Abstract
The intron-exon organisation of the carp IL-1 beta gene consists of 2455 bp and comprises seven exons. Three IL-1 beta RNA transcripts have been found in carp: (1) a fully spliced product; (2) exon 1-7 with introns 5 and 6; and (3) exon 1-7 with intron 5 only. The intron-containing products probably represent partially spliced transcripts. IL-1 beta mRNA expression in carp was semi-quantitatively analysed by RT-PCR in multiple organs, including brain and pituitary. Constitutive expression of the IL-1 beta mRNA was found in these organs with a predominant expression in the immune organs head kidney and spleen. Furthermore, a scattered distribution of IL-1 beta producing cells was shown by in situ hybridisations of head kidney tissue. Administration of phorbol-myristate-acetate (PMA), lipopolysaccharide (LPS) or retinoic acid (RA), to phagocytes isolated from the head kidney, resulted in expression of IL-1 beta intron-containing transcripts. Of these, only PMA and LPS were stimulators that induced the fully spliced transcript. A role for the nuclear factor (NF)-kappaB pathway in carp IL-1 beta expression was shown with suppression of the LPS-induced IL-1 beta expression by NF-kappaB inhibitor pyrrolidine dithiocarbamate (PDTC). Cortisol was able to inhibit in vitro constitutive expression of IL-1 beta transcripts. Addition of cortisol simultaneously with LPS could not substantially inhibit transcription. (C) 2001 Elsevier Science Ltd. All rights reserved.
Original language | English |
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Pages (from-to) | 195-203 |
Number of pages | 8 |
Journal | Developmental and Comparative Immunology |
Volume | 25 |
DOIs | |
Publication status | Published - 2001 |
Keywords
- fish
- Cyprinus carpio
- interleukin 1
- mRNA
- gene expression
- LPS
- cortisol
- NEUTROPHILIC GRANULOCYTES
- GENE-EXPRESSION
- MESSENGER-RNA
- MOLECULAR-CLONING
- RETINOIC ACID
- MACROPHAGES
- SEQUENCE
- FISH
- APOPTOSIS
- SYSTEM