Regulation of constitutive exocytic transport by membrane receptors. A biochemical and morphometric study

R Buccione, S Bannykh, I Santone, M Baldassarre, F Facchiano, Y Bozzi, G Di Tullio, A Mironov, A Luini, M A De Matteis

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Abstract

Biochemical and morphometric approaches were combined to examine whether constitutive secretory transport might be controlled by plasma membrane receptors, as this possibility would have significant physiological implications. Indeed, IgE receptor stimulation in rat basophilic leukemia cells potently increased the rate of transport of soluble pulse-labeled 35S-sulfated glycosaminoglycans from distal Golgi compartments to the cell surface. This effect was largely protein kinase C (PKC)-dependent. Direct activation of PKC also stimulated constitutive transport of glycosaminoglycans, as indicated by the use of agonistic and antagonistic PKC ligands. PKC ligands also had potent, but different, effects on the exocytic transport from distal Golgi compartments to the plasma membrane of a membrane-bound protein (vesicular stomatitis virus glycoprotein), which was slightly stimulated by activators and profoundly suppressed by inhibitors of PKC. Morphological analysis showed impressive changes of the organelles of the secretory pathway in response to IgE receptor stimulation and to direct PKC activation (enhanced number of buds and vesicles originating from the endoplasmic reticulum and Golgi and increase in surface and volume of Golgi compartments), suggestive of an overall activation of exocytic movements. These results show that rapid and large changes in constitutive transport fluxes and in the morphology of the exocytic apparatus can be induced by membrane receptors (as well as by direct PKC stimulation).

Original languageEnglish
Pages (from-to)3523-33
Number of pages11
JournalThe Journal of Biological Chemistry
Volume271
Issue number7
Publication statusPublished - 16 Feb 1996

Keywords

  • Animals
  • Cell Line
  • Cell Membrane
  • Cell Nucleus
  • Cell-Free System
  • Cytoplasmic Granules
  • Dogs
  • Endoplasmic Reticulum
  • Enzyme Activation
  • Exocytosis
  • Glycosaminoglycans
  • Golgi Apparatus
  • HeLa Cells
  • Homeostasis
  • Humans
  • Kinetics
  • Leukemia, Basophilic, Acute
  • PC12 Cells
  • Protein Kinase C
  • Proteoglycans
  • Rats
  • Receptors, IgE
  • Sulfur Radioisotopes
  • Tetradecanoylphorbol Acetate
  • Tumor Cells, Cultured

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    Buccione, R., Bannykh, S., Santone, I., Baldassarre, M., Facchiano, F., Bozzi, Y., Di Tullio, G., Mironov, A., Luini, A., & De Matteis, M. A. (1996). Regulation of constitutive exocytic transport by membrane receptors. A biochemical and morphometric study. The Journal of Biological Chemistry, 271(7), 3523-33.