Quick and robust method for trace determination of MeHg in rice and rice products without derivatisation

C-C Brombach, M F Ezzeldin, B Chen, W T Corns, J Feldmann, E M Krupp

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Abstract

Recent studies of Methylmercury (MeHg) in rice have shown that rice grown on mercury contaminated soil contributes to the human MeHg intake similar to a fish diet. Trace levels of MeHg in biological samples are often determined via a complex multi-stage process following EPA method 1630. We developed a simple and cost effective method suited for food quality monitoring based on a simple sample preparation procedure and the subsequent analysis of the sample by online preconcentration - high performance liquid chromatography-cold vapor atomic fluorescence spectrometry (SPE-HPLC-CV-AFS). The reliability of this method for MeHg in rice and rice products in the low ppb range was investigated for 4 different rice product samples. At present, no CRM for MeHg in rice or rice products is available. Therefore we cross-validated our method against standard addition and species-specific isotope dilution gas chromatography inductively coupled plasma mass spectrometry (SSID-GC-ICP-MS), which showed no significant difference versus the external calibration with SPE-HPLC-CV-AFS. Potential species interconversion during sample preparation and measurement was ruled out by using a spike of isotopically enriched inorganic mercury. The preconcentration HPLC-CV-AFS developed in our work has proven to be a robust, fast, cost efficient, competitive and reliable method for MeHg speciation in rice and rice products with a limit of detection of 0.12 µg kg-1 and a reproducibility comparable to the SS-ID-GC-ICPMS method which is sufficient for the determination of MeHg concentration in the four market rice samples. The concentrations of MeHg ranged from 1.6 to 2.7 µg kg-1.
Original languageEnglish
Pages (from-to)8584-8589
Number of pages6
JournalAnalytical Methods
Volume7
Issue number20
Early online date7 Aug 2015
DOIs
Publication statusPublished - Oct 2015

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Mercury
Inductively coupled plasma mass spectrometry
High performance liquid chromatography
Inductively coupled plasma
Nutrition
Isotopes
Gas chromatography
Fish
Spectrometry
Dilution
Costs
Fluorescence
Vapors
Calibration
Soils
Monitoring

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Quick and robust method for trace determination of MeHg in rice and rice products without derivatisation. / Brombach, C-C; Ezzeldin, M F; Chen, B; Corns, W T; Feldmann, J; Krupp, E M.

In: Analytical Methods, Vol. 7, No. 20, 10.2015, p. 8584-8589.

Research output: Contribution to journalArticle

Brombach, C-C ; Ezzeldin, M F ; Chen, B ; Corns, W T ; Feldmann, J ; Krupp, E M. / Quick and robust method for trace determination of MeHg in rice and rice products without derivatisation. In: Analytical Methods. 2015 ; Vol. 7, No. 20. pp. 8584-8589.
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