Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors

Andreas F Kolb, Christopher Knowles, Patrikas Pultinevicius, Jennifer A Harbottle, Linda Petrie, Claire Robinson, David A Sorrell

Research output: Contribution to journalArticle

Abstract

Site-specific recombinases are important tools for the modification of mammalian genomes. In conjunction with viral vectors, they can be utilized to mediate site-specific gene insertions in animals and in cell lines which are difficult to transfect. Here we describe a method for the generation and analysis of an adenovirus vector supporting a recombinase-mediated cassette exchange reaction and discuss the advantages and limitations of this approach.

Original languageEnglish
Pages (from-to)127-150
Number of pages24
JournalMethods in Molecular Biology
Volume1642
DOIs
Publication statusPublished - 17 Aug 2017

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Recombinases
Insertional Mutagenesis
Adenoviridae
Genome
Cell Line
Site-specific recombinase

Keywords

  • Gene expression
  • Site-specific recombinase
  • Genomic target
  • Genome engineering
  • TCID50

Cite this

Kolb, A. F., Knowles, C., Pultinevicius, P., Harbottle, J. A., Petrie, L., Robinson, C., & Sorrell, D. A. (2017). Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors. Methods in Molecular Biology, 1642, 127-150. https://doi.org/10.1007/978-1-4939-7169-5_9

Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors. / Kolb, Andreas F; Knowles, Christopher; Pultinevicius, Patrikas; Harbottle, Jennifer A; Petrie, Linda; Robinson, Claire; Sorrell, David A.

In: Methods in Molecular Biology, Vol. 1642, 17.08.2017, p. 127-150.

Research output: Contribution to journalArticle

Kolb, AF, Knowles, C, Pultinevicius, P, Harbottle, JA, Petrie, L, Robinson, C & Sorrell, DA 2017, 'Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors', Methods in Molecular Biology, vol. 1642, pp. 127-150. https://doi.org/10.1007/978-1-4939-7169-5_9
Kolb AF, Knowles C, Pultinevicius P, Harbottle JA, Petrie L, Robinson C et al. Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors. Methods in Molecular Biology. 2017 Aug 17;1642:127-150. https://doi.org/10.1007/978-1-4939-7169-5_9
Kolb, Andreas F ; Knowles, Christopher ; Pultinevicius, Patrikas ; Harbottle, Jennifer A ; Petrie, Linda ; Robinson, Claire ; Sorrell, David A. / Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors. In: Methods in Molecular Biology. 2017 ; Vol. 1642. pp. 127-150.
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