Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1217-1228 |
| Journal | Current Genetics |
| Volume | 65 |
| Issue number | 5 |
| Early online date | 24 Apr 2019 |
| DOIs | |
| Publication status | Published - Oct 2019 |
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Keywords
- Candida auris
- chromosome number
- chromosome size
- genome size
- rRNA genes
- Genome size
- Chromosome size
- Chromosome number
- Karyotype evolution
- PHENOTYPIC VARIATION
- DE-NOVO
- NUMBER
- INSTABILITY
- MECHANISM
- GENOME
- ANEUPLOIDY
- MUTANTS
- RIBOSOMAL DNA
- ALBICANS
ASJC Scopus subject areas
- Genetics
Cite this
Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates. / Bravo Ruiz, Gustavo; Ross, Zoe K.; Schelenz, Silke; Gow, Neil A. R.; Lorenz, Alexander (Corresponding Author).
In: Current Genetics, Vol. 65, No. 5, 10.2019, p. 1217-1228.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates
AU - Bravo Ruiz, Gustavo
AU - Ross, Zoe K.
AU - Schelenz, Silke
AU - Gow, Neil A. R.
AU - Lorenz, Alexander
N1 - Open access via the Springer Compact Agreement. We are grateful to Arunaloke Chakrabarti, Anuradha Chowdhary, Elizabeth Johnson (PHE), Takashi Kubota, and Shawn Lockhart (CDC) for providing strains. Flow cytometry was performed at the Iain Fraser Cytometry Centre (IFCC), University of Aberdeen (Raif Yuecel). This work was funded by the Medical Research Council (MRC) Centre for Medical Mycology at the University of Aberdeen (MR/P501955/1), a Wellcome Trust Institutional Strategic Support Fund grant awarded to the University of Aberdeen (204815/Z/16/Z), a Tenovus Scotland project grant (G17.02), a Royal Society Research Grant (RG140254) to AL, and Wellcome Trust Strategic Award, Senior Investigator and Collaborative Awards (080088, 086827, 075470, 099215, and 097377) to NARG.
PY - 2019/10
Y1 - 2019/10
N2 - Candida auris is a newly emerging pathogenic microbe, having been identified as a medically relevant fungus as recently as 2009. It is the most drug-resistant yeast species known to date and its emergence and population structure are unusual. Because of its recent emergence we are largely ignorant about fundamental aspects of its general biology, life cycle, and population dynamics. Here we report the karyotype variability of 26 C. auris strains representing the four main clades. We demonstrate that all strains are haploid and have a highly plastic karyotype containing five to seven chromosomes, which can undergo marked alterations within a short time-frame when the fungus is put under genotoxic, heat, or osmotic stress. No simple correlation was found between karyotype pattern, drug resistance, and clade affiliation indicating that karyotype heterogeneity is rapidly evolving. As with other Candida species, these marked karyotype differences between isolates are likely to have an important impact on pathogenic traits of C. auris.
AB - Candida auris is a newly emerging pathogenic microbe, having been identified as a medically relevant fungus as recently as 2009. It is the most drug-resistant yeast species known to date and its emergence and population structure are unusual. Because of its recent emergence we are largely ignorant about fundamental aspects of its general biology, life cycle, and population dynamics. Here we report the karyotype variability of 26 C. auris strains representing the four main clades. We demonstrate that all strains are haploid and have a highly plastic karyotype containing five to seven chromosomes, which can undergo marked alterations within a short time-frame when the fungus is put under genotoxic, heat, or osmotic stress. No simple correlation was found between karyotype pattern, drug resistance, and clade affiliation indicating that karyotype heterogeneity is rapidly evolving. As with other Candida species, these marked karyotype differences between isolates are likely to have an important impact on pathogenic traits of C. auris.
KW - Candida auris
KW - chromosome number
KW - chromosome size
KW - genome size
KW - rRNA genes
KW - Genome size
KW - Chromosome size
KW - Chromosome number
KW - Karyotype evolution
KW - PHENOTYPIC VARIATION
KW - DE-NOVO
KW - NUMBER
KW - INSTABILITY
KW - MECHANISM
KW - GENOME
KW - ANEUPLOIDY
KW - MUTANTS
KW - RIBOSOMAL DNA
KW - ALBICANS
UR - https://www.biorxiv.org/content/10.1101/514745v1
UR - http://www.mendeley.com/research/rapid-extensive-karyotype-diversification-haploid-clinical-candida-auris-isolates
UR - http://www.scopus.com/inward/record.url?scp=85064813898&partnerID=8YFLogxK
UR - https://abdn.pure.elsevier.com/en/en/researchoutput/rapid-and-extensive-karyotype-diversification-in-haploid-clinical-candida-auris-isolates(97c2faef-7bba-4a0a-be0e-87f5b1916f45).html
U2 - 10.1101/514745
DO - 10.1101/514745
M3 - Article
C2 - 31020384
VL - 65
SP - 1217
EP - 1228
JO - Current Genetics
JF - Current Genetics
SN - 0172-8083
IS - 5
ER -