Quantitative analysis of tumor in bronchial biopsy specimens

Caroline Coghlin, Louise J Smith, Salmah Bakar, Keith N Stewart, Graham S Devereux, Marianne C Nicolson, Keith M Kerr

Research output: Contribution to journalArticle

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Abstract

BACKGROUND: Diagnostic bronchial biopsy samples from lung cancer patients may be used for molecular biologic analyses to help select therapy and provide prognostic information. Some have suggested that direct molecular analysis of bronchial biopsy fragments may be feasible, bypassing histologic examination. We analyzed a series of 100 bronchial biopsy specimens in lung cancer patients to assess the frequency and quantity of tumor present in biopsy samples. METHODS: The proportion of tumor in bronchial biopsy specimens was assessed by measuring the tumor area in histologic sections using computer-aided morphometry. RESULTS: In only 48% of cases did all the biopsy fragments contain some tumor. The median number of fragments obtained at bronchoscopy was 4; median number actually containing tumor was 3. The mean total surface area of tumor (as a percentage of the total sample area) in biopsy fragments was, for all cases, 33.4%; median area 28%. Biopsies with small cell carcinoma had more tumor (mean area 46.5%, median 49%; p = 0.0006) than all other non-small cell carcinoma cases. CONCLUSION: Malignant bronchial biopsy samples frequently contain limited amounts of primary carcinoma. Often, one or more of the biopsy fragments will not contain tumor. This has important implications for the storage and use of bronchial biopsy samples for genetic analysis.
Original languageEnglish
Pages (from-to)448-552
Number of pages5
JournalJournal of Thoracic Oncology
Volume5
Issue number4
DOIs
Publication statusPublished - Apr 2010

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Biopsy
Neoplasms
Lung Neoplasms
Carcinoma
Small Cell Carcinoma
Bronchoscopy

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Coghlin, C., Smith, L. J., Bakar, S., Stewart, K. N., Devereux, G. S., Nicolson, M. C., & Kerr, K. M. (2010). Quantitative analysis of tumor in bronchial biopsy specimens. Journal of Thoracic Oncology, 5(4), 448-552. https://doi.org/10.1097/JTO.0b013e3181ca12c4

Quantitative analysis of tumor in bronchial biopsy specimens. / Coghlin, Caroline; Smith, Louise J; Bakar, Salmah; Stewart, Keith N; Devereux, Graham S; Nicolson, Marianne C; Kerr, Keith M.

In: Journal of Thoracic Oncology, Vol. 5, No. 4, 04.2010, p. 448-552.

Research output: Contribution to journalArticle

Coghlin, C, Smith, LJ, Bakar, S, Stewart, KN, Devereux, GS, Nicolson, MC & Kerr, KM 2010, 'Quantitative analysis of tumor in bronchial biopsy specimens', Journal of Thoracic Oncology, vol. 5, no. 4, pp. 448-552. https://doi.org/10.1097/JTO.0b013e3181ca12c4
Coghlin, Caroline ; Smith, Louise J ; Bakar, Salmah ; Stewart, Keith N ; Devereux, Graham S ; Nicolson, Marianne C ; Kerr, Keith M. / Quantitative analysis of tumor in bronchial biopsy specimens. In: Journal of Thoracic Oncology. 2010 ; Vol. 5, No. 4. pp. 448-552.
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abstract = "BACKGROUND: Diagnostic bronchial biopsy samples from lung cancer patients may be used for molecular biologic analyses to help select therapy and provide prognostic information. Some have suggested that direct molecular analysis of bronchial biopsy fragments may be feasible, bypassing histologic examination. We analyzed a series of 100 bronchial biopsy specimens in lung cancer patients to assess the frequency and quantity of tumor present in biopsy samples. METHODS: The proportion of tumor in bronchial biopsy specimens was assessed by measuring the tumor area in histologic sections using computer-aided morphometry. RESULTS: In only 48{\%} of cases did all the biopsy fragments contain some tumor. The median number of fragments obtained at bronchoscopy was 4; median number actually containing tumor was 3. The mean total surface area of tumor (as a percentage of the total sample area) in biopsy fragments was, for all cases, 33.4{\%}; median area 28{\%}. Biopsies with small cell carcinoma had more tumor (mean area 46.5{\%}, median 49{\%}; p = 0.0006) than all other non-small cell carcinoma cases. CONCLUSION: Malignant bronchial biopsy samples frequently contain limited amounts of primary carcinoma. Often, one or more of the biopsy fragments will not contain tumor. This has important implications for the storage and use of bronchial biopsy samples for genetic analysis.",
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AB - BACKGROUND: Diagnostic bronchial biopsy samples from lung cancer patients may be used for molecular biologic analyses to help select therapy and provide prognostic information. Some have suggested that direct molecular analysis of bronchial biopsy fragments may be feasible, bypassing histologic examination. We analyzed a series of 100 bronchial biopsy specimens in lung cancer patients to assess the frequency and quantity of tumor present in biopsy samples. METHODS: The proportion of tumor in bronchial biopsy specimens was assessed by measuring the tumor area in histologic sections using computer-aided morphometry. RESULTS: In only 48% of cases did all the biopsy fragments contain some tumor. The median number of fragments obtained at bronchoscopy was 4; median number actually containing tumor was 3. The mean total surface area of tumor (as a percentage of the total sample area) in biopsy fragments was, for all cases, 33.4%; median area 28%. Biopsies with small cell carcinoma had more tumor (mean area 46.5%, median 49%; p = 0.0006) than all other non-small cell carcinoma cases. CONCLUSION: Malignant bronchial biopsy samples frequently contain limited amounts of primary carcinoma. Often, one or more of the biopsy fragments will not contain tumor. This has important implications for the storage and use of bronchial biopsy samples for genetic analysis.

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