Rainbow trout liver proteome: dietary manipulation and protein metabolism

Samuel Allen Moore Martin, Oddur T Vilhelmsson, Dominic Francis Joseph Houlihan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Changes in the expressed protein complement of rainbow trout livers have been studied using a proteomics approach. This report details the alterations of individual protein abundance in the livers of trout that have been subjected to dietary manipulations. These dietary changes have direct and indirect effects on protein turnover. Three experiments have been performed, 1) short term starvation, 2) addition of soy proteins to the diet, 3) use of plant protein as replacement of fish meal in the diet. For each experiment we have identified proteins that change in abundance and have used two programs, Mascot and MS-fit to identify proteins following trypsin digest fingerprinting.

Original languageEnglish
Title of host publicationProgress in Research on Energy and Protein Metabolism
Subtitle of host publicationEAAP Scientific
EditorsW.B. Souffrant, C.C. Metges
PublisherPurdue University Press
Pages57-60
Number of pages4
Volume109
ISBN (Print)9076998248, 9789076998244
Publication statusPublished - Apr 2004

Publication series

NameFAO agricultural development paper.
PublisherEuropean Association for Animal Production
Number109
ISSN (Print)0071-2477

Keywords

  • oncorhynchus-mykiss
  • identification

Cite this

Martin, S. A. M., Vilhelmsson, O. T., & Houlihan, D. F. J. (2004). Rainbow trout liver proteome: dietary manipulation and protein metabolism. In W. B. Souffrant, & C. C. Metges (Eds.), Progress in Research on Energy and Protein Metabolism : EAAP Scientific (Vol. 109, pp. 57-60). (FAO agricultural development paper. ; No. 109). Purdue University Press.