Quantification of phytochelatins and their metal(loid) complexes: critical assessment of current analytical methodology

B. Alan Wood, Jörg Feldmann

Research output: Contribution to journalLiterature review

25 Citations (Scopus)

Abstract

Whilst there are a variety of methods available for the quantification of biothiols in sample extracts, each has their own inherent advantages and limitations. The ease with which thiols readily oxidise not only hinders their quantification but also alters the speciation profile. The challenge faced by the analyst is not only to preserve the speciation of the sample, but also to select a method which allows the retrieval of the desired information. Given that sulfur is not a chromophore and that it cannot easily be monitored by ICP-MS, a number of direct and indirect methods have been developed for this purpose. In order to assess these methods, they are compared in the context of the measurement of arsenic-phytochelatin complexes in plant extracts. The inherent instability of such complexes, along with the instabilities of reduced glutathione and phytochelatin species,necessitates a rapid and sensitive analytical protocol. Whilst being a specific example, the points raised and discussed in this review will also be applicable to the quantification of biothiols and thiol-metal(loid) species in a wide range of systems other than just the analysis of arsenic-phytochelatin species in plant extracts.

Original languageEnglish
Pages (from-to)3299-3309
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume402
Issue number10
Early online date10 Jan 2012
DOIs
Publication statusPublished - Apr 2012

Keywords

  • bioanalytical methods
  • mass spectrometry/ICP-MS
  • speciation
  • biological samples
  • sulfur

Cite this

Quantification of phytochelatins and their metal(loid) complexes : critical assessment of current analytical methodology. / Wood, B. Alan; Feldmann, Jörg.

In: Analytical and Bioanalytical Chemistry, Vol. 402, No. 10, 04.2012, p. 3299-3309.

Research output: Contribution to journalLiterature review

@article{d104ea5dc974436eb44ec6fa5c95a48b,
title = "Quantification of phytochelatins and their metal(loid) complexes: critical assessment of current analytical methodology",
abstract = "Whilst there are a variety of methods available for the quantification of biothiols in sample extracts, each has their own inherent advantages and limitations. The ease with which thiols readily oxidise not only hinders their quantification but also alters the speciation profile. The challenge faced by the analyst is not only to preserve the speciation of the sample, but also to select a method which allows the retrieval of the desired information. Given that sulfur is not a chromophore and that it cannot easily be monitored by ICP-MS, a number of direct and indirect methods have been developed for this purpose. In order to assess these methods, they are compared in the context of the measurement of arsenic-phytochelatin complexes in plant extracts. The inherent instability of such complexes, along with the instabilities of reduced glutathione and phytochelatin species,necessitates a rapid and sensitive analytical protocol. Whilst being a specific example, the points raised and discussed in this review will also be applicable to the quantification of biothiols and thiol-metal(loid) species in a wide range of systems other than just the analysis of arsenic-phytochelatin species in plant extracts.",
keywords = "bioanalytical methods, mass spectrometry/ICP-MS, speciation, biological samples, sulfur",
author = "Wood, {B. Alan} and J{\"o}rg Feldmann",
year = "2012",
month = "4",
doi = "10.1007/s00216-011-5649-0",
language = "English",
volume = "402",
pages = "3299--3309",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "Springer Verlag",
number = "10",

}

TY - JOUR

T1 - Quantification of phytochelatins and their metal(loid) complexes

T2 - critical assessment of current analytical methodology

AU - Wood, B. Alan

AU - Feldmann, Jörg

PY - 2012/4

Y1 - 2012/4

N2 - Whilst there are a variety of methods available for the quantification of biothiols in sample extracts, each has their own inherent advantages and limitations. The ease with which thiols readily oxidise not only hinders their quantification but also alters the speciation profile. The challenge faced by the analyst is not only to preserve the speciation of the sample, but also to select a method which allows the retrieval of the desired information. Given that sulfur is not a chromophore and that it cannot easily be monitored by ICP-MS, a number of direct and indirect methods have been developed for this purpose. In order to assess these methods, they are compared in the context of the measurement of arsenic-phytochelatin complexes in plant extracts. The inherent instability of such complexes, along with the instabilities of reduced glutathione and phytochelatin species,necessitates a rapid and sensitive analytical protocol. Whilst being a specific example, the points raised and discussed in this review will also be applicable to the quantification of biothiols and thiol-metal(loid) species in a wide range of systems other than just the analysis of arsenic-phytochelatin species in plant extracts.

AB - Whilst there are a variety of methods available for the quantification of biothiols in sample extracts, each has their own inherent advantages and limitations. The ease with which thiols readily oxidise not only hinders their quantification but also alters the speciation profile. The challenge faced by the analyst is not only to preserve the speciation of the sample, but also to select a method which allows the retrieval of the desired information. Given that sulfur is not a chromophore and that it cannot easily be monitored by ICP-MS, a number of direct and indirect methods have been developed for this purpose. In order to assess these methods, they are compared in the context of the measurement of arsenic-phytochelatin complexes in plant extracts. The inherent instability of such complexes, along with the instabilities of reduced glutathione and phytochelatin species,necessitates a rapid and sensitive analytical protocol. Whilst being a specific example, the points raised and discussed in this review will also be applicable to the quantification of biothiols and thiol-metal(loid) species in a wide range of systems other than just the analysis of arsenic-phytochelatin species in plant extracts.

KW - bioanalytical methods

KW - mass spectrometry/ICP-MS

KW - speciation

KW - biological samples

KW - sulfur

U2 - 10.1007/s00216-011-5649-0

DO - 10.1007/s00216-011-5649-0

M3 - Literature review

VL - 402

SP - 3299

EP - 3309

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 10

ER -