Rapid High-Performance Liquid-Chromatographic Method for the Quantitation of Polyamines as their Dansyl Derivatives: application to plant and animal tissues

M  MARCE; David Stanley Brown; T  CAPELL; X  FIGUERAS; A F  TIBURCIO

doi:10.1016/0378-4347(94)00586-T

Rapid High-Performance Liquid-Chromatographic Method for the Quantitation of Polyamines as their Dansyl Derivatives: application to plant and animal tissues

M MARCE, David Stanley Brown, T CAPELL, X FIGUERAS, A F TIBURCIO

Research output: Contribution to journal › Comment/debate › peer-review

133 Citations (Scopus)

Abstract

A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.

Original language	English
Pages (from-to)	329-335
Number of pages	7
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	666
Issue number	2
DOIs	https://doi.org/10.1016/0378-4347(94)00586-T
Publication status	Published - 21 Apr 1995

Keywords

precolumn derivatization
separation
amines

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10.1016/0378-4347(94)00586-T

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Rapid High-Performance Liquid-Chromatographic Method for the Quantitation of Polyamines as their Dansyl Derivatives : application to plant and animal tissues . / MARCE, M ; Brown, David Stanley; CAPELL, T et al.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 666, No. 2, 21.04.1995, p. 329-335.

Research output: Contribution to journal › Comment/debate › peer-review

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title = "Rapid High-Performance Liquid-Chromatographic Method for the Quantitation of Polyamines as their Dansyl Derivatives: application to plant and animal tissues ",

abstract = "A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.",

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AU - TIBURCIO, A F

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AB - A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.

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KW - separation

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DO - 10.1016/0378-4347(94)00586-T

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SN - 1570-0232

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ER -

Rapid High-Performance Liquid-Chromatographic Method for the Quantitation of Polyamines as their Dansyl Derivatives: application to plant and animal tissues

Abstract

Keywords

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