Abstract
Site-specific recombinases are important tools for the modification of mammalian genomes. In conjunction with viral vectors, they can be utilized to mediate site-specific gene insertions in animals and in cell lines which are difficult to transfect. Here we describe a method for the generation and analysis of an adenovirus vector supporting a recombinase-mediated cassette exchange reaction and discuss the advantages and limitations of this approach.
Original language | English |
---|---|
Pages (from-to) | 127-150 |
Number of pages | 24 |
Journal | Methods in Molecular Biology |
Volume | 1642 |
Early online date | 17 Aug 2017 |
DOIs | |
Publication status | Published - 17 Aug 2017 |
Keywords
- Gene expression
- Site-specific recombinase
- Genomic target
- Genome engineering
- TCID50