Rapid High-Performance Liquid-Chromatographic Method for the Quantitation of Polyamines as their Dansyl Derivatives: application to plant and animal tissues

M MARCE, David Stanley Brown, T CAPELL, X FIGUERAS, A F TIBURCIO

    Research output: Contribution to journalComment/debate

    119 Citations (Scopus)

    Abstract

    A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.

    Original languageEnglish
    Pages (from-to)329-335
    Number of pages7
    JournalJournal of Chromatography B: Biomedical Sciences and Applications
    Volume666
    Issue number2
    DOIs
    Publication statusPublished - 21 Apr 1995

    Keywords

    • precolumn derivatization
    • separation
    • amines

    Cite this

    Rapid High-Performance Liquid-Chromatographic Method for the Quantitation of Polyamines as their Dansyl Derivatives : application to plant and animal tissues . / MARCE, M ; Brown, David Stanley; CAPELL, T ; FIGUERAS, X ; TIBURCIO, A F .

    In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 666, No. 2, 21.04.1995, p. 329-335.

    Research output: Contribution to journalComment/debate

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    title = "Rapid High-Performance Liquid-Chromatographic Method for the Quantitation of Polyamines as their Dansyl Derivatives: application to plant and animal tissues",
    abstract = "A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.",
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    AU - MARCE, M

    AU - Brown, David Stanley

    AU - CAPELL, T

    AU - FIGUERAS, X

    AU - TIBURCIO, A F

    PY - 1995/4/21

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    N2 - A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.

    AB - A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.

    KW - precolumn derivatization

    KW - separation

    KW - amines

    U2 - 10.1016/0378-4347(94)00586-T

    DO - 10.1016/0378-4347(94)00586-T

    M3 - Comment/debate

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