Rapid High-Performance Liquid-Chromatographic Method for the Quantitation of Polyamines as their Dansyl Derivatives: application to plant and animal tissues

M MARCE, David Stanley Brown, T CAPELL, X FIGUERAS, A F TIBURCIO

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    Abstract

    A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.

    Original languageEnglish
    Pages (from-to)329-335
    Number of pages7
    JournalJournal of Chromatography B: Biomedical Sciences and Applications
    Volume666
    Issue number2
    DOIs
    Publication statusPublished - 21 Apr 1995

    Keywords

    • precolumn derivatization
    • separation
    • amines

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