Regulation of metallothionein gene expression and secretion in rat adipocytes differentiated from preadipocytes in primary culture

P Trayhurn, Jackie Duncan, A M Wood, J H Beattie

Research output: Contribution to journalLiterature review

39 Citations (Scopus)

Abstract

The gene encoding metallothionein, a low mel. wt. metal binding and stress response protein, is expressed in white adipose tissue. In the present study, metallothionein (MT-1) gene expression and factors regulating metallothionein production have been examined in adipocytes induced to differentiate from fibroblastic preadipocytes in primary cell culture. On the induction of differentiation, the metallothionein-1 gene was strongly expressed in the cells and metallothionein released into the medium. A peak in metallothionein-1 mRNA level and metallothionein secretion occurred at 2 and 10 days post-differentiation, respectively, with a decrease in protein release after this time. The metallothionein-1 gene was expressed in the adipocytes prior to the adipsin and lipoprotein lipase genes, suggesting that it is an early marker of adipocyte differentiation. The addition of the glucocorticoid. dexamethasone, led to a substantial increase in metallothionein-1 mRNA in the cells and metallothionein secretion. Insulin and leptin also stimulated metallothionein production, although the effect was small. Neither noradrenaline nor the beta3-adrenoceptor agonist, BRL 37 344, altered metallothionein release but forskolin and bromo-cAMP were stimulatory, markedly increasing both metallothionein-1 level and metallothionein secretion. It is suggested that metallothionein is a novel secretory product of the differentiated white adipocyte and that its production is regulated particularly by glucocorticoids and through a cAMP-dependent pathway.

Original languageEnglish
Pages (from-to)542-547
Number of pages6
JournalHormone and Metabolic Research
Volume32
Issue number11-12
Publication statusPublished - 2000

Keywords

  • antioxidant
  • cAMP
  • catecholamines
  • glucocorticoids
  • leptin
  • white adipose tissue
  • WHITE ADIPOSE-TISSUE
  • OB GENE
  • LIPOPROTEIN-LIPASE
  • COLD-EXPOSURE
  • FEMALE MICE
  • OBESE GENE
  • BROWN FAT
  • LEPTIN
  • ENDOCRINE
  • CELL

Cite this

Regulation of metallothionein gene expression and secretion in rat adipocytes differentiated from preadipocytes in primary culture. / Trayhurn, P ; Duncan, Jackie; Wood, A M ; Beattie, J H .

In: Hormone and Metabolic Research, Vol. 32, No. 11-12, 2000, p. 542-547.

Research output: Contribution to journalLiterature review

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AU - Trayhurn, P

AU - Duncan, Jackie

AU - Wood, A M

AU - Beattie, J H

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N2 - The gene encoding metallothionein, a low mel. wt. metal binding and stress response protein, is expressed in white adipose tissue. In the present study, metallothionein (MT-1) gene expression and factors regulating metallothionein production have been examined in adipocytes induced to differentiate from fibroblastic preadipocytes in primary cell culture. On the induction of differentiation, the metallothionein-1 gene was strongly expressed in the cells and metallothionein released into the medium. A peak in metallothionein-1 mRNA level and metallothionein secretion occurred at 2 and 10 days post-differentiation, respectively, with a decrease in protein release after this time. The metallothionein-1 gene was expressed in the adipocytes prior to the adipsin and lipoprotein lipase genes, suggesting that it is an early marker of adipocyte differentiation. The addition of the glucocorticoid. dexamethasone, led to a substantial increase in metallothionein-1 mRNA in the cells and metallothionein secretion. Insulin and leptin also stimulated metallothionein production, although the effect was small. Neither noradrenaline nor the beta3-adrenoceptor agonist, BRL 37 344, altered metallothionein release but forskolin and bromo-cAMP were stimulatory, markedly increasing both metallothionein-1 level and metallothionein secretion. It is suggested that metallothionein is a novel secretory product of the differentiated white adipocyte and that its production is regulated particularly by glucocorticoids and through a cAMP-dependent pathway.

AB - The gene encoding metallothionein, a low mel. wt. metal binding and stress response protein, is expressed in white adipose tissue. In the present study, metallothionein (MT-1) gene expression and factors regulating metallothionein production have been examined in adipocytes induced to differentiate from fibroblastic preadipocytes in primary cell culture. On the induction of differentiation, the metallothionein-1 gene was strongly expressed in the cells and metallothionein released into the medium. A peak in metallothionein-1 mRNA level and metallothionein secretion occurred at 2 and 10 days post-differentiation, respectively, with a decrease in protein release after this time. The metallothionein-1 gene was expressed in the adipocytes prior to the adipsin and lipoprotein lipase genes, suggesting that it is an early marker of adipocyte differentiation. The addition of the glucocorticoid. dexamethasone, led to a substantial increase in metallothionein-1 mRNA in the cells and metallothionein secretion. Insulin and leptin also stimulated metallothionein production, although the effect was small. Neither noradrenaline nor the beta3-adrenoceptor agonist, BRL 37 344, altered metallothionein release but forskolin and bromo-cAMP were stimulatory, markedly increasing both metallothionein-1 level and metallothionein secretion. It is suggested that metallothionein is a novel secretory product of the differentiated white adipocyte and that its production is regulated particularly by glucocorticoids and through a cAMP-dependent pathway.

KW - antioxidant

KW - cAMP

KW - catecholamines

KW - glucocorticoids

KW - leptin

KW - white adipose tissue

KW - WHITE ADIPOSE-TISSUE

KW - OB GENE

KW - LIPOPROTEIN-LIPASE

KW - COLD-EXPOSURE

KW - FEMALE MICE

KW - OBESE GENE

KW - BROWN FAT

KW - LEPTIN

KW - ENDOCRINE

KW - CELL

M3 - Literature review

VL - 32

SP - 542

EP - 547

JO - Hormone and Metabolic Research

JF - Hormone and Metabolic Research

SN - 0018-5043

IS - 11-12

ER -