Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus

Mark H T Stappers, Alexandra E Clark, Vishukumar Aimanianda, Stefan Bidula, Delyth M Reid, Patawee Asamaphan, Sarah E Hardison, Ivy M Dambuza, Isabel Valsecchi, Bernhard Kerscher, Anthony Plato, Carol A Wallace, Raif Yuecel, Betty Hebecker, Maria da Glória Teixeira Sousa, Cristina Cunha, Yan Liu, Ten Feizi, Axel A Brakhage, Kyung J Kwon-Chung & 13 others Neil A R Gow, Matteo Zanda, Monica Piras, Chiara Zanato, Martin Jaeger, Mihai G Netea, Frank L van de Veerdonk, João F Lacerda, António Campos, Agostinho Carvalho, Janet A Willment, Jean-Paul Latgé, Gordon D Brown

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Abstract

Resistance to infection is critically dependent on the ability of pattern recognition receptors to recognize microbial invasion and induce protective immune responses. One such family of receptors are the C-type lectins, which are central to antifungal immunity. These receptors activate key effector mechanisms upon recognition of conserved fungal cell-wall carbohydrates. However, several other immunologically active fungal ligands have been described; these include melanin, for which the mechanism of recognition is hitherto undefined. Here we identify a C-type lectin receptor, melanin-sensing C-type lectin receptor (MelLec), that has an essential role in antifungal immunity through recognition of the naphthalene-diol unit of 1,8-dihydroxynaphthalene (DHN)-melanin. MelLec recognizes melanin in conidial spores of Aspergillus fumigatus as well as in other DHN-melanized fungi. MelLec is ubiquitously expressed by CD31+endothelial cells in mice, and is also expressed by a sub-population of these cells that co-express epithelial cell adhesion molecule and are detected only in the lung and the liver. In mouse models, MelLec was required for protection against disseminated infection with A. fumigatus. In humans, MelLec is also expressed by myeloid cells, and we identified a single nucleotide polymorphism of this receptor that negatively affected myeloid inflammatory responses and significantly increased the susceptibility of stem-cell transplant recipients to disseminated Aspergillus infections. MelLec therefore recognizes an immunologically active component commonly found on fungi and has an essential role in protective antifungal immunity in both mice and humans.

Original languageEnglish
Pages (from-to)382-386
Number of pages5
JournalNature
Volume555
Issue number7696
Early online date28 Feb 2018
DOIs
Publication statusPublished - 15 Mar 2018

Fingerprint

C-Type Lectins
Melanins
Aspergillus
Immunity
Aspergillus fumigatus
Fungi
Infection
Pattern Recognition Receptors
Myeloid Cells
Spores
Cell Wall
Single Nucleotide Polymorphism
Stem Cells
Endothelial Cells
Carbohydrates
Ligands
Transplants
Lung
Liver
Population

Keywords

  • fungal host response
  • pattern recognition receptors

Cite this

Stappers, M. H. T., Clark, A. E., Aimanianda, V., Bidula, S., Reid, D. M., Asamaphan, P., ... Brown, G. D. (2018). Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus. Nature, 555(7696), 382-386. https://doi.org/10.1038/nature25974

Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus. / Stappers, Mark H T; Clark, Alexandra E; Aimanianda, Vishukumar; Bidula, Stefan; Reid, Delyth M; Asamaphan, Patawee; Hardison, Sarah E; Dambuza, Ivy M; Valsecchi, Isabel; Kerscher, Bernhard; Plato, Anthony; Wallace, Carol A; Yuecel, Raif; Hebecker, Betty; da Glória Teixeira Sousa, Maria; Cunha, Cristina; Liu, Yan; Feizi, Ten; Brakhage, Axel A; Kwon-Chung, Kyung J; Gow, Neil A R; Zanda, Matteo; Piras, Monica; Zanato, Chiara; Jaeger, Martin; Netea, Mihai G; van de Veerdonk, Frank L; Lacerda, João F; Campos, António; Carvalho, Agostinho; Willment, Janet A; Latgé, Jean-Paul; Brown, Gordon D.

In: Nature, Vol. 555, No. 7696, 15.03.2018, p. 382-386.

Research output: Contribution to journalArticle

Stappers, MHT, Clark, AE, Aimanianda, V, Bidula, S, Reid, DM, Asamaphan, P, Hardison, SE, Dambuza, IM, Valsecchi, I, Kerscher, B, Plato, A, Wallace, CA, Yuecel, R, Hebecker, B, da Glória Teixeira Sousa, M, Cunha, C, Liu, Y, Feizi, T, Brakhage, AA, Kwon-Chung, KJ, Gow, NAR, Zanda, M, Piras, M, Zanato, C, Jaeger, M, Netea, MG, van de Veerdonk, FL, Lacerda, JF, Campos, A, Carvalho, A, Willment, JA, Latgé, J-P & Brown, GD 2018, 'Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus', Nature, vol. 555, no. 7696, pp. 382-386. https://doi.org/10.1038/nature25974
Stappers MHT, Clark AE, Aimanianda V, Bidula S, Reid DM, Asamaphan P et al. Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus. Nature. 2018 Mar 15;555(7696):382-386. https://doi.org/10.1038/nature25974
Stappers, Mark H T ; Clark, Alexandra E ; Aimanianda, Vishukumar ; Bidula, Stefan ; Reid, Delyth M ; Asamaphan, Patawee ; Hardison, Sarah E ; Dambuza, Ivy M ; Valsecchi, Isabel ; Kerscher, Bernhard ; Plato, Anthony ; Wallace, Carol A ; Yuecel, Raif ; Hebecker, Betty ; da Glória Teixeira Sousa, Maria ; Cunha, Cristina ; Liu, Yan ; Feizi, Ten ; Brakhage, Axel A ; Kwon-Chung, Kyung J ; Gow, Neil A R ; Zanda, Matteo ; Piras, Monica ; Zanato, Chiara ; Jaeger, Martin ; Netea, Mihai G ; van de Veerdonk, Frank L ; Lacerda, João F ; Campos, António ; Carvalho, Agostinho ; Willment, Janet A ; Latgé, Jean-Paul ; Brown, Gordon D. / Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus. In: Nature. 2018 ; Vol. 555, No. 7696. pp. 382-386.
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abstract = "Resistance to infection is critically dependent on the ability of pattern recognition receptors to recognize microbial invasion and induce protective immune responses. One such family of receptors are the C-type lectins, which are central to antifungal immunity. These receptors activate key effector mechanisms upon recognition of conserved fungal cell-wall carbohydrates. However, several other immunologically active fungal ligands have been described; these include melanin, for which the mechanism of recognition is hitherto undefined. Here we identify a C-type lectin receptor, melanin-sensing C-type lectin receptor (MelLec), that has an essential role in antifungal immunity through recognition of the naphthalene-diol unit of 1,8-dihydroxynaphthalene (DHN)-melanin. MelLec recognizes melanin in conidial spores of Aspergillus fumigatus as well as in other DHN-melanized fungi. MelLec is ubiquitously expressed by CD31+endothelial cells in mice, and is also expressed by a sub-population of these cells that co-express epithelial cell adhesion molecule and are detected only in the lung and the liver. In mouse models, MelLec was required for protection against disseminated infection with A. fumigatus. In humans, MelLec is also expressed by myeloid cells, and we identified a single nucleotide polymorphism of this receptor that negatively affected myeloid inflammatory responses and significantly increased the susceptibility of stem-cell transplant recipients to disseminated Aspergillus infections. MelLec therefore recognizes an immunologically active component commonly found on fungi and has an essential role in protective antifungal immunity in both mice and humans.",
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AU - Aimanianda, Vishukumar

AU - Bidula, Stefan

AU - Reid, Delyth M

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AU - Plato, Anthony

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AU - Zanda, Matteo

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AU - Zanato, Chiara

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AU - Latgé, Jean-Paul

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N1 - We thank the staff of the University of Aberdeen animal facility for their support and care for our animals, C. G. Park for providing recombinant langerin, and S. Filler and R. Cramer for advice. Funding was provided by the Wellcome Trust (102705, 097377, 093378, 099197, 108430, 101873), the Medical Research Council Centre for Medical Mycology and the University of Aberdeen (MR/N006364/1). K.J.K.-C is supported by the intramural program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health; V.A. by an ANR-DST COMASPIN grant (ANR-13-ISV3-0004); B.H. by German Science Foundation (www.dfg.de) grant no. HE 7565/1-1; J.-P.L., I.V. and V.A. by the ANR and FRM DEQ2015-331722; A.C. by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER) (NORTE-01-0145-FEDER-000013), and by the Fundação para a Ciência e Tecnologia (FCT) (IF/00735/2014 and SFRH/BPD/96176/2013).

PY - 2018/3/15

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N2 - Resistance to infection is critically dependent on the ability of pattern recognition receptors to recognize microbial invasion and induce protective immune responses. One such family of receptors are the C-type lectins, which are central to antifungal immunity. These receptors activate key effector mechanisms upon recognition of conserved fungal cell-wall carbohydrates. However, several other immunologically active fungal ligands have been described; these include melanin, for which the mechanism of recognition is hitherto undefined. Here we identify a C-type lectin receptor, melanin-sensing C-type lectin receptor (MelLec), that has an essential role in antifungal immunity through recognition of the naphthalene-diol unit of 1,8-dihydroxynaphthalene (DHN)-melanin. MelLec recognizes melanin in conidial spores of Aspergillus fumigatus as well as in other DHN-melanized fungi. MelLec is ubiquitously expressed by CD31+endothelial cells in mice, and is also expressed by a sub-population of these cells that co-express epithelial cell adhesion molecule and are detected only in the lung and the liver. In mouse models, MelLec was required for protection against disseminated infection with A. fumigatus. In humans, MelLec is also expressed by myeloid cells, and we identified a single nucleotide polymorphism of this receptor that negatively affected myeloid inflammatory responses and significantly increased the susceptibility of stem-cell transplant recipients to disseminated Aspergillus infections. MelLec therefore recognizes an immunologically active component commonly found on fungi and has an essential role in protective antifungal immunity in both mice and humans.

AB - Resistance to infection is critically dependent on the ability of pattern recognition receptors to recognize microbial invasion and induce protective immune responses. One such family of receptors are the C-type lectins, which are central to antifungal immunity. These receptors activate key effector mechanisms upon recognition of conserved fungal cell-wall carbohydrates. However, several other immunologically active fungal ligands have been described; these include melanin, for which the mechanism of recognition is hitherto undefined. Here we identify a C-type lectin receptor, melanin-sensing C-type lectin receptor (MelLec), that has an essential role in antifungal immunity through recognition of the naphthalene-diol unit of 1,8-dihydroxynaphthalene (DHN)-melanin. MelLec recognizes melanin in conidial spores of Aspergillus fumigatus as well as in other DHN-melanized fungi. MelLec is ubiquitously expressed by CD31+endothelial cells in mice, and is also expressed by a sub-population of these cells that co-express epithelial cell adhesion molecule and are detected only in the lung and the liver. In mouse models, MelLec was required for protection against disseminated infection with A. fumigatus. In humans, MelLec is also expressed by myeloid cells, and we identified a single nucleotide polymorphism of this receptor that negatively affected myeloid inflammatory responses and significantly increased the susceptibility of stem-cell transplant recipients to disseminated Aspergillus infections. MelLec therefore recognizes an immunologically active component commonly found on fungi and has an essential role in protective antifungal immunity in both mice and humans.

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