Regulation of GPR55 in rat white adipose tissue and serum LPI by nutritional status, gestation, gender and pituitary factors

Monica Imbernon, Lauren Whyte, Adenis Diaz-Arteaga, Wendy R. Russell, Natalia R. Moreno, María J. Vazquez, Carmen R. Gonzalez, Alberto Díaz-Ruiz, Miguel Lopez, Maria M. Malagón, Ruth A. Ross, Carlos Dieguez, Ruben Nogueiras

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21 Citations (Scopus)

Abstract

The G protein-coupled receptor GPR55 has been proposed as a new cannabinoid receptor associated with obesity in humans. We have investigated the regulation of GPR55 in rat white adipose tissue (WAT) in different physiological and pathophysiological settings involved in energy balance. We compared GPR55 expression with Cannabinoid Receptor type 1 (CB1), which mediates the metabolic actions of endocannabinoids, by real time PCR and western blotting. Circulating levels of lysophosphatidylinositol (LPI), the endogenous ligand of GPR55, were measured by liquid chromatography-mass spectrometry. Both WAT CB1 and GPR55 levels were increased after fasting and recovered after leptin treatment. Their expression was decreased during gestation and increased throughout lifespan. Orchidectomy diminished WAT CB1 and GPR55 expression whereas ovariectomized rats showed increased GPR55 but decreased CB1 levels. Alterations in pituitary functions also modified WAT CB1 and GPR55 levels. Serum LPI levels were inversely regulated by fasting and gonadectomy in comparison to WAT GPR55. Our findings indicate that GPR55 and LPI are regulated by different physiological and pathophysiological settings known to be associated with marked alterations in energy status.

Original languageEnglish
Pages (from-to)159-169
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume383
Issue number1-2
Early online date27 Dec 2013
DOIs
Publication statusPublished - 5 Mar 2014

Keywords

  • CB1
  • Endocannabinoids
  • GPR55
  • LPI
  • Nutritional status
  • White adipose tissue

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Biochemistry

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  • Cite this

    Imbernon, M., Whyte, L., Diaz-Arteaga, A., Russell, W. R., Moreno, N. R., Vazquez, M. J., Gonzalez, C. R., Díaz-Ruiz, A., Lopez, M., Malagón, M. M., Ross, R. A., Dieguez, C., & Nogueiras, R. (2014). Regulation of GPR55 in rat white adipose tissue and serum LPI by nutritional status, gestation, gender and pituitary factors. Molecular and Cellular Endocrinology, 383(1-2), 159-169. https://doi.org/10.1016/j.mce.2013.12.011