Rep provides a second motor at the replisome to promote duplication of protein-bound DNA

Colin P Guy, John Atkinson, Milind K Gupta, Akeel A Mahdi, Emma J Gwynn, Christian J Rudolph, Peter B Moon, Ingeborg C van Knippenberg, Chris J Cadman, Mark S Dillingham, Robert G Lloyd, Peter McGlynn

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Nucleoprotein complexes present challenges to genome stability by acting as potent blocks to replication. One attractive model of how such conflicts are resolved is direct targeting of blocked forks by helicases with the ability to displace the blocking protein-DNA complex. We show that Rep and UvrD each promote movement of E. coli replisomes blocked by nucleoprotein complexes in vitro, that such an activity is required to clear protein blocks (primarily transcription complexes) in vivo, and that a polarity of translocation opposite that of the replicative helicase is critical for this activity. However, these two helicases are not equivalent. Rep but not UvrD interacts physically and functionally with the replicative helicase. In contrast, UvrD likely provides a general means of protein-DNA complex turnover during replication, repair, and recombination. Rep and UvrD therefore provide two contrasting solutions as to how organisms may promote replication of protein-bound DNA.
Original languageEnglish
Pages (from-to)654-666
Number of pages13
JournalMolecular Cell
Volume36
Issue number4
DOIs
Publication statusPublished - 25 Nov 2009

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Nucleoproteins
DNA
Proteins
Recombinational DNA Repair
Genomic Instability
Escherichia coli
In Vitro Techniques

Keywords

  • culture media
  • DNA helicases
  • DNA replication
  • DNA, bacterial
  • DNA-directed DNA polymerase
  • DnaB helicases
  • Escherichia coli
  • Escherichia coli proteins
  • genetic complementation test
  • molecular motor proteins
  • multienzyme complexes
  • mutation
  • nucleoproteins
  • protein binding
  • suppression, genetic
  • transcription, genetic

Cite this

Guy, C. P., Atkinson, J., Gupta, M. K., Mahdi, A. A., Gwynn, E. J., Rudolph, C. J., ... McGlynn, P. (2009). Rep provides a second motor at the replisome to promote duplication of protein-bound DNA. Molecular Cell, 36(4), 654-666. https://doi.org/10.1016/j.molcel.2009.11.009

Rep provides a second motor at the replisome to promote duplication of protein-bound DNA. / Guy, Colin P; Atkinson, John; Gupta, Milind K; Mahdi, Akeel A; Gwynn, Emma J; Rudolph, Christian J; Moon, Peter B; van Knippenberg, Ingeborg C; Cadman, Chris J; Dillingham, Mark S; Lloyd, Robert G; McGlynn, Peter.

In: Molecular Cell, Vol. 36, No. 4, 25.11.2009, p. 654-666.

Research output: Contribution to journalArticle

Guy, CP, Atkinson, J, Gupta, MK, Mahdi, AA, Gwynn, EJ, Rudolph, CJ, Moon, PB, van Knippenberg, IC, Cadman, CJ, Dillingham, MS, Lloyd, RG & McGlynn, P 2009, 'Rep provides a second motor at the replisome to promote duplication of protein-bound DNA', Molecular Cell, vol. 36, no. 4, pp. 654-666. https://doi.org/10.1016/j.molcel.2009.11.009
Guy CP, Atkinson J, Gupta MK, Mahdi AA, Gwynn EJ, Rudolph CJ et al. Rep provides a second motor at the replisome to promote duplication of protein-bound DNA. Molecular Cell. 2009 Nov 25;36(4):654-666. https://doi.org/10.1016/j.molcel.2009.11.009
Guy, Colin P ; Atkinson, John ; Gupta, Milind K ; Mahdi, Akeel A ; Gwynn, Emma J ; Rudolph, Christian J ; Moon, Peter B ; van Knippenberg, Ingeborg C ; Cadman, Chris J ; Dillingham, Mark S ; Lloyd, Robert G ; McGlynn, Peter. / Rep provides a second motor at the replisome to promote duplication of protein-bound DNA. In: Molecular Cell. 2009 ; Vol. 36, No. 4. pp. 654-666.
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