Rapid method for quantification of anthocyanidins and anthocyanins in human biological samples

Deepak M. Kasote (Corresponding Author), Gary J. Duncan, Madalina Neacsu, Wendy R. Russell (Corresponding Author)

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Using simple solvent extraction and enzymatic hydrolysis, a rapid LC-MS/MS method for quantification of free and conjugated forms of anthocyanidins and anthocyanins in plasma and urine samples was developed and validated. A mixed enzymatic treatment containing β-glucuronidase (100 U mL −1 ) and sulfatase (2.5 U mL −1 ) for 5 min (37 °C; pH 6) was optimal condition for deconjugation of anthocyanidins and anthocyanins in urine and plasma samples. The LC–MS/MS allowed quantifying thirteen different anthocyanidins and anthocyanins simultaneously. The developed LC–MS/MS method was precise and accurate over multiple days and nominal concentrations. The stability assessment study confirmed that the long-term storage and/or periodic use of plasma and urine samples might have a considerable impact on the stability of some anthocyanidins. The method was successfully applied to measure anthocyanidins and anthocyanins in plasma and urine samples following consumption of acute blueberry test meals.

Original languageEnglish
Pages (from-to)56-63
Number of pages8
JournalFood Chemistry
Volume290
Early online date21 Mar 2019
DOIs
Publication statusPublished - 30 Aug 2019

Fingerprint

anthocyanidins
Anthocyanins
rapid methods
anthocyanins
urine
sampling
Urine
Plasmas
arylsulfatase
test meals
enzymatic treatment
blueberries
enzymatic hydrolysis
Blueberry Plants
methodology
Sulfatases
Enzymatic hydrolysis
Glucuronidase
Solvent extraction
Meals

Keywords

  • Acute blueberry consumption
  • Anthocyanidin
  • Anthocyanin
  • Plasma
  • Urine
  • MASS-SPECTROMETRY METHOD
  • LIQUID-CHROMATOGRAPHY
  • HUMAN PLASMA
  • URINE
  • VALIDATION
  • BIOAVAILABILITY

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Cite this

Rapid method for quantification of anthocyanidins and anthocyanins in human biological samples. / Kasote, Deepak M. (Corresponding Author); Duncan, Gary J.; Neacsu, Madalina; Russell, Wendy R. (Corresponding Author).

In: Food Chemistry, Vol. 290, 30.08.2019, p. 56-63.

Research output: Contribution to journalArticle

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