Regulation of interleukin 1 beta expression in the common carp, Cyprinus carpio L.

M. Engelsma, Rene Josephus Maria Stet, H. Schipper, B. M. L. Verburg-van Kemenade

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    Abstract

    The intron-exon organisation of the carp IL-1 beta gene consists of 2455 bp and comprises seven exons. Three IL-1 beta RNA transcripts have been found in carp: (1) a fully spliced product; (2) exon 1-7 with introns 5 and 6; and (3) exon 1-7 with intron 5 only. The intron-containing products probably represent partially spliced transcripts. IL-1 beta mRNA expression in carp was semi-quantitatively analysed by RT-PCR in multiple organs, including brain and pituitary. Constitutive expression of the IL-1 beta mRNA was found in these organs with a predominant expression in the immune organs head kidney and spleen. Furthermore, a scattered distribution of IL-1 beta producing cells was shown by in situ hybridisations of head kidney tissue. Administration of phorbol-myristate-acetate (PMA), lipopolysaccharide (LPS) or retinoic acid (RA), to phagocytes isolated from the head kidney, resulted in expression of IL-1 beta intron-containing transcripts. Of these, only PMA and LPS were stimulators that induced the fully spliced transcript. A role for the nuclear factor (NF)-kappaB pathway in carp IL-1 beta expression was shown with suppression of the LPS-induced IL-1 beta expression by NF-kappaB inhibitor pyrrolidine dithiocarbamate (PDTC). Cortisol was able to inhibit in vitro constitutive expression of IL-1 beta transcripts. Addition of cortisol simultaneously with LPS could not substantially inhibit transcription. (C) 2001 Elsevier Science Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)195-203
    Number of pages8
    JournalDevelopmental and Comparative Immunology
    Volume25
    DOIs
    Publication statusPublished - 2001

    Keywords

    • fish
    • Cyprinus carpio
    • interleukin 1
    • mRNA
    • gene expression
    • LPS
    • cortisol
    • NEUTROPHILIC GRANULOCYTES
    • GENE-EXPRESSION
    • MESSENGER-RNA
    • MOLECULAR-CLONING
    • RETINOIC ACID
    • MACROPHAGES
    • SEQUENCE
    • FISH
    • APOPTOSIS
    • SYSTEM

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