TY - JOUR
T1 - Rapid High-Performance Liquid-Chromatographic Method for the Quantitation of Polyamines as their Dansyl Derivatives
T2 - application to plant and animal tissues
AU - MARCE, M
AU - Brown, David Stanley
AU - CAPELL, T
AU - FIGUERAS, X
AU - TIBURCIO, A F
PY - 1995/4/21
Y1 - 1995/4/21
N2 - A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.
AB - A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.
KW - precolumn derivatization
KW - separation
KW - amines
U2 - 10.1016/0378-4347(94)00586-T
DO - 10.1016/0378-4347(94)00586-T
M3 - Comment/debate
SN - 1572-6495
VL - 666
SP - 329
EP - 335
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 2
ER -