Reduction of Sensitization Induced By Blood Transfusion in the Rat By Monoclonal Antibodies

M C JONES, D J PROPPER, B K WEBER, Keith Nicol Stewart, G R D CATTO, C CUNNINGHAM

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Primary and secondary alloantibody responses were monitored in (AOxPVG)F1 hybrid rats after three transfusions of DA blood; the initial transfusion was either untreated or pretreated with monoclonal antibody directed to class I antigens or other cell surface markers. Mean antibody activity in recipient sera against class I DA antigens was significantly decreased by pretreatment with the monoclonal antibodies. The most marked suppression was associated with pretreatment by antibodies to the four major nonoverlapping epitopes of the RT1A(a) antigen. Subsequent transfusions of DA blood failed to stimulate a secondary response. Crossreactivity of the alloantibody reactivity with BDIX antigens was diminished by pretreating the transfusions with rat anti-RT1A antibodies and, to a lesser extent, with a mouse monoclonal antibody (OX-18) to a common class I determinant. Monoclonal antibody pretreatment had no effect on the humoral response to class II DA antigens. These studies indicate that blood transfusions pretreated with monoclonal antibodies induce a less-potent cytotoxic humoral immune response and that reactivity is most effectively suppressed by completely masking the class I antigen. This technique may prove of clinical value in preventing the sensitization caused by blood transfusions in potential transplant recipients.

Original languageEnglish
Pages (from-to)681-685
Number of pages5
JournalTransplantation
Volume51
Issue number3
Publication statusPublished - Mar 1991

Keywords

  • KIDNEY-GRAFT-SURVIVAL
  • RENAL-TRANSPLANTATION
  • SUPPRESSION
  • ANTIGENS
  • CELLS
  • CYCLOSPORINE
  • PREVENTION

Cite this

JONES, M. C., PROPPER, D. J., WEBER, B. K., Stewart, K. N., CATTO, G. R. D., & CUNNINGHAM, C. (1991). Reduction of Sensitization Induced By Blood Transfusion in the Rat By Monoclonal Antibodies. Transplantation, 51(3), 681-685.

Reduction of Sensitization Induced By Blood Transfusion in the Rat By Monoclonal Antibodies. / JONES, M C ; PROPPER, D J ; WEBER, B K ; Stewart, Keith Nicol; CATTO, G R D ; CUNNINGHAM, C .

In: Transplantation, Vol. 51, No. 3, 03.1991, p. 681-685.

Research output: Contribution to journalArticle

JONES, MC, PROPPER, DJ, WEBER, BK, Stewart, KN, CATTO, GRD & CUNNINGHAM, C 1991, 'Reduction of Sensitization Induced By Blood Transfusion in the Rat By Monoclonal Antibodies', Transplantation, vol. 51, no. 3, pp. 681-685.
JONES MC, PROPPER DJ, WEBER BK, Stewart KN, CATTO GRD, CUNNINGHAM C. Reduction of Sensitization Induced By Blood Transfusion in the Rat By Monoclonal Antibodies. Transplantation. 1991 Mar;51(3):681-685.
JONES, M C ; PROPPER, D J ; WEBER, B K ; Stewart, Keith Nicol ; CATTO, G R D ; CUNNINGHAM, C . / Reduction of Sensitization Induced By Blood Transfusion in the Rat By Monoclonal Antibodies. In: Transplantation. 1991 ; Vol. 51, No. 3. pp. 681-685.
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AB - Primary and secondary alloantibody responses were monitored in (AOxPVG)F1 hybrid rats after three transfusions of DA blood; the initial transfusion was either untreated or pretreated with monoclonal antibody directed to class I antigens or other cell surface markers. Mean antibody activity in recipient sera against class I DA antigens was significantly decreased by pretreatment with the monoclonal antibodies. The most marked suppression was associated with pretreatment by antibodies to the four major nonoverlapping epitopes of the RT1A(a) antigen. Subsequent transfusions of DA blood failed to stimulate a secondary response. Crossreactivity of the alloantibody reactivity with BDIX antigens was diminished by pretreating the transfusions with rat anti-RT1A antibodies and, to a lesser extent, with a mouse monoclonal antibody (OX-18) to a common class I determinant. Monoclonal antibody pretreatment had no effect on the humoral response to class II DA antigens. These studies indicate that blood transfusions pretreated with monoclonal antibodies induce a less-potent cytotoxic humoral immune response and that reactivity is most effectively suppressed by completely masking the class I antigen. This technique may prove of clinical value in preventing the sensitization caused by blood transfusions in potential transplant recipients.

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KW - CELLS

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