Reduced interleukin-18 levels in BAL specimens from patients with asthma compared to patients with sarcoidosis and healthy control subjects

L P Ho, M Davis, Alan Richard Denison, F T Wood , A P Greening

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    40 Citations (Scopus)

    Abstract

    Study objectives: To investigate whether differing airway interleukin (IL)-18 levels may be implicated in the pathogenesis of asthma and sarcoidosis.

    Setting: University teaching hospital.

    Patients and methods: IL-18 levels were measured in BAL fluid and in the supernatant of lipopolysaccharide (LPS)-stimulated alveolar macrophages obtained by BAL from 15 patients with sarcoidosis, 11 patients with asthma, and 13 healthy subjects. We also examined the relationship between IL-18 levels and macrophage and lymphocyte concentrations in BAL fluid. IL-18 was measured using an in-house enzyme-linked immunosorbent assay.

    Results: IL-18 levels were significantly lower in BAL fluid from patients with asthma (median, 0.0 pg/mL; interquartile range, 0.0 to 0.0 pg/mL) compared to patients with sarcoidosis (median, 222.0 pg/106; interquartile range, 110 to 340 pg/mL; p = 0.009, Mann Whitney rank-sum test) and healthy control subjects (median, 162 pg/mL; interquartile range, 38 to 203 pg/mL; p = 0.025, Mann Whitney rank-sum test). Individual analyses comparing IL-18 levels with BAL macrophage counts, and IL-18 with lymphocyte counts in the three groups showed no correlation between these indexes. The mean levels of IL-18 in unstimulated macrophage supernatants were 410 pg/106 cells for patients with asthma, 723.4 pg/106 cells for patients with sarcoidosis, and 734.8 pg/106 cells for healthy control subjects (p > 0.05). Stimulated macrophages from patients with sarcoidosis responded with increasing amounts of IL-18 at lower doses of LPS than macrophages from healthy control subjects or patients with asthma.

    Original languageEnglish
    Pages (from-to)1421-1426
    Number of pages6
    JournalChest
    Volume121
    Issue number5
    DOIs
    Publication statusPublished - May 2002

    Keywords

    • asthma
    • BAL
    • interleukin-18
    • macrophage stimulation
    • sarcoidosis
    • T-helper type 1/T-helper type 2 lymphocytes

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