Re-examination of the rainbow trout (Oncorhynchus mykiss) immune response to flagellin: Yersinia ruckeri flagellin is a potent activator of acute phase proteins, anti-microbial peptides and pro-inflammatory cytokines in vitro

Eakapol Wangkahart; Callum Scott; Christopher J. Secombes; Tiehui Wang

doi:10.1016/j.dci.2015.12.017

Re-examination of the rainbow trout (Oncorhynchus mykiss) immune response to flagellin: Yersinia ruckeri flagellin is a potent activator of acute phase proteins, anti-microbial peptides and pro-inflammatory cytokines in vitro

Eakapol Wangkahart, Callum Scott, Christopher J. Secombes, Tiehui Wang

Mahasarakham University

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

18 Downloads (Pure)

Abstract

Flagellin is the principal component of bacterial flagellum and a major target of the host immune system. To provide new insights into the role of flagellin in fish immune responses to flagellated microorganisms, a recombinant flagellin from Y. ruckeri (rYRF) was produced and its bioactivity investigated in the trout macrophage cell line RTS-11 and head kidney cells. rYRF is a potent activator of pro-inflammatory cytokines, acute phase proteins, antimicrobial peptides and subunits of the IL-12 cytokine family. This and the synergy seen with IFN-γ to enhance further expression of specific IL-12 and TNF-α isoforms may suggest that flagellin could be a useful immune stimulant or adjuvant for use in aquaculture. Gene paralogues were often differentially modulated, highlighting the need to study all of the paralogues of immune genes in fish to gain a full understanding of the effects of PAMPs or other stimulants, and the potential immune responses elicited.

Original language	English
Pages (from-to)	75-87
Number of pages	13
Journal	Developmental and Comparative Immunology
Volume	57
Early online date	21 Dec 2015
DOIs	https://doi.org/10.1016/j.dci.2015.12.017
Publication status	Published - Apr 2016

Bibliographical note

Acknowledgements
E.W. was supported by a PhD studentship from the Ministry of Science and Technology of Thailand and Mahasarakham University. T.W. received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland), that is funded by the Scottish Funding Council (grant reference HR09011). This research was also funded by the European Commission under the 7th Framework Programme for Research and Technological Development (FP7) of the European Union (grant agreement No. 311993 TARGETFISH).

Keywords

flagellin
Yersinia ruckeri
rainbow trout
cytokine
acute phase protein
antimicrobial peptide

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Wangkahart, E., Scott, C., Secombes, C. J., & Wang, T. (2016). Re-examination of the rainbow trout (Oncorhynchus mykiss) immune response to flagellin: Yersinia ruckeri flagellin is a potent activator of acute phase proteins, anti-microbial peptides and pro-inflammatory cytokines in vitro. Developmental and Comparative Immunology, 57, 75-87. https://doi.org/10.1016/j.dci.2015.12.017

Re-examination of the rainbow trout (Oncorhynchus mykiss) immune response to flagellin: Yersinia ruckeri flagellin is a potent activator of acute phase proteins, anti-microbial peptides and pro-inflammatory cytokines in vitro. / Wangkahart, Eakapol; Scott, Callum; Secombes, Christopher J. et al.
In: Developmental and Comparative Immunology, Vol. 57, 04.2016, p. 75-87.

Research output: Contribution to journal › Article › peer-review

Wangkahart, E, Scott, C, Secombes, CJ & Wang, T 2016, 'Re-examination of the rainbow trout (Oncorhynchus mykiss) immune response to flagellin: Yersinia ruckeri flagellin is a potent activator of acute phase proteins, anti-microbial peptides and pro-inflammatory cytokines in vitro', Developmental and Comparative Immunology, vol. 57, pp. 75-87. https://doi.org/10.1016/j.dci.2015.12.017

Wangkahart E, Scott C, Secombes CJ, Wang T. Re-examination of the rainbow trout (Oncorhynchus mykiss) immune response to flagellin: Yersinia ruckeri flagellin is a potent activator of acute phase proteins, anti-microbial peptides and pro-inflammatory cytokines in vitro. Developmental and Comparative Immunology. 2016 Apr;57:75-87. Epub 2015 Dec 21. doi: 10.1016/j.dci.2015.12.017

Wangkahart, Eakapol ; Scott, Callum ; Secombes, Christopher J. et al. / Re-examination of the rainbow trout (Oncorhynchus mykiss) immune response to flagellin : Yersinia ruckeri flagellin is a potent activator of acute phase proteins, anti-microbial peptides and pro-inflammatory cytokines in vitro. In: Developmental and Comparative Immunology. 2016 ; Vol. 57. pp. 75-87.

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abstract = "Flagellin is the principal component of bacterial flagellum and a major target of the host immune system. To provide new insights into the role of flagellin in fish immune responses to flagellated microorganisms, a recombinant flagellin from Y. ruckeri (rYRF) was produced and its bioactivity investigated in the trout macrophage cell line RTS-11 and head kidney cells. rYRF is a potent activator of pro-inflammatory cytokines, acute phase proteins, antimicrobial peptides and subunits of the IL-12 cytokine family. This and the synergy seen with IFN-γ to enhance further expression of specific IL-12 and TNF-α isoforms may suggest that flagellin could be a useful immune stimulant or adjuvant for use in aquaculture. Gene paralogues were often differentially modulated, highlighting the need to study all of the paralogues of immune genes in fish to gain a full understanding of the effects of PAMPs or other stimulants, and the potential immune responses elicited.",

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