Quantitative partition of threonine oxidation in pigs: effect of dietary threonine

O Ballevre, A Cadenhead, A G Calder, W Rees, G Lobley, M F Fuller, P J Garlick

Research output: Contribution to journalArticle

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Abstract

Kinetic aspects of threonine (Thr) metabolism were examined in eight pigs fed hourly with a diet containing either 0.68% (LT group) or 0.81% (HT group) of Thr (wt/wt), corresponding to 10 and 30% Thr excess, respectively, compared with an "ideal" diet. Primary production (PR) and disposal (DR) rates were obtained for Thr, glycine (Gly), and 2-keto-butyrate (KB) after a 12-h continuous infusion of L-[U-14C]-Thr together with [1-13C]Gly and a 6-h continuous infusion of [1-14C]KB. Transfer of Thr into secondary pools was also monitored, and from these the rates of Thr oxidation through the catabolic pathways of L-Thr 3-dehydrogenase (DR(Thr-Gly)) and threonine dehydratase (DR(Thr-KB)) were estimated. For the LT group the results were (mumol.kg-1.h-1) PR(Thr) 314 +/- 3, PR(Gly) 551 +/- 24, PR(KB) 41 +/- 3, DR(Thr-Gly) 22 +/- 2, and DR(Thr-KB) 7 +/- 1. For the HT group they were PR(Thr) 301 +/- 23, PR(Gly) 598 +/- 55, PR(KB) 39 +/- 4, DR(Thr-Gly) 32 +/- 2, and DR(Thr-KB) 8 +/- 1. The increase in Thr intake (14 mumol.kg-1.h-1, P less than 0.01) induced a commensurate increase in the sum of DR(Thr-Gly) and DR(Thr-KB) (14 mumol.kg-1.h-1, P less than 0.001) when liver was used as the precursor pool. This was mainly due to the increased DR(Thr-Gly) (13 mumol.kg-1.h-1, P less than 0.01); the change in DR(Thr-KB) was not statistically significant. By comparison of intracellular-to-plasma ratios of specific activities (or enrichments) for different tissues with each type of infusion, liver was shown to be the major site of production of Gly and KB from Thr. These data suggest that in fed growing pigs a 30% excess of Thr in the diet does not alter the partition of Thr oxidation, since 80% of Thr oxidation occurs through the L-Thr 3-dehydrogenase pathway for both LT and HT groups.
Original languageEnglish
Pages (from-to)E483-E491
Number of pages9
JournalAmerican Journal of Physiology
Volume259
Issue number4 Pt 1
Publication statusPublished - 1 Oct 1990

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Threonine
Swine
Butyrates
Glycine
L-threonine 3-dehydrogenase
Diet
Threonine Dehydratase

Keywords

  • amino acids
  • animals
  • body weight
  • carbon radioisotopes
  • diet
  • models, biological
  • oxidation-reduction
  • protein biosynthesis
  • proteins
  • radioisotope dilution technique
  • swine
  • threonine

Cite this

Ballevre, O., Cadenhead, A., Calder, A. G., Rees, W., Lobley, G., Fuller, M. F., & Garlick, P. J. (1990). Quantitative partition of threonine oxidation in pigs: effect of dietary threonine. American Journal of Physiology, 259(4 Pt 1), E483-E491.

Quantitative partition of threonine oxidation in pigs : effect of dietary threonine. / Ballevre, O; Cadenhead, A; Calder, A G; Rees, W; Lobley, G; Fuller, M F; Garlick, P J.

In: American Journal of Physiology, Vol. 259, No. 4 Pt 1, 01.10.1990, p. E483-E491.

Research output: Contribution to journalArticle

Ballevre, O, Cadenhead, A, Calder, AG, Rees, W, Lobley, G, Fuller, MF & Garlick, PJ 1990, 'Quantitative partition of threonine oxidation in pigs: effect of dietary threonine', American Journal of Physiology, vol. 259, no. 4 Pt 1, pp. E483-E491.
Ballevre O, Cadenhead A, Calder AG, Rees W, Lobley G, Fuller MF et al. Quantitative partition of threonine oxidation in pigs: effect of dietary threonine. American Journal of Physiology. 1990 Oct 1;259(4 Pt 1):E483-E491.
Ballevre, O ; Cadenhead, A ; Calder, A G ; Rees, W ; Lobley, G ; Fuller, M F ; Garlick, P J. / Quantitative partition of threonine oxidation in pigs : effect of dietary threonine. In: American Journal of Physiology. 1990 ; Vol. 259, No. 4 Pt 1. pp. E483-E491.
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abstract = "Kinetic aspects of threonine (Thr) metabolism were examined in eight pigs fed hourly with a diet containing either 0.68{\%} (LT group) or 0.81{\%} (HT group) of Thr (wt/wt), corresponding to 10 and 30{\%} Thr excess, respectively, compared with an {"}ideal{"} diet. Primary production (PR) and disposal (DR) rates were obtained for Thr, glycine (Gly), and 2-keto-butyrate (KB) after a 12-h continuous infusion of L-[U-14C]-Thr together with [1-13C]Gly and a 6-h continuous infusion of [1-14C]KB. Transfer of Thr into secondary pools was also monitored, and from these the rates of Thr oxidation through the catabolic pathways of L-Thr 3-dehydrogenase (DR(Thr-Gly)) and threonine dehydratase (DR(Thr-KB)) were estimated. For the LT group the results were (mumol.kg-1.h-1) PR(Thr) 314 +/- 3, PR(Gly) 551 +/- 24, PR(KB) 41 +/- 3, DR(Thr-Gly) 22 +/- 2, and DR(Thr-KB) 7 +/- 1. For the HT group they were PR(Thr) 301 +/- 23, PR(Gly) 598 +/- 55, PR(KB) 39 +/- 4, DR(Thr-Gly) 32 +/- 2, and DR(Thr-KB) 8 +/- 1. The increase in Thr intake (14 mumol.kg-1.h-1, P less than 0.01) induced a commensurate increase in the sum of DR(Thr-Gly) and DR(Thr-KB) (14 mumol.kg-1.h-1, P less than 0.001) when liver was used as the precursor pool. This was mainly due to the increased DR(Thr-Gly) (13 mumol.kg-1.h-1, P less than 0.01); the change in DR(Thr-KB) was not statistically significant. By comparison of intracellular-to-plasma ratios of specific activities (or enrichments) for different tissues with each type of infusion, liver was shown to be the major site of production of Gly and KB from Thr. These data suggest that in fed growing pigs a 30{\%} excess of Thr in the diet does not alter the partition of Thr oxidation, since 80{\%} of Thr oxidation occurs through the L-Thr 3-dehydrogenase pathway for both LT and HT groups.",
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T1 - Quantitative partition of threonine oxidation in pigs

T2 - effect of dietary threonine

AU - Ballevre, O

AU - Cadenhead, A

AU - Calder, A G

AU - Rees, W

AU - Lobley, G

AU - Fuller, M F

AU - Garlick, P J

PY - 1990/10/1

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N2 - Kinetic aspects of threonine (Thr) metabolism were examined in eight pigs fed hourly with a diet containing either 0.68% (LT group) or 0.81% (HT group) of Thr (wt/wt), corresponding to 10 and 30% Thr excess, respectively, compared with an "ideal" diet. Primary production (PR) and disposal (DR) rates were obtained for Thr, glycine (Gly), and 2-keto-butyrate (KB) after a 12-h continuous infusion of L-[U-14C]-Thr together with [1-13C]Gly and a 6-h continuous infusion of [1-14C]KB. Transfer of Thr into secondary pools was also monitored, and from these the rates of Thr oxidation through the catabolic pathways of L-Thr 3-dehydrogenase (DR(Thr-Gly)) and threonine dehydratase (DR(Thr-KB)) were estimated. For the LT group the results were (mumol.kg-1.h-1) PR(Thr) 314 +/- 3, PR(Gly) 551 +/- 24, PR(KB) 41 +/- 3, DR(Thr-Gly) 22 +/- 2, and DR(Thr-KB) 7 +/- 1. For the HT group they were PR(Thr) 301 +/- 23, PR(Gly) 598 +/- 55, PR(KB) 39 +/- 4, DR(Thr-Gly) 32 +/- 2, and DR(Thr-KB) 8 +/- 1. The increase in Thr intake (14 mumol.kg-1.h-1, P less than 0.01) induced a commensurate increase in the sum of DR(Thr-Gly) and DR(Thr-KB) (14 mumol.kg-1.h-1, P less than 0.001) when liver was used as the precursor pool. This was mainly due to the increased DR(Thr-Gly) (13 mumol.kg-1.h-1, P less than 0.01); the change in DR(Thr-KB) was not statistically significant. By comparison of intracellular-to-plasma ratios of specific activities (or enrichments) for different tissues with each type of infusion, liver was shown to be the major site of production of Gly and KB from Thr. These data suggest that in fed growing pigs a 30% excess of Thr in the diet does not alter the partition of Thr oxidation, since 80% of Thr oxidation occurs through the L-Thr 3-dehydrogenase pathway for both LT and HT groups.

AB - Kinetic aspects of threonine (Thr) metabolism were examined in eight pigs fed hourly with a diet containing either 0.68% (LT group) or 0.81% (HT group) of Thr (wt/wt), corresponding to 10 and 30% Thr excess, respectively, compared with an "ideal" diet. Primary production (PR) and disposal (DR) rates were obtained for Thr, glycine (Gly), and 2-keto-butyrate (KB) after a 12-h continuous infusion of L-[U-14C]-Thr together with [1-13C]Gly and a 6-h continuous infusion of [1-14C]KB. Transfer of Thr into secondary pools was also monitored, and from these the rates of Thr oxidation through the catabolic pathways of L-Thr 3-dehydrogenase (DR(Thr-Gly)) and threonine dehydratase (DR(Thr-KB)) were estimated. For the LT group the results were (mumol.kg-1.h-1) PR(Thr) 314 +/- 3, PR(Gly) 551 +/- 24, PR(KB) 41 +/- 3, DR(Thr-Gly) 22 +/- 2, and DR(Thr-KB) 7 +/- 1. For the HT group they were PR(Thr) 301 +/- 23, PR(Gly) 598 +/- 55, PR(KB) 39 +/- 4, DR(Thr-Gly) 32 +/- 2, and DR(Thr-KB) 8 +/- 1. The increase in Thr intake (14 mumol.kg-1.h-1, P less than 0.01) induced a commensurate increase in the sum of DR(Thr-Gly) and DR(Thr-KB) (14 mumol.kg-1.h-1, P less than 0.001) when liver was used as the precursor pool. This was mainly due to the increased DR(Thr-Gly) (13 mumol.kg-1.h-1, P less than 0.01); the change in DR(Thr-KB) was not statistically significant. By comparison of intracellular-to-plasma ratios of specific activities (or enrichments) for different tissues with each type of infusion, liver was shown to be the major site of production of Gly and KB from Thr. These data suggest that in fed growing pigs a 30% excess of Thr in the diet does not alter the partition of Thr oxidation, since 80% of Thr oxidation occurs through the L-Thr 3-dehydrogenase pathway for both LT and HT groups.

KW - amino acids

KW - animals

KW - body weight

KW - carbon radioisotopes

KW - diet

KW - models, biological

KW - oxidation-reduction

KW - protein biosynthesis

KW - proteins

KW - radioisotope dilution technique

KW - swine

KW - threonine

M3 - Article

C2 - 2121045

VL - 259

SP - E483-E491

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0002-9513

IS - 4 Pt 1

ER -