Rapid Response of Protein Synthesis to Insulin in 3T3 cells: Effects of Protein Kinase C Depletion and Differences from the Response to Serum Repletion

J E HESKETH, Gillian Patricia Campbell, P J REEDS

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    Abstract

    Quiescent 3T3 cells grown in media containing 4 ~o foetal calf serum showed different
    responses to insulin and to serum repletion (to 12~). Insulin stimulated protein
    synthesis within 1 h and this early response was insensitive to actinomycin D. The later
    insulin response showed progressive sensitivity to actinomycin D. The serum response
    was slower, not occurring until 1 h, and was inhibited by actinomycin D. Depletion of
    cell protein kinase C by pre-treatment with phorbol ester caused a total block of the
    immediate response to insulin but had little effect on the response to serum or the later
    response to insulin. Acute phorbol ester treatment stimulated protein synthesis.
    Original languageEnglish
    Pages (from-to)797-804
    Number of pages8
    JournalBioscience Reports
    Volume6
    Issue number9
    DOIs
    Publication statusPublished - Sep 1986

    Keywords

    • insulin
    • serum
    • protein synthesis
    • protein kinase C
    • 3T3 fibroblasts
    • signal transduction

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