Abstract
The quantification of aortic lesions is an important endpoint analysis for evaluating atherogenesis in mouse models of atherosclerosis. Morphometric methods involving the staining of aorta with a Sudan lysochrome followed by image analysis of the stained lesion area are commonly used. We have developed a more rapid method involving solubilisation of the stain retained by aortic lesions. In 2 separate studies, 5-week-old male apoE(-/-) and C57BL/6 wild-type (apoE(+/+)) mice were given a high fat (21%), Western-type diet for 13, 15 or 25 weeks. At study termination, the descending thoracic aorta (DA) and/or aortic arch (AA) were stained with Oil Red O (ORO). The incorporated stain was extracted using chloroform/methanol (2:1) solvent and quantified by spectrophotometry at 520 nm. In study 1 (13 weeks), ORO stain in the AA and DA of apoE(-/-) mice was 1.9 and 1.4 times higher than background staining of apoE(+/+) aorta tissue, respectively. At 15 and 25 weeks (study 2), ORO stain in the AA of apoE(-/-) mice was 1.9 and 2.5 times higher than the background, respectively. We conclude that the ORO solubilisation technique applied to AA samples is a very useful and rapid method for atherosclerotic lesion quantification. Copyright (C) 2009 S. Karger AG, Basel
| Original language | English |
|---|---|
| Pages (from-to) | 347-352 |
| Number of pages | 6 |
| Journal | Journal of Vascular Research |
| Volume | 46 |
| Issue number | 4 |
| Early online date | 10 Jan 2009 |
| DOIs | |
| Publication status | Published - Jun 2009 |
Keywords
- atherosclerosis
- atherosclerotic lesions
- apoE(-/-) mice
- plaque quantification
- lysochrome
- oil red o
- mouse models
- apolipoprotein-E
- deficient mice
- apoe
- apoE–/– mice
Cite this
Rapid Quantification of Aortic Lesions in ApoE(-/-) Mice. / Beattie, John H; Duthie, Susan J; Kwun, In-Sook; Ha, Tae-Youl; Gordon, Margaret J.
In: Journal of Vascular Research, Vol. 46, No. 4, 06.2009, p. 347-352.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid Quantification of Aortic Lesions in ApoE(-/-) Mice
AU - Beattie, John H
AU - Duthie, Susan J
AU - Kwun, In-Sook
AU - Ha, Tae-Youl
AU - Gordon, Margaret J
PY - 2009/6
Y1 - 2009/6
N2 - The quantification of aortic lesions is an important endpoint analysis for evaluating atherogenesis in mouse models of atherosclerosis. Morphometric methods involving the staining of aorta with a Sudan lysochrome followed by image analysis of the stained lesion area are commonly used. We have developed a more rapid method involving solubilisation of the stain retained by aortic lesions. In 2 separate studies, 5-week-old male apoE(-/-) and C57BL/6 wild-type (apoE(+/+)) mice were given a high fat (21%), Western-type diet for 13, 15 or 25 weeks. At study termination, the descending thoracic aorta (DA) and/or aortic arch (AA) were stained with Oil Red O (ORO). The incorporated stain was extracted using chloroform/methanol (2:1) solvent and quantified by spectrophotometry at 520 nm. In study 1 (13 weeks), ORO stain in the AA and DA of apoE(-/-) mice was 1.9 and 1.4 times higher than background staining of apoE(+/+) aorta tissue, respectively. At 15 and 25 weeks (study 2), ORO stain in the AA of apoE(-/-) mice was 1.9 and 2.5 times higher than the background, respectively. We conclude that the ORO solubilisation technique applied to AA samples is a very useful and rapid method for atherosclerotic lesion quantification. Copyright (C) 2009 S. Karger AG, Basel
AB - The quantification of aortic lesions is an important endpoint analysis for evaluating atherogenesis in mouse models of atherosclerosis. Morphometric methods involving the staining of aorta with a Sudan lysochrome followed by image analysis of the stained lesion area are commonly used. We have developed a more rapid method involving solubilisation of the stain retained by aortic lesions. In 2 separate studies, 5-week-old male apoE(-/-) and C57BL/6 wild-type (apoE(+/+)) mice were given a high fat (21%), Western-type diet for 13, 15 or 25 weeks. At study termination, the descending thoracic aorta (DA) and/or aortic arch (AA) were stained with Oil Red O (ORO). The incorporated stain was extracted using chloroform/methanol (2:1) solvent and quantified by spectrophotometry at 520 nm. In study 1 (13 weeks), ORO stain in the AA and DA of apoE(-/-) mice was 1.9 and 1.4 times higher than background staining of apoE(+/+) aorta tissue, respectively. At 15 and 25 weeks (study 2), ORO stain in the AA of apoE(-/-) mice was 1.9 and 2.5 times higher than the background, respectively. We conclude that the ORO solubilisation technique applied to AA samples is a very useful and rapid method for atherosclerotic lesion quantification. Copyright (C) 2009 S. Karger AG, Basel
KW - atherosclerosis
KW - atherosclerotic lesions
KW - apoE(-/-) mice
KW - plaque quantification
KW - lysochrome
KW - oil red o
KW - mouse models
KW - apolipoprotein-E
KW - deficient mice
KW - apoe
KW - apoE–/– mice
U2 - 10.1159/000189795
DO - 10.1159/000189795
M3 - Article
VL - 46
SP - 347
EP - 352
JO - Journal of Vascular Research
JF - Journal of Vascular Research
SN - 1018-1172
IS - 4
ER -