Rapid detection of aneuploidy following the generation of mutants in Candida albicans

Megan D Lenardon; André Nantel

doi:10.1007/978-1-61779-539-8_3

Rapid detection of aneuploidy following the generation of mutants in Candida albicans

Megan D Lenardon, André Nantel

Medical Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Techniques used to generate mutants in Candida albicans commonly result in additional and undesired genetic rearrangements. Detection of aneuploidy is, therefore, an important step forward in the quality control of mutant phenotypes. In this chapter, we describe how to extract genomic DNA and perform a quantitative multiplex PCR to compare the karyotype of any mutant strain to that of its parent and allow the detection of any unwanted aneuploidy.

Original language	English
Pages (from-to)	41-49
Number of pages	9
Journal	Methods in Molecular Biology
Volume	845
Issue number	1
DOIs	https://doi.org/10.1007/978-1-61779-539-8_3
Publication status	Published - 2012

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title = "Rapid detection of aneuploidy following the generation of mutants in Candida albicans",

abstract = "Techniques used to generate mutants in Candida albicans commonly result in additional and undesired genetic rearrangements. Detection of aneuploidy is, therefore, an important step forward in the quality control of mutant phenotypes. In this chapter, we describe how to extract genomic DNA and perform a quantitative multiplex PCR to compare the karyotype of any mutant strain to that of its parent and allow the detection of any unwanted aneuploidy.",

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AB - Techniques used to generate mutants in Candida albicans commonly result in additional and undesired genetic rearrangements. Detection of aneuploidy is, therefore, an important step forward in the quality control of mutant phenotypes. In this chapter, we describe how to extract genomic DNA and perform a quantitative multiplex PCR to compare the karyotype of any mutant strain to that of its parent and allow the detection of any unwanted aneuploidy.

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Rapid detection of aneuploidy following the generation of mutants in Candida albicans

Abstract

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