RANDOMLY AMPLIFIED POLYMORPHIC DNA ANALYSIS OF CLONAL POPULATION-STRUCTURE AND GEOGRAPHIC-VARIATION IN A FRESH-WATER BRYOZOAN

B OKAMURA, Catherine Sue Jones, Leslie Robert Noble

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The randomly amplified polymorphic DNA (RAPD) assay was used to identify genetic polymorphisms in three clonal populations of the freshwater bryozoan, Cristatella mucedo, a species with few useful biochemical genetic markers. Of the 19 decamer oligonucleotide primers screened, 13 gave clear, reproducible RAPD profiles. Clonal population structure was evident, and one clone was dominant at each site. Cluster analysis grouped populations from more distant localities separately (Thames Valley and Norfolk), whereas populations from the Thames Valley clustered together. However, even at the regional scale a high degree of relatedness pertained. This work is one of the first RAPD studies of natural populations, and demonstrates the suitability of the technique for examining population structure and geographic variation in clonal taxa.

Original languageEnglish
Pages (from-to)147-154
Number of pages8
JournalProceedings of the Royal Society of London. B, Biological Sciences
Volume253
Issue number1337
Publication statusPublished - 23 Aug 1993

Keywords

  • GENETIC-VARIATION
  • ARBITRARY PRIMERS
  • PCR
  • DIFFERENTIATION
  • DIVERSITY
  • SELECTION
  • ECOLOGY
  • APHIDS
  • SEX

Cite this

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title = "RANDOMLY AMPLIFIED POLYMORPHIC DNA ANALYSIS OF CLONAL POPULATION-STRUCTURE AND GEOGRAPHIC-VARIATION IN A FRESH-WATER BRYOZOAN",
abstract = "The randomly amplified polymorphic DNA (RAPD) assay was used to identify genetic polymorphisms in three clonal populations of the freshwater bryozoan, Cristatella mucedo, a species with few useful biochemical genetic markers. Of the 19 decamer oligonucleotide primers screened, 13 gave clear, reproducible RAPD profiles. Clonal population structure was evident, and one clone was dominant at each site. Cluster analysis grouped populations from more distant localities separately (Thames Valley and Norfolk), whereas populations from the Thames Valley clustered together. However, even at the regional scale a high degree of relatedness pertained. This work is one of the first RAPD studies of natural populations, and demonstrates the suitability of the technique for examining population structure and geographic variation in clonal taxa.",
keywords = "GENETIC-VARIATION, ARBITRARY PRIMERS, PCR, DIFFERENTIATION, DIVERSITY, SELECTION, ECOLOGY, APHIDS, SEX",
author = "B OKAMURA and Jones, {Catherine Sue} and Noble, {Leslie Robert}",
year = "1993",
month = "8",
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language = "English",
volume = "253",
pages = "147--154",
journal = "Proceedings of the Royal Society of London. B, Biological Sciences",
issn = "0962-8452",
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number = "1337",

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T1 - RANDOMLY AMPLIFIED POLYMORPHIC DNA ANALYSIS OF CLONAL POPULATION-STRUCTURE AND GEOGRAPHIC-VARIATION IN A FRESH-WATER BRYOZOAN

AU - OKAMURA, B

AU - Jones, Catherine Sue

AU - Noble, Leslie Robert

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Y1 - 1993/8/23

N2 - The randomly amplified polymorphic DNA (RAPD) assay was used to identify genetic polymorphisms in three clonal populations of the freshwater bryozoan, Cristatella mucedo, a species with few useful biochemical genetic markers. Of the 19 decamer oligonucleotide primers screened, 13 gave clear, reproducible RAPD profiles. Clonal population structure was evident, and one clone was dominant at each site. Cluster analysis grouped populations from more distant localities separately (Thames Valley and Norfolk), whereas populations from the Thames Valley clustered together. However, even at the regional scale a high degree of relatedness pertained. This work is one of the first RAPD studies of natural populations, and demonstrates the suitability of the technique for examining population structure and geographic variation in clonal taxa.

AB - The randomly amplified polymorphic DNA (RAPD) assay was used to identify genetic polymorphisms in three clonal populations of the freshwater bryozoan, Cristatella mucedo, a species with few useful biochemical genetic markers. Of the 19 decamer oligonucleotide primers screened, 13 gave clear, reproducible RAPD profiles. Clonal population structure was evident, and one clone was dominant at each site. Cluster analysis grouped populations from more distant localities separately (Thames Valley and Norfolk), whereas populations from the Thames Valley clustered together. However, even at the regional scale a high degree of relatedness pertained. This work is one of the first RAPD studies of natural populations, and demonstrates the suitability of the technique for examining population structure and geographic variation in clonal taxa.

KW - GENETIC-VARIATION

KW - ARBITRARY PRIMERS

KW - PCR

KW - DIFFERENTIATION

KW - DIVERSITY

KW - SELECTION

KW - ECOLOGY

KW - APHIDS

KW - SEX

M3 - Article

VL - 253

SP - 147

EP - 154

JO - Proceedings of the Royal Society of London. B, Biological Sciences

JF - Proceedings of the Royal Society of London. B, Biological Sciences

SN - 0962-8452

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