Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance

Huan Cao; Aristotelis  Antonopoulos; Sadie Henderson; Heather J.h Wassall; John  Brewin; Alanna  Masson; Jenna Shepherd; Gabriela  Konieczny; Bhinal  Patel; Maria-Louise Williams; Adam Davie; Megan Amy Forrester; Lindsay Susan Hall; Beverley Minter; Dimitris  Tampakis; Michael Moss; Charlotte Lennon; Wendy Pickford; Lars Erwig; Beverley  Robertson; Anne  Dell; Gordon Brown; Heather Wilson; David C.  Rees; Stuart M Haslam; J. Alexandra Rowe; Robert Barker; Mark Vickers

doi:10.1038/s41467-021-21814-z

Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance

Huan Cao, Aristotelis Antonopoulos, Sadie Henderson, Heather J.h Wassall, John Brewin, Alanna Masson, Jenna Shepherd, Gabriela Konieczny, Bhinal Patel, Maria-Louise Williams, Adam Davie, Megan Amy Forrester, Lindsay Susan Hall, Beverley Minter, Dimitris Tampakis, Michael Moss, Charlotte Lennon, Wendy Pickford, Lars Erwig, Beverley RobertsonAnne Dell, Gordon Brown, Heather Wilson, David C. Rees, Stuart M Haslam, J. Alexandra Rowe, Robert Barker, Mark Vickers^* (Corresponding Author)

^*Corresponding author for this work

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Abstract

In both sickle cell disease and malaria, red blood cells (RBCs) are phagocytosed in the spleen, but receptor-ligand pairs mediating uptake have not been identified. Here, we report that patches of high mannose N-glycans (Man5-9GlcNAc2), expressed on diseased or oxidized RBC surfaces, bind the mannose receptor (CD206) on phagocytes to mediate clearance. We find that extravascular hemolysis in sickle cell disease correlates with high mannose glycan levels on RBCs. Furthermore, Plasmodium falciparum-infected RBCs expose surface mannose N-glycans, which occur at significantly higher levels on infected RBCs from sickle cell trait subjects compared to those lacking hemoglobin S. The glycans are associated with high molecular weight complexes and protease-resistant, lower molecular weight fragments containing spectrin. Recognition of surface N-linked high mannose glycans as a response to cellular stress is a molecular mechanism common to both the pathogenesis of sickle cell disease and resistance to severe malaria in sickle cell trait

Original language	English
Article number	1792
Number of pages	13
Journal	Nature Communications
Volume	12
Issue number	1
Early online date	19 Mar 2021
DOIs	https://doi.org/10.1038/s41467-021-21814-z
Publication status	Published - 19 Mar 2021

Keywords

Glycobiology
Malaria
Pattern recognition receptors
sickle cell disease

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cao, H., Antonopoulos, A., Henderson, S., Wassall, H. J. H., Brewin, J., Masson, A., Shepherd, J., Konieczny, G., Patel, B., Williams, M-L., Davie, A., Forrester, M. A., Hall, L. S., Minter, B., Tampakis, D., Moss, M., Lennon, C., Pickford, W., Erwig, L., ... Vickers, M. (2021). Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance. Nature Communications, 12(1), [1792]. https://doi.org/10.1038/s41467-021-21814-z

Cao, H, Antonopoulos, A, Henderson, S, Wassall, HJH, Brewin, J, Masson, A, Shepherd, J, Konieczny, G, Patel, B, Williams, M-L, Davie, A, Forrester, MA, Hall, LS, Minter, B, Tampakis, D, Moss, M, Lennon, C, Pickford, W, Erwig, L, Robertson, B, Dell, A, Brown, G, Wilson, H, Rees, DC, Haslam, SM, Rowe, JA, Barker, R & Vickers, M 2021, 'Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance', Nature Communications, vol. 12, no. 1, 1792. https://doi.org/10.1038/s41467-021-21814-z

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title = "Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance",

abstract = "In both sickle cell disease and malaria, red blood cells (RBCs) are phagocytosed in the spleen, but receptor-ligand pairs mediating uptake have not been identified. Here, we report that patches of high mannose N-glycans (Man5-9GlcNAc2), expressed on diseased or oxidized RBC surfaces, bind the mannose receptor (CD206) on phagocytes to mediate clearance. We find that extravascular hemolysis in sickle cell disease correlates with high mannose glycan levels on RBCs. Furthermore, Plasmodium falciparum-infected RBCs expose surface mannose N-glycans, which occur at significantly higher levels on infected RBCs from sickle cell trait subjects compared to those lacking hemoglobin S. The glycans are associated with high molecular weight complexes and protease-resistant, lower molecular weight fragments containing spectrin. Recognition of surface N-linked high mannose glycans as a response to cellular stress is a molecular mechanism common to both the pathogenesis of sickle cell disease and resistance to severe malaria in sickle cell trait",

keywords = "Glycobiology, Malaria, Pattern recognition receptors, sickle cell disease",

author = "Huan Cao and Aristotelis Antonopoulos and Sadie Henderson and Wassall, {Heather J.h} and John Brewin and Alanna Masson and Jenna Shepherd and Gabriela Konieczny and Bhinal Patel and Maria-Louise Williams and Adam Davie and Forrester, {Megan Amy} and Hall, {Lindsay Susan} and Beverley Minter and Dimitris Tampakis and Michael Moss and Charlotte Lennon and Wendy Pickford and Lars Erwig and Beverley Robertson and Anne Dell and Gordon Brown and Heather Wilson and Rees, {David C.} and Haslam, {Stuart M} and Rowe, {J. Alexandra} and Robert Barker and Mark Vickers",

note = "Acknowledgements We are grateful for the assistance provided by both the Microscopy and Histology Core Facility, and the Iain Fraser Cytometry Centre, at the University of Aberdeen. We thank Ann Wheeler and Matt Pearson from Edinburgh Super-Resolution Imaging Consortium for technical support with 3D SIM microscopy. We also thank Janet A. Willment and Bernard Kerscher, supervised by G.D.B., for providing the Fc fusion proteins, Jeanette A. Wagener, supervised by Neil A.R.G. Gow, for providing high purity chitin, Jan Westland for obtaining blood samples and Paul Crocker for useful discussions. Principal funding for this project was provided by Wellcome Trust grant 094847 (R.N.B., L.P.E., M.A.V.). In addition, support was provided by Biotechnology and Biological Sciences Research Council grants BBF0083091 (A.D. and S.M.H.) and BBK0161641 (A.D. and S.M.H.), Wellcome Trust grant 082098 (A.D.), Wellcome Trust grants 97377, 102705 (G.D.B.), and funding for the MRC Centre for Medical Mycology at the University of Aberdeen MR/N006364/1 (G.D.B.).",

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T1 - Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance

AU - Cao, Huan

AU - Antonopoulos, Aristotelis

AU - Henderson, Sadie

AU - Wassall, Heather J.h

AU - Brewin, John

AU - Masson, Alanna

AU - Shepherd, Jenna

AU - Konieczny, Gabriela

AU - Patel, Bhinal

AU - Williams, Maria-Louise

AU - Davie, Adam

AU - Forrester, Megan Amy

AU - Hall, Lindsay Susan

AU - Minter, Beverley

AU - Tampakis, Dimitris

AU - Moss, Michael

AU - Lennon, Charlotte

AU - Pickford, Wendy

AU - Erwig, Lars

AU - Robertson, Beverley

AU - Dell, Anne

AU - Brown, Gordon

AU - Wilson, Heather

AU - Rees, David C.

AU - Haslam, Stuart M

AU - Rowe, J. Alexandra

AU - Barker, Robert

AU - Vickers, Mark

N1 - Acknowledgements We are grateful for the assistance provided by both the Microscopy and Histology Core Facility, and the Iain Fraser Cytometry Centre, at the University of Aberdeen. We thank Ann Wheeler and Matt Pearson from Edinburgh Super-Resolution Imaging Consortium for technical support with 3D SIM microscopy. We also thank Janet A. Willment and Bernard Kerscher, supervised by G.D.B., for providing the Fc fusion proteins, Jeanette A. Wagener, supervised by Neil A.R.G. Gow, for providing high purity chitin, Jan Westland for obtaining blood samples and Paul Crocker for useful discussions. Principal funding for this project was provided by Wellcome Trust grant 094847 (R.N.B., L.P.E., M.A.V.). In addition, support was provided by Biotechnology and Biological Sciences Research Council grants BBF0083091 (A.D. and S.M.H.) and BBK0161641 (A.D. and S.M.H.), Wellcome Trust grant 082098 (A.D.), Wellcome Trust grants 97377, 102705 (G.D.B.), and funding for the MRC Centre for Medical Mycology at the University of Aberdeen MR/N006364/1 (G.D.B.).

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N2 - In both sickle cell disease and malaria, red blood cells (RBCs) are phagocytosed in the spleen, but receptor-ligand pairs mediating uptake have not been identified. Here, we report that patches of high mannose N-glycans (Man5-9GlcNAc2), expressed on diseased or oxidized RBC surfaces, bind the mannose receptor (CD206) on phagocytes to mediate clearance. We find that extravascular hemolysis in sickle cell disease correlates with high mannose glycan levels on RBCs. Furthermore, Plasmodium falciparum-infected RBCs expose surface mannose N-glycans, which occur at significantly higher levels on infected RBCs from sickle cell trait subjects compared to those lacking hemoglobin S. The glycans are associated with high molecular weight complexes and protease-resistant, lower molecular weight fragments containing spectrin. Recognition of surface N-linked high mannose glycans as a response to cellular stress is a molecular mechanism common to both the pathogenesis of sickle cell disease and resistance to severe malaria in sickle cell trait

AB - In both sickle cell disease and malaria, red blood cells (RBCs) are phagocytosed in the spleen, but receptor-ligand pairs mediating uptake have not been identified. Here, we report that patches of high mannose N-glycans (Man5-9GlcNAc2), expressed on diseased or oxidized RBC surfaces, bind the mannose receptor (CD206) on phagocytes to mediate clearance. We find that extravascular hemolysis in sickle cell disease correlates with high mannose glycan levels on RBCs. Furthermore, Plasmodium falciparum-infected RBCs expose surface mannose N-glycans, which occur at significantly higher levels on infected RBCs from sickle cell trait subjects compared to those lacking hemoglobin S. The glycans are associated with high molecular weight complexes and protease-resistant, lower molecular weight fragments containing spectrin. Recognition of surface N-linked high mannose glycans as a response to cellular stress is a molecular mechanism common to both the pathogenesis of sickle cell disease and resistance to severe malaria in sickle cell trait

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Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance

Abstract

Keywords

UN SDGs

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Iain Fraser Cytometry Centre

Microscopy and Histology

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Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance

Abstract

Keywords

UN SDGs

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Equipment

Iain Fraser Cytometry Centre

Microscopy and Histology

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