Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates

Gustavo Bravo Ruiz, Zoe K. Ross, Silke Schelenz, Neil A. R. Gow, Alexander Lorenz (Corresponding Author)

Research output: Contribution to journalArticle

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Abstract

Candida auris is a newly emerging pathogenic microbe, having been identified as a medically relevant fungus as recently as 2009. It is the most drug-resistant yeast species known to date and its emergence and population structure are unusual. Because of its recent emergence we are largely ignorant about fundamental aspects of its general biology, life cycle, and population dynamics. Here we report the karyotype variability of 26 C. auris strains representing the four main clades. We demonstrate that all strains are haploid and have a highly plastic karyotype containing five to seven chromosomes, which can undergo marked alterations within a short time-frame when the fungus is put under genotoxic, heat, or osmotic stress. No simple correlation was found between karyotype pattern, drug resistance, and clade affiliation indicating that karyotype heterogeneity is rapidly evolving. As with other Candida species, these marked karyotype differences between isolates are likely to have an important impact on pathogenic traits of C. auris.
Original languageEnglish
Pages (from-to)1217-1228
Number of pages12
JournalCurrent Genetics
Volume65
Issue number5
Early online date24 Apr 2019
DOIs
Publication statusPublished - Oct 2019

Fingerprint

Haploidy
Karyotype
Candida
Fungi
Osmotic Pressure
Population Dynamics
Life Cycle Stages
Drug Resistance
Plastics
DNA Damage
Hot Temperature
Chromosomes
Yeasts
Pharmaceutical Preparations
Population

Keywords

  • Candida auris
  • chromosome number
  • chromosome size
  • genome size
  • rRNA genes
  • Genome size
  • Chromosome size
  • Chromosome number
  • Karyotype evolution

ASJC Scopus subject areas

  • Genetics

Cite this

Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates. / Bravo Ruiz, Gustavo; Ross, Zoe K.; Schelenz, Silke; Gow, Neil A. R.; Lorenz, Alexander (Corresponding Author).

In: Current Genetics, Vol. 65, No. 5, 10.2019, p. 1217-1228.

Research output: Contribution to journalArticle

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abstract = "Candida auris is a newly emerging pathogenic microbe, having been identified as a medically relevant fungus as recently as 2009. It is the most drug-resistant yeast species known to date and its emergence and population structure are unusual. Because of its recent emergence we are largely ignorant about fundamental aspects of its general biology, life cycle, and population dynamics. Here we report the karyotype variability of 26 C. auris strains representing the four main clades. We demonstrate that all strains are haploid and have a highly plastic karyotype containing five to seven chromosomes, which can undergo marked alterations within a short time-frame when the fungus is put under genotoxic, heat, or osmotic stress. No simple correlation was found between karyotype pattern, drug resistance, and clade affiliation indicating that karyotype heterogeneity is rapidly evolving. As with other Candida species, these marked karyotype differences between isolates are likely to have an important impact on pathogenic traits of C. auris.",
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