Redox components and structure of the respiratory NADH : ubiquinone oxidoreductase (complex I)

T Friedrich, A Abelmann, B Brors, V Guenebaut, L Kintscher, K Leonard, Tim Rasmussen, D Scheide, A Schlitt, U Schulte, H Weiss

Research output: Contribution to journalArticle

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Abstract

The proton-pumping NADH:ubiquinone oxidoreductase is the first complex in the respiratory chains of many purple bacteria and of mitochondria of most eucaryotes. The bacterial complex consists of 14 different subunits. The mitochondrial complex contains at least 29 additional proteins that do not directly participate in electron transfer and proton translocation. We analysed electron micrographs of isolated and negatively stained complex I particles from Escherichia coli and Neurospora crassa and obtained three-dimensional models of both complexes at medium resolution. Both have the same L-shaped overall structure with a peripheral arm protruding into the aqueous phase and a membrane arm extending into the membrane. The two arms of the bacterial complex are only slightly shorter than those of the mitochondrial complex although the protein mass of the former is only half of that of the latter. The presence of a novel redox group in the membrane arm of the complex is discussed. This group has been detected in the N. crassa complex by means of UV-visible spectroscopy. After reduction with an excess of NADH and reoxidation by the lactate dehydrogenase reaction, a reduced-minus-oxidized difference spectrum was obtained that cannot be attributed to the known cofactors flavin mononucleotide (FMN) and the FeS clusters N1, N2, N3 and N4. Due to its positive midpoint potential the novel group is believed to transfer electrons from the FeS clusters to ubiquinone. Its role in proton translocation is discussed. (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)215-219
Number of pages5
JournalBiochimica et Biophysica Acta. Bioenergetics
Volume1365
Issue number1-2
DOIs
Publication statusPublished - 10 Jun 1998

Keywords

  • NADH dehydrogenase
  • complex I
  • NADH : ubiquinone oxidoreductase
  • electron microscopy
  • EPR spectroscopy
  • FeS-cluster
  • Escherichia coli
  • Neurospora crassa
  • PROTON-TRANSLOCATING NADH
  • ACYL-CARRIER PROTEIN
  • MITOCHONDRIAL NADH
  • ESCHERICHIA-COLI
  • NEUROSPORA-CRASSA
  • REDUCTASE
  • SUBUNITS
  • GENES
  • ubiquinone oxidoreductase
  • Neurospora crassa

Cite this

Friedrich, T., Abelmann, A., Brors, B., Guenebaut, V., Kintscher, L., Leonard, K., ... Weiss, H. (1998). Redox components and structure of the respiratory NADH : ubiquinone oxidoreductase (complex I). Biochimica et Biophysica Acta. Bioenergetics, 1365(1-2), 215-219. https://doi.org/10.1016/S0005-2728(98)00070-X |

Redox components and structure of the respiratory NADH : ubiquinone oxidoreductase (complex I). / Friedrich, T ; Abelmann, A ; Brors, B ; Guenebaut, V ; Kintscher, L ; Leonard, K ; Rasmussen, Tim; Scheide, D ; Schlitt, A ; Schulte, U ; Weiss, H .

In: Biochimica et Biophysica Acta. Bioenergetics, Vol. 1365, No. 1-2, 10.06.1998, p. 215-219.

Research output: Contribution to journalArticle

Friedrich, T, Abelmann, A, Brors, B, Guenebaut, V, Kintscher, L, Leonard, K, Rasmussen, T, Scheide, D, Schlitt, A, Schulte, U & Weiss, H 1998, 'Redox components and structure of the respiratory NADH : ubiquinone oxidoreductase (complex I)', Biochimica et Biophysica Acta. Bioenergetics, vol. 1365, no. 1-2, pp. 215-219. https://doi.org/10.1016/S0005-2728(98)00070-X |
Friedrich, T ; Abelmann, A ; Brors, B ; Guenebaut, V ; Kintscher, L ; Leonard, K ; Rasmussen, Tim ; Scheide, D ; Schlitt, A ; Schulte, U ; Weiss, H . / Redox components and structure of the respiratory NADH : ubiquinone oxidoreductase (complex I). In: Biochimica et Biophysica Acta. Bioenergetics. 1998 ; Vol. 1365, No. 1-2. pp. 215-219.
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T2 - ubiquinone oxidoreductase (complex I)

AU - Friedrich, T

AU - Abelmann, A

AU - Brors, B

AU - Guenebaut, V

AU - Kintscher, L

AU - Leonard, K

AU - Rasmussen, Tim

AU - Scheide, D

AU - Schlitt, A

AU - Schulte, U

AU - Weiss, H

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N2 - The proton-pumping NADH:ubiquinone oxidoreductase is the first complex in the respiratory chains of many purple bacteria and of mitochondria of most eucaryotes. The bacterial complex consists of 14 different subunits. The mitochondrial complex contains at least 29 additional proteins that do not directly participate in electron transfer and proton translocation. We analysed electron micrographs of isolated and negatively stained complex I particles from Escherichia coli and Neurospora crassa and obtained three-dimensional models of both complexes at medium resolution. Both have the same L-shaped overall structure with a peripheral arm protruding into the aqueous phase and a membrane arm extending into the membrane. The two arms of the bacterial complex are only slightly shorter than those of the mitochondrial complex although the protein mass of the former is only half of that of the latter. The presence of a novel redox group in the membrane arm of the complex is discussed. This group has been detected in the N. crassa complex by means of UV-visible spectroscopy. After reduction with an excess of NADH and reoxidation by the lactate dehydrogenase reaction, a reduced-minus-oxidized difference spectrum was obtained that cannot be attributed to the known cofactors flavin mononucleotide (FMN) and the FeS clusters N1, N2, N3 and N4. Due to its positive midpoint potential the novel group is believed to transfer electrons from the FeS clusters to ubiquinone. Its role in proton translocation is discussed. (C) 1998 Elsevier Science B.V.

AB - The proton-pumping NADH:ubiquinone oxidoreductase is the first complex in the respiratory chains of many purple bacteria and of mitochondria of most eucaryotes. The bacterial complex consists of 14 different subunits. The mitochondrial complex contains at least 29 additional proteins that do not directly participate in electron transfer and proton translocation. We analysed electron micrographs of isolated and negatively stained complex I particles from Escherichia coli and Neurospora crassa and obtained three-dimensional models of both complexes at medium resolution. Both have the same L-shaped overall structure with a peripheral arm protruding into the aqueous phase and a membrane arm extending into the membrane. The two arms of the bacterial complex are only slightly shorter than those of the mitochondrial complex although the protein mass of the former is only half of that of the latter. The presence of a novel redox group in the membrane arm of the complex is discussed. This group has been detected in the N. crassa complex by means of UV-visible spectroscopy. After reduction with an excess of NADH and reoxidation by the lactate dehydrogenase reaction, a reduced-minus-oxidized difference spectrum was obtained that cannot be attributed to the known cofactors flavin mononucleotide (FMN) and the FeS clusters N1, N2, N3 and N4. Due to its positive midpoint potential the novel group is believed to transfer electrons from the FeS clusters to ubiquinone. Its role in proton translocation is discussed. (C) 1998 Elsevier Science B.V.

KW - NADH dehydrogenase

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KW - NADH : ubiquinone oxidoreductase

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KW - EPR spectroscopy

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KW - Neurospora crassa

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KW - ACYL-CARRIER PROTEIN

KW - MITOCHONDRIAL NADH

KW - ESCHERICHIA-COLI

KW - NEUROSPORA-CRASSA

KW - REDUCTASE

KW - SUBUNITS

KW - GENES

KW - ubiquinone oxidoreductase

KW - Neurospora crassa

U2 - 10.1016/S0005-2728(98)00070-X |

DO - 10.1016/S0005-2728(98)00070-X |

M3 - Article

VL - 1365

SP - 215

EP - 219

JO - Biochimica et Biophysica Acta. Bioenergetics

JF - Biochimica et Biophysica Acta. Bioenergetics

SN - 0005-2728

IS - 1-2

ER -