Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors

Andreas F Kolb, Christopher Knowles, Patrikas Pultinevicius, Jennifer A Harbottle, Linda Petrie, Claire Robinson, David A Sorrell

Research output: Contribution to journalArticle

Abstract

Site-specific recombinases are important tools for the modification of mammalian genomes. In conjunction with viral vectors, they can be utilized to mediate site-specific gene insertions in animals and in cell lines which are difficult to transfect. Here we describe a method for the generation and analysis of an adenovirus vector supporting a recombinase-mediated cassette exchange reaction and discuss the advantages and limitations of this approach.

Original languageEnglish
Pages (from-to)127-150
Number of pages24
JournalMethods in Molecular Biology
Volume1642
Early online date17 Aug 2017
DOIs
Publication statusPublished - 17 Aug 2017

Keywords

  • Gene expression
  • Site-specific recombinase
  • Genomic target
  • Genome engineering
  • TCID50

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    Kolb, A. F., Knowles, C., Pultinevicius, P., Harbottle, J. A., Petrie, L., Robinson, C., & Sorrell, D. A. (2017). Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors. Methods in Molecular Biology, 1642, 127-150. https://doi.org/10.1007/978-1-4939-7169-5_9