Rapid Quantification of Aortic Lesions in ApoE(-/-) Mice

John H Beattie, Susan J Duthie, In-Sook Kwun, Tae-Youl Ha, Margaret J Gordon

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    The quantification of aortic lesions is an important endpoint analysis for evaluating atherogenesis in mouse models of atherosclerosis. Morphometric methods involving the staining of aorta with a Sudan lysochrome followed by image analysis of the stained lesion area are commonly used. We have developed a more rapid method involving solubilisation of the stain retained by aortic lesions. In 2 separate studies, 5-week-old male apoE(-/-) and C57BL/6 wild-type (apoE(+/+)) mice were given a high fat (21%), Western-type diet for 13, 15 or 25 weeks. At study termination, the descending thoracic aorta (DA) and/or aortic arch (AA) were stained with Oil Red O (ORO). The incorporated stain was extracted using chloroform/methanol (2:1) solvent and quantified by spectrophotometry at 520 nm. In study 1 (13 weeks), ORO stain in the AA and DA of apoE(-/-) mice was 1.9 and 1.4 times higher than background staining of apoE(+/+) aorta tissue, respectively. At 15 and 25 weeks (study 2), ORO stain in the AA of apoE(-/-) mice was 1.9 and 2.5 times higher than the background, respectively. We conclude that the ORO solubilisation technique applied to AA samples is a very useful and rapid method for atherosclerotic lesion quantification. Copyright (C) 2009 S. Karger AG, Basel

    Original languageEnglish
    Pages (from-to)347-352
    Number of pages6
    JournalJournal of Vascular Research
    Volume46
    Issue number4
    Early online date10 Jan 2009
    DOIs
    Publication statusPublished - Jun 2009

    Keywords

    • atherosclerosis
    • atherosclerotic lesions
    • apoE(-/-) mice
    • plaque quantification
    • lysochrome
    • oil red o
    • mouse models
    • apolipoprotein-E
    • deficient mice
    • apoe
    • apoE–/– mice

    Cite this

    Rapid Quantification of Aortic Lesions in ApoE(-/-) Mice. / Beattie, John H; Duthie, Susan J; Kwun, In-Sook; Ha, Tae-Youl; Gordon, Margaret J.

    In: Journal of Vascular Research, Vol. 46, No. 4, 06.2009, p. 347-352.

    Research output: Contribution to journalArticle

    Beattie, JH, Duthie, SJ, Kwun, I-S, Ha, T-Y & Gordon, MJ 2009, 'Rapid Quantification of Aortic Lesions in ApoE(-/-) Mice', Journal of Vascular Research, vol. 46, no. 4, pp. 347-352. https://doi.org/10.1159/000189795
    Beattie, John H ; Duthie, Susan J ; Kwun, In-Sook ; Ha, Tae-Youl ; Gordon, Margaret J. / Rapid Quantification of Aortic Lesions in ApoE(-/-) Mice. In: Journal of Vascular Research. 2009 ; Vol. 46, No. 4. pp. 347-352.
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    abstract = "The quantification of aortic lesions is an important endpoint analysis for evaluating atherogenesis in mouse models of atherosclerosis. Morphometric methods involving the staining of aorta with a Sudan lysochrome followed by image analysis of the stained lesion area are commonly used. We have developed a more rapid method involving solubilisation of the stain retained by aortic lesions. In 2 separate studies, 5-week-old male apoE(-/-) and C57BL/6 wild-type (apoE(+/+)) mice were given a high fat (21{\%}), Western-type diet for 13, 15 or 25 weeks. At study termination, the descending thoracic aorta (DA) and/or aortic arch (AA) were stained with Oil Red O (ORO). The incorporated stain was extracted using chloroform/methanol (2:1) solvent and quantified by spectrophotometry at 520 nm. In study 1 (13 weeks), ORO stain in the AA and DA of apoE(-/-) mice was 1.9 and 1.4 times higher than background staining of apoE(+/+) aorta tissue, respectively. At 15 and 25 weeks (study 2), ORO stain in the AA of apoE(-/-) mice was 1.9 and 2.5 times higher than the background, respectively. We conclude that the ORO solubilisation technique applied to AA samples is a very useful and rapid method for atherosclerotic lesion quantification. Copyright (C) 2009 S. Karger AG, Basel",
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    AB - The quantification of aortic lesions is an important endpoint analysis for evaluating atherogenesis in mouse models of atherosclerosis. Morphometric methods involving the staining of aorta with a Sudan lysochrome followed by image analysis of the stained lesion area are commonly used. We have developed a more rapid method involving solubilisation of the stain retained by aortic lesions. In 2 separate studies, 5-week-old male apoE(-/-) and C57BL/6 wild-type (apoE(+/+)) mice were given a high fat (21%), Western-type diet for 13, 15 or 25 weeks. At study termination, the descending thoracic aorta (DA) and/or aortic arch (AA) were stained with Oil Red O (ORO). The incorporated stain was extracted using chloroform/methanol (2:1) solvent and quantified by spectrophotometry at 520 nm. In study 1 (13 weeks), ORO stain in the AA and DA of apoE(-/-) mice was 1.9 and 1.4 times higher than background staining of apoE(+/+) aorta tissue, respectively. At 15 and 25 weeks (study 2), ORO stain in the AA of apoE(-/-) mice was 1.9 and 2.5 times higher than the background, respectively. We conclude that the ORO solubilisation technique applied to AA samples is a very useful and rapid method for atherosclerotic lesion quantification. Copyright (C) 2009 S. Karger AG, Basel

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