Regulation of interleukin 1 beta expression in the common carp, Cyprinus carpio L.

M. Engelsma, Rene Josephus Maria Stet, H. Schipper, B. M. L. Verburg-van Kemenade

    Research output: Contribution to journalArticle

    98 Citations (Scopus)

    Abstract

    The intron-exon organisation of the carp IL-1 beta gene consists of 2455 bp and comprises seven exons. Three IL-1 beta RNA transcripts have been found in carp: (1) a fully spliced product; (2) exon 1-7 with introns 5 and 6; and (3) exon 1-7 with intron 5 only. The intron-containing products probably represent partially spliced transcripts. IL-1 beta mRNA expression in carp was semi-quantitatively analysed by RT-PCR in multiple organs, including brain and pituitary. Constitutive expression of the IL-1 beta mRNA was found in these organs with a predominant expression in the immune organs head kidney and spleen. Furthermore, a scattered distribution of IL-1 beta producing cells was shown by in situ hybridisations of head kidney tissue. Administration of phorbol-myristate-acetate (PMA), lipopolysaccharide (LPS) or retinoic acid (RA), to phagocytes isolated from the head kidney, resulted in expression of IL-1 beta intron-containing transcripts. Of these, only PMA and LPS were stimulators that induced the fully spliced transcript. A role for the nuclear factor (NF)-kappaB pathway in carp IL-1 beta expression was shown with suppression of the LPS-induced IL-1 beta expression by NF-kappaB inhibitor pyrrolidine dithiocarbamate (PDTC). Cortisol was able to inhibit in vitro constitutive expression of IL-1 beta transcripts. Addition of cortisol simultaneously with LPS could not substantially inhibit transcription. (C) 2001 Elsevier Science Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)195-203
    Number of pages8
    JournalDevelopmental and Comparative Immunology
    Volume25
    DOIs
    Publication statusPublished - 2001

    Keywords

    • fish
    • Cyprinus carpio
    • interleukin 1
    • mRNA
    • gene expression
    • LPS
    • cortisol
    • NEUTROPHILIC GRANULOCYTES
    • GENE-EXPRESSION
    • MESSENGER-RNA
    • MOLECULAR-CLONING
    • RETINOIC ACID
    • MACROPHAGES
    • SEQUENCE
    • FISH
    • APOPTOSIS
    • SYSTEM

    Cite this

    Regulation of interleukin 1 beta expression in the common carp, Cyprinus carpio L. / Engelsma, M.; Stet, Rene Josephus Maria; Schipper, H.; Verburg-van Kemenade, B. M. L.

    In: Developmental and Comparative Immunology, Vol. 25, 2001, p. 195-203.

    Research output: Contribution to journalArticle

    Engelsma, M. ; Stet, Rene Josephus Maria ; Schipper, H. ; Verburg-van Kemenade, B. M. L. / Regulation of interleukin 1 beta expression in the common carp, Cyprinus carpio L. In: Developmental and Comparative Immunology. 2001 ; Vol. 25. pp. 195-203.
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    abstract = "The intron-exon organisation of the carp IL-1 beta gene consists of 2455 bp and comprises seven exons. Three IL-1 beta RNA transcripts have been found in carp: (1) a fully spliced product; (2) exon 1-7 with introns 5 and 6; and (3) exon 1-7 with intron 5 only. The intron-containing products probably represent partially spliced transcripts. IL-1 beta mRNA expression in carp was semi-quantitatively analysed by RT-PCR in multiple organs, including brain and pituitary. Constitutive expression of the IL-1 beta mRNA was found in these organs with a predominant expression in the immune organs head kidney and spleen. Furthermore, a scattered distribution of IL-1 beta producing cells was shown by in situ hybridisations of head kidney tissue. Administration of phorbol-myristate-acetate (PMA), lipopolysaccharide (LPS) or retinoic acid (RA), to phagocytes isolated from the head kidney, resulted in expression of IL-1 beta intron-containing transcripts. Of these, only PMA and LPS were stimulators that induced the fully spliced transcript. A role for the nuclear factor (NF)-kappaB pathway in carp IL-1 beta expression was shown with suppression of the LPS-induced IL-1 beta expression by NF-kappaB inhibitor pyrrolidine dithiocarbamate (PDTC). Cortisol was able to inhibit in vitro constitutive expression of IL-1 beta transcripts. Addition of cortisol simultaneously with LPS could not substantially inhibit transcription. (C) 2001 Elsevier Science Ltd. All rights reserved.",
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    AU - Engelsma, M.

    AU - Stet, Rene Josephus Maria

    AU - Schipper, H.

    AU - Verburg-van Kemenade, B. M. L.

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    N2 - The intron-exon organisation of the carp IL-1 beta gene consists of 2455 bp and comprises seven exons. Three IL-1 beta RNA transcripts have been found in carp: (1) a fully spliced product; (2) exon 1-7 with introns 5 and 6; and (3) exon 1-7 with intron 5 only. The intron-containing products probably represent partially spliced transcripts. IL-1 beta mRNA expression in carp was semi-quantitatively analysed by RT-PCR in multiple organs, including brain and pituitary. Constitutive expression of the IL-1 beta mRNA was found in these organs with a predominant expression in the immune organs head kidney and spleen. Furthermore, a scattered distribution of IL-1 beta producing cells was shown by in situ hybridisations of head kidney tissue. Administration of phorbol-myristate-acetate (PMA), lipopolysaccharide (LPS) or retinoic acid (RA), to phagocytes isolated from the head kidney, resulted in expression of IL-1 beta intron-containing transcripts. Of these, only PMA and LPS were stimulators that induced the fully spliced transcript. A role for the nuclear factor (NF)-kappaB pathway in carp IL-1 beta expression was shown with suppression of the LPS-induced IL-1 beta expression by NF-kappaB inhibitor pyrrolidine dithiocarbamate (PDTC). Cortisol was able to inhibit in vitro constitutive expression of IL-1 beta transcripts. Addition of cortisol simultaneously with LPS could not substantially inhibit transcription. (C) 2001 Elsevier Science Ltd. All rights reserved.

    AB - The intron-exon organisation of the carp IL-1 beta gene consists of 2455 bp and comprises seven exons. Three IL-1 beta RNA transcripts have been found in carp: (1) a fully spliced product; (2) exon 1-7 with introns 5 and 6; and (3) exon 1-7 with intron 5 only. The intron-containing products probably represent partially spliced transcripts. IL-1 beta mRNA expression in carp was semi-quantitatively analysed by RT-PCR in multiple organs, including brain and pituitary. Constitutive expression of the IL-1 beta mRNA was found in these organs with a predominant expression in the immune organs head kidney and spleen. Furthermore, a scattered distribution of IL-1 beta producing cells was shown by in situ hybridisations of head kidney tissue. Administration of phorbol-myristate-acetate (PMA), lipopolysaccharide (LPS) or retinoic acid (RA), to phagocytes isolated from the head kidney, resulted in expression of IL-1 beta intron-containing transcripts. Of these, only PMA and LPS were stimulators that induced the fully spliced transcript. A role for the nuclear factor (NF)-kappaB pathway in carp IL-1 beta expression was shown with suppression of the LPS-induced IL-1 beta expression by NF-kappaB inhibitor pyrrolidine dithiocarbamate (PDTC). Cortisol was able to inhibit in vitro constitutive expression of IL-1 beta transcripts. Addition of cortisol simultaneously with LPS could not substantially inhibit transcription. (C) 2001 Elsevier Science Ltd. All rights reserved.

    KW - fish

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    KW - gene expression

    KW - LPS

    KW - cortisol

    KW - NEUTROPHILIC GRANULOCYTES

    KW - GENE-EXPRESSION

    KW - MESSENGER-RNA

    KW - MOLECULAR-CLONING

    KW - RETINOIC ACID

    KW - MACROPHAGES

    KW - SEQUENCE

    KW - FISH

    KW - APOPTOSIS

    KW - SYSTEM

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    DO - 10.1016/S0145-305X(00)00059-8

    M3 - Article

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    SP - 195

    EP - 203

    JO - Developmental and Comparative Immunology

    JF - Developmental and Comparative Immunology

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    ER -