Rapid phenotypic evolution in multidrug-resistant Klebsiella pneumoniae hospital outbreak strains

Lucy van Dorp (Corresponding Author), Qi Wang, Liam P. Shaw, Mislav Acman, Ola B. Brynildsrud, Vegard Eldholm, Ruobing Wang, Hua Gao, Yuyao Yin, Hongbin Chen, Chuling Ding, Rhys A. Farrer, Xavier Didelot, Francois Balloux (Corresponding Author), Hui Wang (Corresponding Author)

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Abstract

Carbapenem-resistant Klebsiella pneumoniae (CRKP) increasingly cause high-mortality outbreaks in hospital settings globally. Following a patient fatality at a hospital in Beijing due to a bla KPC-2-positive CRKP infection, close monitoring was put in place over the course of 14 months to characterize all bla KPC-2-positive CRKP in circulation in the hospital. Whole genome sequences were generated for 100 isolates from bla KPC-2-positive isolates from infected patients, carriers and the hospital environment. Phylogenetic analyses identified a closely related cluster of 82 sequence type 11 (ST11) isolates circulating in the hospital for at least a year prior to admission of the index patient. The majority of inferred transmissions for these isolates involved patients in intensive care units. Whilst the 82 ST11 isolates collected during the surveillance effort all had closely related chromosomes, we observed extensive diversity in their antimicrobial resistance (AMR) phenotypes. We were able to reconstruct the major genomic changes underpinning this variation in AMR profiles, including multiple gains and losses of entire plasmids and recombination events between plasmids, including transposition of bla KPC-2. We also identified specific cases where variation in plasmid copy number correlated with the level of phenotypic resistance to drugs, suggesting that the number of resistance elements carried by a strain may play a role in determining the level of AMR. Our findings highlight the epidemiological value of whole genome sequencing for investigating multi-drug-resistant hospital infections and illustrate that standard typing schemes cannot capture the extraordinarily fast genome evolution of CRKP isolates.
Original languageEnglish
Number of pages11
JournalMicrobial Genomics
Volume5
Early online date2 Apr 2019
DOIs
Publication statusPublished - 2019

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Carbapenems
Klebsiella pneumoniae
Disease Outbreaks
Plasmids
Genome
Klebsiella Infections
Patient Admission
Cross Infection
Hospital Mortality
Drug Resistance
Genetic Recombination
Intensive Care Units
Chromosomes
Phenotype
Pharmaceutical Preparations

Keywords

  • nosocomial pathogens
  • antimicrobial resistance
  • carbapenem-resistant Klebsiella pneumoniae (CRKP)
  • horizontal gene transfer
  • plasmids
  • transmission chains
  • PLASMIDS
  • ST11
  • ANTIMICROBIAL RESISTANCE
  • CLONE
  • GENOME
  • ISOLATE
  • DISSEMINATION
  • EPIDEMIOLOGY
  • CARBAPENEM RESISTANCE
  • KPC

ASJC Scopus subject areas

  • Medicine(all)

Cite this

van Dorp, L., Wang, Q., Shaw, L. P., Acman, M., Brynildsrud, O. B., Eldholm, V., ... Wang, H. (2019). Rapid phenotypic evolution in multidrug-resistant Klebsiella pneumoniae hospital outbreak strains. Microbial Genomics, 5. https://doi.org/10.1099/mgen.0.000263

Rapid phenotypic evolution in multidrug-resistant Klebsiella pneumoniae hospital outbreak strains. / van Dorp, Lucy (Corresponding Author); Wang, Qi; Shaw, Liam P.; Acman, Mislav; Brynildsrud, Ola B.; Eldholm, Vegard; Wang, Ruobing; Gao, Hua; Yin, Yuyao; Chen, Hongbin; Ding, Chuling; Farrer, Rhys A.; Didelot, Xavier; Balloux, Francois (Corresponding Author); Wang, Hui (Corresponding Author).

In: Microbial Genomics, Vol. 5, 2019.

Research output: Contribution to journalArticle

van Dorp, L, Wang, Q, Shaw, LP, Acman, M, Brynildsrud, OB, Eldholm, V, Wang, R, Gao, H, Yin, Y, Chen, H, Ding, C, Farrer, RA, Didelot, X, Balloux, F & Wang, H 2019, 'Rapid phenotypic evolution in multidrug-resistant Klebsiella pneumoniae hospital outbreak strains' Microbial Genomics, vol. 5. https://doi.org/10.1099/mgen.0.000263
van Dorp, Lucy ; Wang, Qi ; Shaw, Liam P. ; Acman, Mislav ; Brynildsrud, Ola B. ; Eldholm, Vegard ; Wang, Ruobing ; Gao, Hua ; Yin, Yuyao ; Chen, Hongbin ; Ding, Chuling ; Farrer, Rhys A. ; Didelot, Xavier ; Balloux, Francois ; Wang, Hui. / Rapid phenotypic evolution in multidrug-resistant Klebsiella pneumoniae hospital outbreak strains. In: Microbial Genomics. 2019 ; Vol. 5.
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abstract = "Carbapenem-resistant Klebsiella pneumoniae (CRKP) increasingly cause high-mortality outbreaks in hospital settings globally. Following a patient fatality at a hospital in Beijing due to a bla KPC-2-positive CRKP infection, close monitoring was put in place over the course of 14 months to characterize all bla KPC-2-positive CRKP in circulation in the hospital. Whole genome sequences were generated for 100 isolates from bla KPC-2-positive isolates from infected patients, carriers and the hospital environment. Phylogenetic analyses identified a closely related cluster of 82 sequence type 11 (ST11) isolates circulating in the hospital for at least a year prior to admission of the index patient. The majority of inferred transmissions for these isolates involved patients in intensive care units. Whilst the 82 ST11 isolates collected during the surveillance effort all had closely related chromosomes, we observed extensive diversity in their antimicrobial resistance (AMR) phenotypes. We were able to reconstruct the major genomic changes underpinning this variation in AMR profiles, including multiple gains and losses of entire plasmids and recombination events between plasmids, including transposition of bla KPC-2. We also identified specific cases where variation in plasmid copy number correlated with the level of phenotypic resistance to drugs, suggesting that the number of resistance elements carried by a strain may play a role in determining the level of AMR. Our findings highlight the epidemiological value of whole genome sequencing for investigating multi-drug-resistant hospital infections and illustrate that standard typing schemes cannot capture the extraordinarily fast genome evolution of CRKP isolates.",
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note = "L. v. D., L. P. S., X. D., H. W. and F. B. acknowledge financial support from the Newton Trust UK–China NSFC initiative (grants MR/P007597/1 and 81661138006). F. B. acknowledges support from the BBSRC GCRF scheme. H. W. additionally acknowledges support from China NSFC grant 81625014. H. C. acknowledges financial support from a 111 Talent Discipline Planning of PKUPH award for a 1-year visit at University College London. Data statement: All supporting data, code and protocols have been provided within the article or through supplementary data files. Nine supplementary tables and fifteen supplementary figures are available with the online version of this article.",
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AU - Eldholm, Vegard

AU - Wang, Ruobing

AU - Gao, Hua

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AU - Chen, Hongbin

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AU - Balloux, Francois

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N1 - L. v. D., L. P. S., X. D., H. W. and F. B. acknowledge financial support from the Newton Trust UK–China NSFC initiative (grants MR/P007597/1 and 81661138006). F. B. acknowledges support from the BBSRC GCRF scheme. H. W. additionally acknowledges support from China NSFC grant 81625014. H. C. acknowledges financial support from a 111 Talent Discipline Planning of PKUPH award for a 1-year visit at University College London. Data statement: All supporting data, code and protocols have been provided within the article or through supplementary data files. Nine supplementary tables and fifteen supplementary figures are available with the online version of this article.

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