Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus

Mark H T Stappers; Alexandra E Clark; Vishukumar Aimanianda; Stefan Bidula; Delyth M Reid; Patawee Asamaphan; Sarah E Hardison; Ivy M Dambuza; Isabel Valsecchi; Bernhard Kerscher; Anthony Plato; Carol A Wallace; Raif Yuecel; Betty Hebecker; Maria da Glória Teixeira Sousa; Cristina Cunha; Yan Liu; Ten Feizi; Axel A Brakhage; Kyung J Kwon-Chung; Neil A R Gow; Matteo Zanda; Monica Piras; Chiara Zanato; Martin Jaeger; Mihai G Netea; Frank L van de Veerdonk; João F Lacerda; António Campos; Agostinho Carvalho; Janet A Willment; Jean-Paul Latgé; Gordon D Brown

doi:10.1038/nature25974

Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus

Mark H T Stappers, Alexandra E Clark, Vishukumar Aimanianda, Stefan Bidula, Delyth M Reid, Patawee Asamaphan, Sarah E Hardison, Ivy M Dambuza, Isabel Valsecchi, Bernhard Kerscher, Anthony Plato, Carol A Wallace, Raif Yuecel, Betty Hebecker, Maria da Glória Teixeira Sousa, Cristina Cunha, Yan Liu, Ten Feizi, Axel A Brakhage, Kyung J Kwon-ChungNeil A R Gow, Matteo Zanda, Monica Piras, Chiara Zanato, Martin Jaeger, Mihai G Netea, Frank L van de Veerdonk, João F Lacerda, António Campos, Agostinho Carvalho, Janet A Willment, Jean-Paul Latgé, Gordon D Brown

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Abstract

Resistance to infection is critically dependent on the ability of pattern recognition receptors to recognize microbial invasion and induce protective immune responses. One such family of receptors are the C-type lectins, which are central to antifungal immunity. These receptors activate key effector mechanisms upon recognition of conserved fungal cell-wall carbohydrates. However, several other immunologically active fungal ligands have been described; these include melanin, for which the mechanism of recognition is hitherto undefined. Here we identify a C-type lectin receptor, melanin-sensing C-type lectin receptor (MelLec), that has an essential role in antifungal immunity through recognition of the naphthalene-diol unit of 1,8-dihydroxynaphthalene (DHN)-melanin. MelLec recognizes melanin in conidial spores of Aspergillus fumigatus as well as in other DHN-melanized fungi. MelLec is ubiquitously expressed by CD31+endothelial cells in mice, and is also expressed by a sub-population of these cells that co-express epithelial cell adhesion molecule and are detected only in the lung and the liver. In mouse models, MelLec was required for protection against disseminated infection with A. fumigatus. In humans, MelLec is also expressed by myeloid cells, and we identified a single nucleotide polymorphism of this receptor that negatively affected myeloid inflammatory responses and significantly increased the susceptibility of stem-cell transplant recipients to disseminated Aspergillus infections. MelLec therefore recognizes an immunologically active component commonly found on fungi and has an essential role in protective antifungal immunity in both mice and humans.

Original language	English
Pages (from-to)	382-386
Number of pages	5
Journal	Nature
Volume	555
Issue number	7696
Early online date	28 Feb 2018
DOIs	https://doi.org/10.1038/nature25974
Publication status	Published - 15 Mar 2018

Bibliographical note

We thank the staff of the University of Aberdeen animal facility for their support and care for our animals, C. G. Park for providing recombinant langerin, and S. Filler and R. Cramer for advice. Funding was provided by the Wellcome Trust (102705, 097377, 093378, 099197, 108430, 101873), the Medical Research Council Centre for Medical Mycology and the University of Aberdeen (MR/N006364/1). K.J.K.-C is supported by the intramural program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health; V.A. by an ANR-DST COMASPIN grant (ANR-13-ISV3-0004); B.H. by German Science Foundation (www.dfg.de) grant no. HE 7565/1-1; J.-P.L., I.V. and V.A. by the ANR and FRM DEQ2015-331722; A.C. by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER) (NORTE-01-0145-FEDER-000013), and by the Fundação para a Ciência e Tecnologia (FCT) (IF/00735/2014 and SFRH/BPD/96176/2013).

Keywords

fungal host response
pattern recognition receptors

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Stappers, M. H. T., Clark, A. E., Aimanianda, V., Bidula, S., Reid, D. M., Asamaphan, P., Hardison, S. E., Dambuza, I. M., Valsecchi, I., Kerscher, B., Plato, A., Wallace, C. A., Yuecel, R., Hebecker, B., da Glória Teixeira Sousa, M., Cunha, C., Liu, Y., Feizi, T., Brakhage, A. A., ... Brown, G. D. (2018). Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus. Nature, 555(7696), 382-386. https://doi.org/10.1038/nature25974

Stappers, MHT, Clark, AE, Aimanianda, V, Bidula, S, Reid, DM, Asamaphan, P, Hardison, SE, Dambuza, IM, Valsecchi, I, Kerscher, B, Plato, A, Wallace, CA, Yuecel, R, Hebecker, B, da Glória Teixeira Sousa, M, Cunha, C, Liu, Y, Feizi, T, Brakhage, AA, Kwon-Chung, KJ, Gow, NAR, Zanda, M, Piras, M, Zanato, C, Jaeger, M, Netea, MG, van de Veerdonk, FL, Lacerda, JF, Campos, A, Carvalho, A, Willment, JA, Latgé, J-P & Brown, GD 2018, 'Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus', Nature, vol. 555, no. 7696, pp. 382-386. https://doi.org/10.1038/nature25974

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title = "Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus",

abstract = "Resistance to infection is critically dependent on the ability of pattern recognition receptors to recognize microbial invasion and induce protective immune responses. One such family of receptors are the C-type lectins, which are central to antifungal immunity. These receptors activate key effector mechanisms upon recognition of conserved fungal cell-wall carbohydrates. However, several other immunologically active fungal ligands have been described; these include melanin, for which the mechanism of recognition is hitherto undefined. Here we identify a C-type lectin receptor, melanin-sensing C-type lectin receptor (MelLec), that has an essential role in antifungal immunity through recognition of the naphthalene-diol unit of 1,8-dihydroxynaphthalene (DHN)-melanin. MelLec recognizes melanin in conidial spores of Aspergillus fumigatus as well as in other DHN-melanized fungi. MelLec is ubiquitously expressed by CD31+endothelial cells in mice, and is also expressed by a sub-population of these cells that co-express epithelial cell adhesion molecule and are detected only in the lung and the liver. In mouse models, MelLec was required for protection against disseminated infection with A. fumigatus. In humans, MelLec is also expressed by myeloid cells, and we identified a single nucleotide polymorphism of this receptor that negatively affected myeloid inflammatory responses and significantly increased the susceptibility of stem-cell transplant recipients to disseminated Aspergillus infections. MelLec therefore recognizes an immunologically active component commonly found on fungi and has an essential role in protective antifungal immunity in both mice and humans.",

keywords = "fungal host response, pattern recognition receptors",

author = "Stappers, {Mark H T} and Clark, {Alexandra E} and Vishukumar Aimanianda and Stefan Bidula and Reid, {Delyth M} and Patawee Asamaphan and Hardison, {Sarah E} and Dambuza, {Ivy M} and Isabel Valsecchi and Bernhard Kerscher and Anthony Plato and Wallace, {Carol A} and Raif Yuecel and Betty Hebecker and {da Gl{\'o}ria Teixeira Sousa}, Maria and Cristina Cunha and Yan Liu and Ten Feizi and Brakhage, {Axel A} and Kwon-Chung, {Kyung J} and Gow, {Neil A R} and Matteo Zanda and Monica Piras and Chiara Zanato and Martin Jaeger and Netea, {Mihai G} and {van de Veerdonk}, {Frank L} and Lacerda, {Jo{\~a}o F} and Ant{\'o}nio Campos and Agostinho Carvalho and Willment, {Janet A} and Jean-Paul Latg{\'e} and Brown, {Gordon D}",

note = "We thank the staff of the University of Aberdeen animal facility for their support and care for our animals, C. G. Park for providing recombinant langerin, and S. Filler and R. Cramer for advice. Funding was provided by the Wellcome Trust (102705, 097377, 093378, 099197, 108430, 101873), the Medical Research Council Centre for Medical Mycology and the University of Aberdeen (MR/N006364/1). K.J.K.-C is supported by the intramural program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health; V.A. by an ANR-DST COMASPIN grant (ANR-13-ISV3-0004); B.H. by German Science Foundation (www.dfg.de) grant no. HE 7565/1-1; J.-P.L., I.V. and V.A. by the ANR and FRM DEQ2015-331722; A.C. by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER) (NORTE-01-0145-FEDER-000013), and by the Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia (FCT) (IF/00735/2014 and SFRH/BPD/96176/2013).",

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day = "15",

doi = "10.1038/nature25974",

language = "English",

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T1 - Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus

AU - Stappers, Mark H T

AU - Clark, Alexandra E

AU - Aimanianda, Vishukumar

AU - Bidula, Stefan

AU - Reid, Delyth M

AU - Asamaphan, Patawee

AU - Hardison, Sarah E

AU - Dambuza, Ivy M

AU - Valsecchi, Isabel

AU - Kerscher, Bernhard

AU - Plato, Anthony

AU - Wallace, Carol A

AU - Yuecel, Raif

AU - Hebecker, Betty

AU - da Glória Teixeira Sousa, Maria

AU - Cunha, Cristina

AU - Liu, Yan

AU - Feizi, Ten

AU - Brakhage, Axel A

AU - Kwon-Chung, Kyung J

AU - Gow, Neil A R

AU - Zanda, Matteo

AU - Piras, Monica

AU - Zanato, Chiara

AU - Jaeger, Martin

AU - Netea, Mihai G

AU - van de Veerdonk, Frank L

AU - Lacerda, João F

AU - Campos, António

AU - Carvalho, Agostinho

AU - Willment, Janet A

AU - Latgé, Jean-Paul

AU - Brown, Gordon D

N1 - We thank the staff of the University of Aberdeen animal facility for their support and care for our animals, C. G. Park for providing recombinant langerin, and S. Filler and R. Cramer for advice. Funding was provided by the Wellcome Trust (102705, 097377, 093378, 099197, 108430, 101873), the Medical Research Council Centre for Medical Mycology and the University of Aberdeen (MR/N006364/1). K.J.K.-C is supported by the intramural program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health; V.A. by an ANR-DST COMASPIN grant (ANR-13-ISV3-0004); B.H. by German Science Foundation (www.dfg.de) grant no. HE 7565/1-1; J.-P.L., I.V. and V.A. by the ANR and FRM DEQ2015-331722; A.C. by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER) (NORTE-01-0145-FEDER-000013), and by the Fundação para a Ciência e Tecnologia (FCT) (IF/00735/2014 and SFRH/BPD/96176/2013).

PY - 2018/3/15

Y1 - 2018/3/15

N2 - Resistance to infection is critically dependent on the ability of pattern recognition receptors to recognize microbial invasion and induce protective immune responses. One such family of receptors are the C-type lectins, which are central to antifungal immunity. These receptors activate key effector mechanisms upon recognition of conserved fungal cell-wall carbohydrates. However, several other immunologically active fungal ligands have been described; these include melanin, for which the mechanism of recognition is hitherto undefined. Here we identify a C-type lectin receptor, melanin-sensing C-type lectin receptor (MelLec), that has an essential role in antifungal immunity through recognition of the naphthalene-diol unit of 1,8-dihydroxynaphthalene (DHN)-melanin. MelLec recognizes melanin in conidial spores of Aspergillus fumigatus as well as in other DHN-melanized fungi. MelLec is ubiquitously expressed by CD31+endothelial cells in mice, and is also expressed by a sub-population of these cells that co-express epithelial cell adhesion molecule and are detected only in the lung and the liver. In mouse models, MelLec was required for protection against disseminated infection with A. fumigatus. In humans, MelLec is also expressed by myeloid cells, and we identified a single nucleotide polymorphism of this receptor that negatively affected myeloid inflammatory responses and significantly increased the susceptibility of stem-cell transplant recipients to disseminated Aspergillus infections. MelLec therefore recognizes an immunologically active component commonly found on fungi and has an essential role in protective antifungal immunity in both mice and humans.

AB - Resistance to infection is critically dependent on the ability of pattern recognition receptors to recognize microbial invasion and induce protective immune responses. One such family of receptors are the C-type lectins, which are central to antifungal immunity. These receptors activate key effector mechanisms upon recognition of conserved fungal cell-wall carbohydrates. However, several other immunologically active fungal ligands have been described; these include melanin, for which the mechanism of recognition is hitherto undefined. Here we identify a C-type lectin receptor, melanin-sensing C-type lectin receptor (MelLec), that has an essential role in antifungal immunity through recognition of the naphthalene-diol unit of 1,8-dihydroxynaphthalene (DHN)-melanin. MelLec recognizes melanin in conidial spores of Aspergillus fumigatus as well as in other DHN-melanized fungi. MelLec is ubiquitously expressed by CD31+endothelial cells in mice, and is also expressed by a sub-population of these cells that co-express epithelial cell adhesion molecule and are detected only in the lung and the liver. In mouse models, MelLec was required for protection against disseminated infection with A. fumigatus. In humans, MelLec is also expressed by myeloid cells, and we identified a single nucleotide polymorphism of this receptor that negatively affected myeloid inflammatory responses and significantly increased the susceptibility of stem-cell transplant recipients to disseminated Aspergillus infections. MelLec therefore recognizes an immunologically active component commonly found on fungi and has an essential role in protective antifungal immunity in both mice and humans.

KW - fungal host response

KW - pattern recognition receptors

U2 - 10.1038/nature25974

DO - 10.1038/nature25974

M3 - Article

C2 - 29489751

SN - 0028-0836

VL - 555

SP - 382

EP - 386

JO - Nature

JF - Nature

IS - 7696

ER -

Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus

Abstract

Bibliographical note

Keywords

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