Recognition of bacterial capsular polysaccharides and lipopolysaccharides by the macrophage mannose receptor

S. Zamze, L. Martinez-Pomares, H. Jones, P. R. Taylor, R. J. Stillion, S. Gordon, Simon Yuk Chun Wong

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140 Citations (Scopus)

Abstract

The in vitro binding of the macrophage mannose receptor to a range of different bacterial polysaccharides was investigated. The receptor was shown to bind to purified capsular polysaccharides from Streptococcus pneumoniae and to the lipopolysaccharides, but not capsular polysaccharides, from Klebsiella pneumoniae. Binding was Ca2+-dependent and inhibitable with D-mannose. A fusion protein of the mannose receptor containing carbohydrate recognition domains 4-7 and a full-length soluble form of the mannose receptor containing all domains external to the transmembrane region both displayed very similar binding specificities toward bacterial polysaccharides, suggesting that domains 4-7 are sufficient for recognition of these structures. Surprisingly, no direct correlation could be made between polysaccharide structure and binding to the mannose receptor, suggesting that polysaccharide conformation may play an important role in recognition. The full-length soluble form of the mannose receptor was able to bind simultaneously both polysaccharide via the carbohydrate recognition domains and sulfated oli-gosaccharide via the cysteine-rich domain. The possible involvement of the mannose receptor, either cell surface or soluble, in the innate and adaptive immune responses to bacterial polysaccharides is discussed.

Original languageEnglish
Pages (from-to)41613-41623
Number of pages10
JournalThe Journal of Biological Chemistry
Volume277
Issue number44
DOIs
Publication statusPublished - Nov 2002

Keywords

  • CYSTEINE-RICH DOMAIN
  • KLEBSIELLA-PNEUMONIAE
  • BINDING LECTIN
  • DENDRITIC CELLS
  • CONFORMATIONAL-ANALYSIS
  • NEISSERIA-MENINGITIDIS
  • POLYACRYLAMIDE GELS
  • ENDOTHELIAL-CELLS
  • INNATE IMMUNITY
  • MARGINAL ZONE

Cite this

Zamze, S., Martinez-Pomares, L., Jones, H., Taylor, P. R., Stillion, R. J., Gordon, S., & Wong, S. Y. C. (2002). Recognition of bacterial capsular polysaccharides and lipopolysaccharides by the macrophage mannose receptor. The Journal of Biological Chemistry, 277(44), 41613-41623. https://doi.org/10.1074/jbc.M207057200