Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates

Gustavo Bravo Ruiz; Zoe K. Ross; Silke Schelenz; Neil A. R. Gow; Alexander Lorenz

doi:10.1101/514745

Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates

Gustavo Bravo Ruiz, Zoe K. Ross, Silke Schelenz, Neil A. R. Gow, Alexander Lorenz (Corresponding Author)

Research output: Contribution to journal › Article

8 Citations (Scopus)

7 Downloads (Pure)

Abstract

Candida auris is a newly emerging pathogenic microbe, having been identified as a medically relevant fungus as recently as 2009. It is the most drug-resistant yeast species known to date and its emergence and population structure are unusual. Because of its recent emergence we are largely ignorant about fundamental aspects of its general biology, life cycle, and population dynamics. Here we report the karyotype variability of 26 C. auris strains representing the four main clades. We demonstrate that all strains are haploid and have a highly plastic karyotype containing five to seven chromosomes, which can undergo marked alterations within a short time-frame when the fungus is put under genotoxic, heat, or osmotic stress. No simple correlation was found between karyotype pattern, drug resistance, and clade affiliation indicating that karyotype heterogeneity is rapidly evolving. As with other Candida species, these marked karyotype differences between isolates are likely to have an important impact on pathogenic traits of C. auris.

Original language	English
Pages (from-to)	1217-1228
Number of pages	12
Journal	Current Genetics
Volume	65
Issue number	5
Early online date	24 Apr 2019
DOIs	https://doi.org/10.1101/514745 https://doi.org/10.1007/s00294-019-00976-w
Publication status	Published - Oct 2019

Keywords

Candida auris
chromosome number
chromosome size
genome size
rRNA genes
Genome size
Chromosome size
Chromosome number
Karyotype evolution
PHENOTYPIC VARIATION
DE-NOVO
NUMBER
INSTABILITY
MECHANISM
GENOME
ANEUPLOIDY
MUTANTS
RIBOSOMAL DNA
ALBICANS

ASJC Scopus subject areas

Genetics

Access to Document

10.1101/514745Licence: CC BY-NC-ND
10.1007/s00294-019-00976-wLicence: CC BY

Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates
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BravoRuiz2019_Article_RapidAndExtensiveKaryotypeDive_VOR
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Profiles

Alexander Lorenz

Person: Academic

Equipment

Iain Fraser Cytometry Centre

Andrea Holme (Manager), Linda Duncan (Senior Application Scientist), Ailsa Laird (Technician) & Kate Burgoyne (Technician)

Institute of Medical Sciences

Research Facilities: Facility

Cite this

Bravo Ruiz, G., Ross, Z. K., Schelenz, S., Gow, N. A. R., & Lorenz, A. (2019). Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates. Current Genetics, 65(5), 1217-1228. https://doi.org/10.1101/514745, https://doi.org/10.1007/s00294-019-00976-w

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title = "Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates",

abstract = "Candida auris is a newly emerging pathogenic microbe, having been identified as a medically relevant fungus as recently as 2009. It is the most drug-resistant yeast species known to date and its emergence and population structure are unusual. Because of its recent emergence we are largely ignorant about fundamental aspects of its general biology, life cycle, and population dynamics. Here we report the karyotype variability of 26 C. auris strains representing the four main clades. We demonstrate that all strains are haploid and have a highly plastic karyotype containing five to seven chromosomes, which can undergo marked alterations within a short time-frame when the fungus is put under genotoxic, heat, or osmotic stress. No simple correlation was found between karyotype pattern, drug resistance, and clade affiliation indicating that karyotype heterogeneity is rapidly evolving. As with other Candida species, these marked karyotype differences between isolates are likely to have an important impact on pathogenic traits of C. auris.",

keywords = "Candida auris, chromosome number, chromosome size, genome size, rRNA genes, Genome size, Chromosome size, Chromosome number, Karyotype evolution, PHENOTYPIC VARIATION, DE-NOVO, NUMBER, INSTABILITY, MECHANISM, GENOME, ANEUPLOIDY, MUTANTS, RIBOSOMAL DNA, ALBICANS",

author = "{Bravo Ruiz}, Gustavo and Ross, {Zoe K.} and Silke Schelenz and Gow, {Neil A. R.} and Alexander Lorenz",

note = "Open access via the Springer Compact Agreement. We are grateful to Arunaloke Chakrabarti, Anuradha Chowdhary, Elizabeth Johnson (PHE), Takashi Kubota, and Shawn Lockhart (CDC) for providing strains. Flow cytometry was performed at the Iain Fraser Cytometry Centre (IFCC), University of Aberdeen (Raif Yuecel). This work was funded by the Medical Research Council (MRC) Centre for Medical Mycology at the University of Aberdeen (MR/P501955/1), a Wellcome Trust Institutional Strategic Support Fund grant awarded to the University of Aberdeen (204815/Z/16/Z), a Tenovus Scotland project grant (G17.02), a Royal Society Research Grant (RG140254) to AL, and Wellcome Trust Strategic Award, Senior Investigator and Collaborative Awards (080088, 086827, 075470, 099215, and 097377) to NARG. ",

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AU - Schelenz, Silke

AU - Gow, Neil A. R.

AU - Lorenz, Alexander

N1 - Open access via the Springer Compact Agreement. We are grateful to Arunaloke Chakrabarti, Anuradha Chowdhary, Elizabeth Johnson (PHE), Takashi Kubota, and Shawn Lockhart (CDC) for providing strains. Flow cytometry was performed at the Iain Fraser Cytometry Centre (IFCC), University of Aberdeen (Raif Yuecel). This work was funded by the Medical Research Council (MRC) Centre for Medical Mycology at the University of Aberdeen (MR/P501955/1), a Wellcome Trust Institutional Strategic Support Fund grant awarded to the University of Aberdeen (204815/Z/16/Z), a Tenovus Scotland project grant (G17.02), a Royal Society Research Grant (RG140254) to AL, and Wellcome Trust Strategic Award, Senior Investigator and Collaborative Awards (080088, 086827, 075470, 099215, and 097377) to NARG.

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N2 - Candida auris is a newly emerging pathogenic microbe, having been identified as a medically relevant fungus as recently as 2009. It is the most drug-resistant yeast species known to date and its emergence and population structure are unusual. Because of its recent emergence we are largely ignorant about fundamental aspects of its general biology, life cycle, and population dynamics. Here we report the karyotype variability of 26 C. auris strains representing the four main clades. We demonstrate that all strains are haploid and have a highly plastic karyotype containing five to seven chromosomes, which can undergo marked alterations within a short time-frame when the fungus is put under genotoxic, heat, or osmotic stress. No simple correlation was found between karyotype pattern, drug resistance, and clade affiliation indicating that karyotype heterogeneity is rapidly evolving. As with other Candida species, these marked karyotype differences between isolates are likely to have an important impact on pathogenic traits of C. auris.

AB - Candida auris is a newly emerging pathogenic microbe, having been identified as a medically relevant fungus as recently as 2009. It is the most drug-resistant yeast species known to date and its emergence and population structure are unusual. Because of its recent emergence we are largely ignorant about fundamental aspects of its general biology, life cycle, and population dynamics. Here we report the karyotype variability of 26 C. auris strains representing the four main clades. We demonstrate that all strains are haploid and have a highly plastic karyotype containing five to seven chromosomes, which can undergo marked alterations within a short time-frame when the fungus is put under genotoxic, heat, or osmotic stress. No simple correlation was found between karyotype pattern, drug resistance, and clade affiliation indicating that karyotype heterogeneity is rapidly evolving. As with other Candida species, these marked karyotype differences between isolates are likely to have an important impact on pathogenic traits of C. auris.

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