Reduction in shear stress, activation of the endothelium, and leukocyte priming are all required for leukocyte passage across the blood--retina barrier

Heping Xu, Ayyakkannu Manivannan, Keith A Goatman, Hui-Rong Jiang, Janet Mary Liversidge, Peter Frederick Sharp, John Vincent Forrester, Isabel Joan Crane

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The passage of leukocytes across the blood-retina barrier at the early stages of an inflammatory reaction is influenced by a complex series of interactions about which little is known. In particular, the relationship between hydrodynamic factors, such as shear stress and leukocyte velocity, to the adherence and subsequent extravasation of leukocytes into the retina is unclear. We have used a physiological method, scanning laser ophthalmoscopy, to track labeled leukocytes circulating in the retina, followed by confocal microscopy of retinal flatmonnts to detect infiltrating cells at the early stage of experimental autoimmune uveitis. This has shown that retinal vessels are subjected to high shear stress under normal circumstances. During the inflammatory reaction, shear stress in retinal veins is reduced 24 It before leukocyte infiltration. This reduction is negatively correlated with leukocyte rolling and sticking in veins and postcapillary venules, the sites of leukocyte extravasation. Activation of vascular endothelial cells is also a prerequisite for leukocyte rolling and infiltration. In addition, antigen priming of leukocytes is influential at the early stage of inflammation, anti this is seen clearly in the reduction in rolling velocity and adherence of the printed leukocytes in activated retinal venules, 9 days postimmunization.

Original languageEnglish
Pages (from-to)224-232
Number of pages8
JournalJournal of Leukocyte Biology
Volume75
Issue number2
DOIs
Publication statusPublished - Feb 2004

Keywords

  • inflammation
  • leukocyte trafficking
  • blood flow
  • experimental autoimmune uveoretinitis
  • scanning laser ophthalmoscopy
  • EXPERIMENTAL AUTOIMMUNE UVEORETINITIS
  • IN-VIVO
  • MESENTERIC VENULES
  • ADHESION
  • EAU
  • MICE
  • TRAFFICKING
  • MIGRATION
  • MOLECULES
  • TRACKING

Cite this

Reduction in shear stress, activation of the endothelium, and leukocyte priming are all required for leukocyte passage across the blood--retina barrier. / Xu, Heping; Manivannan, Ayyakkannu; Goatman, Keith A; Jiang, Hui-Rong; Liversidge, Janet Mary; Sharp, Peter Frederick; Forrester, John Vincent; Crane, Isabel Joan.

In: Journal of Leukocyte Biology, Vol. 75, No. 2, 02.2004, p. 224-232.

Research output: Contribution to journalArticle

Xu, Heping ; Manivannan, Ayyakkannu ; Goatman, Keith A ; Jiang, Hui-Rong ; Liversidge, Janet Mary ; Sharp, Peter Frederick ; Forrester, John Vincent ; Crane, Isabel Joan. / Reduction in shear stress, activation of the endothelium, and leukocyte priming are all required for leukocyte passage across the blood--retina barrier. In: Journal of Leukocyte Biology. 2004 ; Vol. 75, No. 2. pp. 224-232.
@article{5cab8bca964844efa8f14d8d5054a79d,
title = "Reduction in shear stress, activation of the endothelium, and leukocyte priming are all required for leukocyte passage across the blood--retina barrier",
abstract = "The passage of leukocytes across the blood-retina barrier at the early stages of an inflammatory reaction is influenced by a complex series of interactions about which little is known. In particular, the relationship between hydrodynamic factors, such as shear stress and leukocyte velocity, to the adherence and subsequent extravasation of leukocytes into the retina is unclear. We have used a physiological method, scanning laser ophthalmoscopy, to track labeled leukocytes circulating in the retina, followed by confocal microscopy of retinal flatmonnts to detect infiltrating cells at the early stage of experimental autoimmune uveitis. This has shown that retinal vessels are subjected to high shear stress under normal circumstances. During the inflammatory reaction, shear stress in retinal veins is reduced 24 It before leukocyte infiltration. This reduction is negatively correlated with leukocyte rolling and sticking in veins and postcapillary venules, the sites of leukocyte extravasation. Activation of vascular endothelial cells is also a prerequisite for leukocyte rolling and infiltration. In addition, antigen priming of leukocytes is influential at the early stage of inflammation, anti this is seen clearly in the reduction in rolling velocity and adherence of the printed leukocytes in activated retinal venules, 9 days postimmunization.",
keywords = "inflammation, leukocyte trafficking, blood flow, experimental autoimmune uveoretinitis, scanning laser ophthalmoscopy, EXPERIMENTAL AUTOIMMUNE UVEORETINITIS, IN-VIVO, MESENTERIC VENULES, ADHESION, EAU, MICE, TRAFFICKING, MIGRATION, MOLECULES, TRACKING",
author = "Heping Xu and Ayyakkannu Manivannan and Goatman, {Keith A} and Hui-Rong Jiang and Liversidge, {Janet Mary} and Sharp, {Peter Frederick} and Forrester, {John Vincent} and Crane, {Isabel Joan}",
year = "2004",
month = "2",
doi = "10.1189/jlb.1002479",
language = "English",
volume = "75",
pages = "224--232",
journal = "Journal of Leukocyte Biology",
issn = "0741-5400",
publisher = "FASEB",
number = "2",

}

TY - JOUR

T1 - Reduction in shear stress, activation of the endothelium, and leukocyte priming are all required for leukocyte passage across the blood--retina barrier

AU - Xu, Heping

AU - Manivannan, Ayyakkannu

AU - Goatman, Keith A

AU - Jiang, Hui-Rong

AU - Liversidge, Janet Mary

AU - Sharp, Peter Frederick

AU - Forrester, John Vincent

AU - Crane, Isabel Joan

PY - 2004/2

Y1 - 2004/2

N2 - The passage of leukocytes across the blood-retina barrier at the early stages of an inflammatory reaction is influenced by a complex series of interactions about which little is known. In particular, the relationship between hydrodynamic factors, such as shear stress and leukocyte velocity, to the adherence and subsequent extravasation of leukocytes into the retina is unclear. We have used a physiological method, scanning laser ophthalmoscopy, to track labeled leukocytes circulating in the retina, followed by confocal microscopy of retinal flatmonnts to detect infiltrating cells at the early stage of experimental autoimmune uveitis. This has shown that retinal vessels are subjected to high shear stress under normal circumstances. During the inflammatory reaction, shear stress in retinal veins is reduced 24 It before leukocyte infiltration. This reduction is negatively correlated with leukocyte rolling and sticking in veins and postcapillary venules, the sites of leukocyte extravasation. Activation of vascular endothelial cells is also a prerequisite for leukocyte rolling and infiltration. In addition, antigen priming of leukocytes is influential at the early stage of inflammation, anti this is seen clearly in the reduction in rolling velocity and adherence of the printed leukocytes in activated retinal venules, 9 days postimmunization.

AB - The passage of leukocytes across the blood-retina barrier at the early stages of an inflammatory reaction is influenced by a complex series of interactions about which little is known. In particular, the relationship between hydrodynamic factors, such as shear stress and leukocyte velocity, to the adherence and subsequent extravasation of leukocytes into the retina is unclear. We have used a physiological method, scanning laser ophthalmoscopy, to track labeled leukocytes circulating in the retina, followed by confocal microscopy of retinal flatmonnts to detect infiltrating cells at the early stage of experimental autoimmune uveitis. This has shown that retinal vessels are subjected to high shear stress under normal circumstances. During the inflammatory reaction, shear stress in retinal veins is reduced 24 It before leukocyte infiltration. This reduction is negatively correlated with leukocyte rolling and sticking in veins and postcapillary venules, the sites of leukocyte extravasation. Activation of vascular endothelial cells is also a prerequisite for leukocyte rolling and infiltration. In addition, antigen priming of leukocytes is influential at the early stage of inflammation, anti this is seen clearly in the reduction in rolling velocity and adherence of the printed leukocytes in activated retinal venules, 9 days postimmunization.

KW - inflammation

KW - leukocyte trafficking

KW - blood flow

KW - experimental autoimmune uveoretinitis

KW - scanning laser ophthalmoscopy

KW - EXPERIMENTAL AUTOIMMUNE UVEORETINITIS

KW - IN-VIVO

KW - MESENTERIC VENULES

KW - ADHESION

KW - EAU

KW - MICE

KW - TRAFFICKING

KW - MIGRATION

KW - MOLECULES

KW - TRACKING

U2 - 10.1189/jlb.1002479

DO - 10.1189/jlb.1002479

M3 - Article

VL - 75

SP - 224

EP - 232

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

IS - 2

ER -