Rapid detection of aneuploidy following the generation of mutants in Candida albicans

Megan D Lenardon, André Nantel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Techniques used to generate mutants in Candida albicans commonly result in additional and undesired genetic rearrangements. Detection of aneuploidy is, therefore, an important step forward in the quality control of mutant phenotypes. In this chapter, we describe how to extract genomic DNA and perform a quantitative multiplex PCR to compare the karyotype of any mutant strain to that of its parent and allow the detection of any unwanted aneuploidy.
Original languageEnglish
Pages (from-to)41-49
Number of pages9
JournalMethods in Molecular Biology
Volume845
Issue number1
DOIs
Publication statusPublished - 2012

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Aneuploidy
Candida albicans
Multiplex Polymerase Chain Reaction
Karyotype
Quality Control
Phenotype
DNA

Cite this

Rapid detection of aneuploidy following the generation of mutants in Candida albicans. / Lenardon, Megan D; Nantel, André.

In: Methods in Molecular Biology, Vol. 845, No. 1, 2012, p. 41-49.

Research output: Contribution to journalArticle

Lenardon, Megan D ; Nantel, André. / Rapid detection of aneuploidy following the generation of mutants in Candida albicans. In: Methods in Molecular Biology. 2012 ; Vol. 845, No. 1. pp. 41-49.
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