Regulation of adhesion molecule expression in human endothelial and smooth muscle cells by omega-3 fatty acids and conjugated linoleic acids: Involvement of the transcription factor NF-κB?

M Goua, S Mulgrew, J Frank, D Rees, A A Sneddon, K W J Wahle

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We previously showed conjugated linoleic acids (CLA) inhibited TNF-alpha-induced monocyte (THP-1) adhesion to human umbilical vein endothelial cells (HUVEC) in vitro which involved an increase in platelet activating factor (PAF). Here we show adhesion molecule (ADM) regulation by fatty acids and the differing role of nuclear factor kappa B (NF-kappa B) activation in HUVEC and vascular smooth muscle cells (vSMC). CLA and omega-3 long-chain polyunsaturated fatty acids (PUFA) (FA) reduced TNF-alpha-induced expression of ADMs (intercellular adhesion molecule-1 (ICAM-1); vascular cell adhesion molecule-1 (VCAM-1) but not E-selectin) on HUVEC and vSMC to different extents depending on FA type and concentration, cell type and method of analysis. I kappa B alpha phosphorylation in HUVEC and vSMC and transient transfection with NF-kappa B-luciferase reporter plasmid (HUVEC only) indicated differential NF-kappa B involvement during FA modulation (cis-9, trans-11; trans-10, cis-12 and a 50:50 mix of both CLA isomers; eicosapentaenoic acid (EPA); docosahexaenoic acid (DHA)). TNF-a-induced ADM expression in both cell types by 2-10-fold. In HUVEC, CLA t10, c12 and CLA mix (50:50 mixture of CLA c9, t11 and t10, c12) and EPA and DHA reduced ICAM-I expression (15-35%) at 12.5, 25 and/or 50 mu M. VCAM-I expression was reduced by 25 mu M 00, c12 isomer and mix; omega-3 PUFA and other concentrations of CLA and TNF-a-induced E-selectin expression were unaffected. TNF alpha-induced inhibitor kappa B (I kappa B) phosphorylation was biphasic peaking at 5 min in both cell types and 60 and 120 min in HUVEC and SMC, respectively. I kappa B alpha phosphorylation and NF-kappa B activity was reduced (29% and 30%, respectively) by 25 mu M CLA mix. n-3 PUFA did not reduce I kappa B alpha phosphorylation or NF-kappa B activity but reduced ADM expression. We show that n-3 PUFA and CLA reduce expression of ADM on HUVEC and vSMC. This reflected reduced adherence of monocytes to HUVEC previously reported by our group. Reduction of ICAM-1 and VCAM-1 protein expression by n-3 PUFA was less dependent on the NF-kappa B pathway than reduction by CLA which reflected the parallel attenuation of NF-kappa B activity. This indicated involvement of other transcription factors (i.e. AP-1) in the FA regulation of ADM expression and has, to our knowledge, not been previously reported. (C) 2007 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)33-43
Number of pages11
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume78
Issue number1
Early online date26 Nov 2007
DOIs
Publication statusPublished - Jan 2008

Keywords

  • Cell Line
  • Cells, Cultured
  • Endothelial Cells
  • Endothelium, Vascular
  • Fatty Acids, Omega-3
  • Humans
  • Intercellular Adhesion Molecule-1
  • Linoleic Acids, Conjugated
  • Muscle, Smooth, Vascular
  • Myocytes, Smooth Muscle
  • NF-kappa B
  • Phosphorylation
  • Tumor Necrosis Factor-alpha
  • Umbilical Veins
  • Vascular Cell Adhesion Molecule-1
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

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title = "Regulation of adhesion molecule expression in human endothelial and smooth muscle cells by omega-3 fatty acids and conjugated linoleic acids: Involvement of the transcription factor NF-κB?",
abstract = "We previously showed conjugated linoleic acids (CLA) inhibited TNF-alpha-induced monocyte (THP-1) adhesion to human umbilical vein endothelial cells (HUVEC) in vitro which involved an increase in platelet activating factor (PAF). Here we show adhesion molecule (ADM) regulation by fatty acids and the differing role of nuclear factor kappa B (NF-kappa B) activation in HUVEC and vascular smooth muscle cells (vSMC). CLA and omega-3 long-chain polyunsaturated fatty acids (PUFA) (FA) reduced TNF-alpha-induced expression of ADMs (intercellular adhesion molecule-1 (ICAM-1); vascular cell adhesion molecule-1 (VCAM-1) but not E-selectin) on HUVEC and vSMC to different extents depending on FA type and concentration, cell type and method of analysis. I kappa B alpha phosphorylation in HUVEC and vSMC and transient transfection with NF-kappa B-luciferase reporter plasmid (HUVEC only) indicated differential NF-kappa B involvement during FA modulation (cis-9, trans-11; trans-10, cis-12 and a 50:50 mix of both CLA isomers; eicosapentaenoic acid (EPA); docosahexaenoic acid (DHA)). TNF-a-induced ADM expression in both cell types by 2-10-fold. In HUVEC, CLA t10, c12 and CLA mix (50:50 mixture of CLA c9, t11 and t10, c12) and EPA and DHA reduced ICAM-I expression (15-35{\%}) at 12.5, 25 and/or 50 mu M. VCAM-I expression was reduced by 25 mu M 00, c12 isomer and mix; omega-3 PUFA and other concentrations of CLA and TNF-a-induced E-selectin expression were unaffected. TNF alpha-induced inhibitor kappa B (I kappa B) phosphorylation was biphasic peaking at 5 min in both cell types and 60 and 120 min in HUVEC and SMC, respectively. I kappa B alpha phosphorylation and NF-kappa B activity was reduced (29{\%} and 30{\%}, respectively) by 25 mu M CLA mix. n-3 PUFA did not reduce I kappa B alpha phosphorylation or NF-kappa B activity but reduced ADM expression. We show that n-3 PUFA and CLA reduce expression of ADM on HUVEC and vSMC. This reflected reduced adherence of monocytes to HUVEC previously reported by our group. Reduction of ICAM-1 and VCAM-1 protein expression by n-3 PUFA was less dependent on the NF-kappa B pathway than reduction by CLA which reflected the parallel attenuation of NF-kappa B activity. This indicated involvement of other transcription factors (i.e. AP-1) in the FA regulation of ADM expression and has, to our knowledge, not been previously reported. (C) 2007 Elsevier Ltd. All rights reserved.",
keywords = "Cell Line, Cells, Cultured, Endothelial Cells, Endothelium, Vascular, Fatty Acids, Omega-3, Humans, Intercellular Adhesion Molecule-1, Linoleic Acids, Conjugated, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, NF-kappa B, Phosphorylation, Tumor Necrosis Factor-alpha, Umbilical Veins, Vascular Cell Adhesion Molecule-1, Journal Article, Research Support, Non-U.S. Gov't",
author = "M Goua and S Mulgrew and J Frank and D Rees and Sneddon, {A A} and Wahle, {K W J}",
year = "2008",
month = "1",
doi = "10.1016/j.plefa.2007.10.004",
language = "English",
volume = "78",
pages = "33--43",
journal = "Prostaglandins, Leukotrienes and Essential Fatty Acids",
issn = "0952-3278",
publisher = "Churchill Livingstone",
number = "1",

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TY - JOUR

T1 - Regulation of adhesion molecule expression in human endothelial and smooth muscle cells by omega-3 fatty acids and conjugated linoleic acids

T2 - Involvement of the transcription factor NF-κB?

AU - Goua, M

AU - Mulgrew, S

AU - Frank, J

AU - Rees, D

AU - Sneddon, A A

AU - Wahle, K W J

PY - 2008/1

Y1 - 2008/1

N2 - We previously showed conjugated linoleic acids (CLA) inhibited TNF-alpha-induced monocyte (THP-1) adhesion to human umbilical vein endothelial cells (HUVEC) in vitro which involved an increase in platelet activating factor (PAF). Here we show adhesion molecule (ADM) regulation by fatty acids and the differing role of nuclear factor kappa B (NF-kappa B) activation in HUVEC and vascular smooth muscle cells (vSMC). CLA and omega-3 long-chain polyunsaturated fatty acids (PUFA) (FA) reduced TNF-alpha-induced expression of ADMs (intercellular adhesion molecule-1 (ICAM-1); vascular cell adhesion molecule-1 (VCAM-1) but not E-selectin) on HUVEC and vSMC to different extents depending on FA type and concentration, cell type and method of analysis. I kappa B alpha phosphorylation in HUVEC and vSMC and transient transfection with NF-kappa B-luciferase reporter plasmid (HUVEC only) indicated differential NF-kappa B involvement during FA modulation (cis-9, trans-11; trans-10, cis-12 and a 50:50 mix of both CLA isomers; eicosapentaenoic acid (EPA); docosahexaenoic acid (DHA)). TNF-a-induced ADM expression in both cell types by 2-10-fold. In HUVEC, CLA t10, c12 and CLA mix (50:50 mixture of CLA c9, t11 and t10, c12) and EPA and DHA reduced ICAM-I expression (15-35%) at 12.5, 25 and/or 50 mu M. VCAM-I expression was reduced by 25 mu M 00, c12 isomer and mix; omega-3 PUFA and other concentrations of CLA and TNF-a-induced E-selectin expression were unaffected. TNF alpha-induced inhibitor kappa B (I kappa B) phosphorylation was biphasic peaking at 5 min in both cell types and 60 and 120 min in HUVEC and SMC, respectively. I kappa B alpha phosphorylation and NF-kappa B activity was reduced (29% and 30%, respectively) by 25 mu M CLA mix. n-3 PUFA did not reduce I kappa B alpha phosphorylation or NF-kappa B activity but reduced ADM expression. We show that n-3 PUFA and CLA reduce expression of ADM on HUVEC and vSMC. This reflected reduced adherence of monocytes to HUVEC previously reported by our group. Reduction of ICAM-1 and VCAM-1 protein expression by n-3 PUFA was less dependent on the NF-kappa B pathway than reduction by CLA which reflected the parallel attenuation of NF-kappa B activity. This indicated involvement of other transcription factors (i.e. AP-1) in the FA regulation of ADM expression and has, to our knowledge, not been previously reported. (C) 2007 Elsevier Ltd. All rights reserved.

AB - We previously showed conjugated linoleic acids (CLA) inhibited TNF-alpha-induced monocyte (THP-1) adhesion to human umbilical vein endothelial cells (HUVEC) in vitro which involved an increase in platelet activating factor (PAF). Here we show adhesion molecule (ADM) regulation by fatty acids and the differing role of nuclear factor kappa B (NF-kappa B) activation in HUVEC and vascular smooth muscle cells (vSMC). CLA and omega-3 long-chain polyunsaturated fatty acids (PUFA) (FA) reduced TNF-alpha-induced expression of ADMs (intercellular adhesion molecule-1 (ICAM-1); vascular cell adhesion molecule-1 (VCAM-1) but not E-selectin) on HUVEC and vSMC to different extents depending on FA type and concentration, cell type and method of analysis. I kappa B alpha phosphorylation in HUVEC and vSMC and transient transfection with NF-kappa B-luciferase reporter plasmid (HUVEC only) indicated differential NF-kappa B involvement during FA modulation (cis-9, trans-11; trans-10, cis-12 and a 50:50 mix of both CLA isomers; eicosapentaenoic acid (EPA); docosahexaenoic acid (DHA)). TNF-a-induced ADM expression in both cell types by 2-10-fold. In HUVEC, CLA t10, c12 and CLA mix (50:50 mixture of CLA c9, t11 and t10, c12) and EPA and DHA reduced ICAM-I expression (15-35%) at 12.5, 25 and/or 50 mu M. VCAM-I expression was reduced by 25 mu M 00, c12 isomer and mix; omega-3 PUFA and other concentrations of CLA and TNF-a-induced E-selectin expression were unaffected. TNF alpha-induced inhibitor kappa B (I kappa B) phosphorylation was biphasic peaking at 5 min in both cell types and 60 and 120 min in HUVEC and SMC, respectively. I kappa B alpha phosphorylation and NF-kappa B activity was reduced (29% and 30%, respectively) by 25 mu M CLA mix. n-3 PUFA did not reduce I kappa B alpha phosphorylation or NF-kappa B activity but reduced ADM expression. We show that n-3 PUFA and CLA reduce expression of ADM on HUVEC and vSMC. This reflected reduced adherence of monocytes to HUVEC previously reported by our group. Reduction of ICAM-1 and VCAM-1 protein expression by n-3 PUFA was less dependent on the NF-kappa B pathway than reduction by CLA which reflected the parallel attenuation of NF-kappa B activity. This indicated involvement of other transcription factors (i.e. AP-1) in the FA regulation of ADM expression and has, to our knowledge, not been previously reported. (C) 2007 Elsevier Ltd. All rights reserved.

KW - Cell Line

KW - Cells, Cultured

KW - Endothelial Cells

KW - Endothelium, Vascular

KW - Fatty Acids, Omega-3

KW - Humans

KW - Intercellular Adhesion Molecule-1

KW - Linoleic Acids, Conjugated

KW - Muscle, Smooth, Vascular

KW - Myocytes, Smooth Muscle

KW - NF-kappa B

KW - Phosphorylation

KW - Tumor Necrosis Factor-alpha

KW - Umbilical Veins

KW - Vascular Cell Adhesion Molecule-1

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.plefa.2007.10.004

DO - 10.1016/j.plefa.2007.10.004

M3 - Article

C2 - 18036803

VL - 78

SP - 33

EP - 43

JO - Prostaglandins, Leukotrienes and Essential Fatty Acids

JF - Prostaglandins, Leukotrienes and Essential Fatty Acids

SN - 0952-3278

IS - 1

ER -