrazine were highly inhibitory, and SDS, EDTA, and some heavy metal ions also inhibited activity. The purification and characterisation of the enzyme will help to isolate the gene and ultimately to understand the role of GPA in both anabolic and catabolic amino acid metabolism by Entodinium caudatum.

Original languageEnglish
Pages (from-to)991-997
Number of pages7
JournalAustralian Journal of Agricultural Research
Volume55
Issue number9
DOIs
Publication statusPublished - Sep 2004

Keywords

  • rumen protozoa
  • transaminase
  • characterisation
  • mixture in-vitro
  • shep rumen
  • transaminase-activity
  • Escherichia coli
  • L-phenylalanine
  • acid
  • aspartate
  • bacteria
  • nitrogen
  • microorganisms

Cite this

Purification and properties of glutamate-phenylpyruvate aminotransferase from the ruminal protozoan Entodinium caudatum. / Amin, M R ; Onodera, R ; Khan, R I ; Wallace, R J; Newbold, C J.

In: Australian Journal of Agricultural Research, Vol. 55, No. 9, 09.2004, p. 991-997.

Research output: Contribution to journalArticle

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